International Research Journal of Engineering and Technology (IRJET)
e-ISSN: 2395 -0056
Volume: 04 Issue: 01 | Jan -2017
p-ISSN: 2395-0072
www.irjet.net
Synthesis of the silver nanoparticles from Aloe barbadensis extract and its application against the urinogenial tract infection Karthikeyen T R S, 1B.tech
(biotechnology), K.S.Rangasamy college of technology Thiruchengode ------------------------------------------------------------------------------------------------------------------------------------------Abstract-The integration of nanoparticles with the field of INTRODUCTION biotechnology is finding the wide range of applicability in various area of medical sciences. Recently the silver nanoparticles have gained the attention because of their antimicrobial activity which offers the possibility of their use for medicinal purposes. This study aims at evaluating the antimicrobial properties of green synthesized silver nanoparticles (AgNP’s) from Aloe barbadensis leaf extract is treated against urinary tract infection pathogens [1]. About thirty-two bacteria are isolated from the mid urine sample of 25 male and 25 female patients from erode district, Tamilnadu, India and are identified by the conventional methods. The prepared nanoparticles were characterized by FT-IR and UV– Vis spectroscopy [2]. FT-IR study showed sharp absorption peaks at 1,631 and 3,433 cm-1 for amide and alcoholic hydroxide groups, respectively. Escherichia coli was found predominant in account of 47% sensitivity. The antibacterial activity of silver nanoparticles are evaluated by the disc diffusion assay and well diffusion method. Escherichia coli has maximum sensitivity (11±0.58mm) followed by candida albican (8±0.49mm) at a concentration of 20µg disc-1 and the sensitivity was highly comparable with the positive control chloramphenicol and streptomycin. K.pneumoniae and S.aureus showed no sensitivity to any concentrations of the silver nanoparticles. The result provided by the experiment shows that the silver nanoparticles are potentially used for the treatment of the urinary tract infections (UTIs) which are caused by the streptococcus aureus, Escherichia coli Candia albicans, Klebsiella pneumonia. However, microscopic observation revealed that synthesized nanoparticles caused detrimental effects on conidial germination along with other deformations such as structure of cell membrane and inhibited normal budding process of the tested species. Therefore, it has been concluded that Aloe barbedensis leaf extract origin silver nanoparticles have tremendous potentiality towards controlling urinary tract infection causing pathogens [3]. However, further research is needed to check the efficacy of size dependent AgNPs on different species of bacteria and fungus. Key Words: silver nanoparticle-Green synthesis –Aloe Vera leaf -Antimicrobial effect –disc diffusion streptococcus aureus-Escherichia coli -Candia albicansKlebsiella pneumonia © 2017, IRJET
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Impact Factor value: 5.181
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The field of nanotechnology is one of the most industrious areas of research in current material sciences. Nanotechnology is a field is improving day by day, making an impact in all spheres of human life especially medical science and biotechnology. Nanoparticles exhibit completely new or improved properties based on specific characteristics such as size, distribution and morphology. Nanoparticles of noble metals, such as gold, silver, platinum, and zinc oxide are widely applied in products that directly come in contact with the human body, such as detergent, cosmetic products, and toothpaste, besides medical and pharmaceutical applications. Nanoparticle formation has been reported using chemical and physical methods. There are various methods for NPs formation such as sol-gel process, chemical precipitation, hydrothermal method, microwave, chemical vapour deposition, the above methods involve the usage of hazardous reagents for synthesis of nanoparticles. In view of an environmental sustenance, there is an urgent need to develop an eco-friendly method of synthesis of nanomaterial Therefore, there is a growing need to develop environmentally friendly processes for nanoparticle synthesis without using toxic chemicals. Biological methods for nanoparticle synthesis using microorganisms, enzymes, and plants or plant extracts have been suggested as possible eco-friendly alternatives to chemical and physical methods. Biological methods of synthesis have paved way for the “greener synthesis” of nanoparticles and these have proven to be better methods due to slower kinetics, they offer better manipulation and control over crystal growth and their stabilization. This has motivated an upsurge in research on the synthesis routes that allow better control of shape and size for various Nano technological applications. The use of materials like plant extract bacteria, fungi and enzymes for the synthesis of silver nanoparticles offer numerous benefits of eco-friendliness and compatibility for pharmaceutical and other biomedical applications as they do not use toxic chemicals for the synthesis protocol. Silver has long been used as a disinfectant; for example, the metal has been used in treating wounds and burns because of its broad-spectrum toxicity to bacteria [4]. Silver nanoparticles have unique catalytic, optical, electrical and antimicrobial properties, which inhibiting 650 types of microbe’s growth as well as because of its reputation of limited toxicity to humans . Various methods are available for synthesis of silver nanoparticles, which sign thereto above. various plant extracts such as Cinnamon camphora, Cinnamon zeylanicum, ISO 9001:2008 Certified Journal
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