International Research Journal of Engineering and Technology (IRJET)
e-ISSN: 2395 -0056
Volume: 04 Issue: 03| Mar -2017
p-ISSN: 2395-0072
www.irjet.net
IN-SILICO ANALYSIS AND DOCKING OF HUMAN SERUM PROTEIN WITH QUERCETIN IN T2DM Uma Maheswari.M1, B. Sai Shankar2, Dr. C. Joyce Priyakumari3 1Research
Assistant, Bioinformatics Infrastructure Facility (BIF), BTIS Net, Madras Christian College, Chennai. Bioinformatics Infrastructure Facility (BIF), BTIS Net, Madras Christian College, Chennai. 3*Corresponding Author, Assistant Professor, Department of Zoology, Co-coordinator, Bioinformatics Infrastructure Facility (BIF), BTIS Net, Madras Christian College, Chennai, Tamil Nadu, India. 2Trainee,
---------------------------------------------------------------------***--------------------------------------------------------------------capers, lovage, dock and raddish leaves and in red onions, higher concentrations of quercetin occur in the human serum albumin in turn switches on the gene in the islet of Langerhans in the pancreas which induces the beta cells to outermost rings and in the part closest to the root, the produce insulin. This helps to lower the sugar levels in T2DM latter being the part of the plant with the highest (type 2 Diabetes mellitus) patients. The interaction between concentration [4]. Quercetin, the most commonly found Quercetin and human serum protein has been studied in detail flavonoid in the human diet, has been shown to exert using the Autodock (vina) software. Quercetin binds to the potent free radical scavenging and antioxidant actions, human serum protein and the docking energy for the process to cause vasodilatation in isolated vascular was recorded to be -9.2 kcal/mol. preparations, and to elicit blood glucose-lowering (anti-diabetic) effects in experimental diabetes [5]-[9]. Key Words: Quercetin, T2DM, Autodock (vina), Docking Based on several in vitro, animal models and some human studies, dietary plant polyphenols and polyphenol-rich products modulate carbohydrate and 1.INTRODUCTION lipid metabolism, attenuate hyperglycemia, Diabetes has been one of the most prevalent conditions dyslipidemia and insulin resistance, improve adipose in Asian pacific region. There are two types of diabetes tissue metabolism, and alleviate oxidative stress and Type 1 and Type 2. Among these, type 2 has been stress-sensitive signaling pathways and inflammatory considered as chronic progressive state. During this processes. Polyphenolic compounds can also prevent state the plasma glucose rises gradually in spite of the the development of long-term diabetes complications type of treatment [1]. including cardiovascular disease, neuropathy, Type 2 diabetes is one of the major public health nephropathy and retinopathy. Further investigations as concerns in both developing and developed countries human clinical studies are needed to obtain the in the Asian-Pacific region. It has become epidemic in a optimum dose and duration of supplementation with number of countries, particularly in newly polyphenolic compounds in diabetic patients [10]. industrialized nations. Healthcare budgets of many Significant evidence suggests that countries have been affected as a result of the direct polyphenol-rich diets have the ability to protect against and indirect social and economic costs of treating diabetes. The benefits of dietary polyphenols for type 2 diabetes and its complications. Type 2 diabetes results diabetes can be summarized as: protection of in the following symptoms premature morbidity and pancreatic β-cells against glucose toxicity, antimortality, particularly from cardiovascular disease inflammatory and antioxidant effects, inhibition of α(CVD), blindness, amputations and renal failure. Also it amylases or α- glucosidases and thus decrease of starch is a part of the metabolic syndrome, a cluster of major digestion, and inhibition of advanced glycation end CVD risk factors previously also referred to as the products formation [11]. Polyphenols, especially the insulin resistance syndrome, Syndrome X or the Deadly large polymeric type or condensed tannins, appear to Quartet [2]. be responsible in part for the reduced glycemic Quercetin (Pubchem CID: 5280343) is a polyphenolic response to carbohydrate foods [12]. In this paper we flavonoid with potential chemopreventive activity. have made an attempt to study detailed interaction Quercetin, ubiquitous in plant food sources and a major between human serum albumin and Quercetin. bioflavonoid in the human diet [3]. Quercetin is rich in
Abstract - Quercetin acts on human serum protein. The
© 2017, IRJET
|
Impact Factor value: 5.181
|
ISO 9001:2008 Certified Journal
|
Page 2816