International Research Journal of Engineering and Technology (IRJET)
e-ISSN: 2395-0056
Volume: 04 Special Issue: 09 | Sep -2017
p-ISSN: 2395-0072
www.irjet.net
One Day International Seminar on Materials Science & Technology (ISMST 2017) 4th August 2017 Organized by
Department of Physics, Mother Teresa Women’s University, Kodaikanal, Tamilnadu, India
Green Synthesis Of Silver Nanoparticles And Its Antibacterial Activity M. R. Bindhu1*, Beena.M.Amala1 and M.Jeeva2 1Department
of Physics, Nanjil Catholic College of Arts and Science, Nedumcode, Kaliyakavilai, Tamil Nadu, India. center of Physics, Jayaraj Annapakiam College for Women (Autonomous), Periyakulam. ---------------------------------------------------------------------***--------------------------------------------------------------------2Research
Abstract - An ecofriendly method of obtaining spherical
silver nanoparticles (AgNPs) has been synthesized, using Carica papaya fruit extract as reducing agent. The biomolecules present in the extract was responsible for reduction of Ag+ ions from AgNO3. The prepared nanoparticles were characterized by UV–visible spectroscopy (UV–vis), XRay Diffraction pattern (XRD), Transmission Electron Microscopy (TEM) and Energy dispersive spectroscopy (EDS) technique to identify the size and shape of nanoparticles. The prepared AgNPs were monodispersed, spherical in shape with the average particle size of 16 nm and shows surface plasmon resonance (SPR) peak around 429nm. The consequences of the antibacterial studies confirm the superior ability of prepared silver nanoparticles to inhibit the growth of bacteria. Key Words: Carica papaya, Optical properties, Surface Plasmon Resonance, antibacterial activity.
1.INTRODUCTION In recent years, microorganisms have developed to become drug resistant by the alterations in their chromosomes/genetic materials. Emergence of the antibiotic resistance pathogens has become a severe healthiness problem and thus, many studies have been reported to develop the present antimicrobial treatments. It is known that over 70% of bacterial infections are resistant to one or more of the antibiotics that are usually used to eliminate the infection. These troubles require the growth of new effectual antibacterial agents against new generation bacteria. A wellorganized method to estimate nanotoxicity is to examine the reaction of bacteria exposed to nanoparticles. So it will be helpful, if an easy mechanism can be intended to inhibit the development of various pathogenic bacteria. Antimicrobial agents are of immense significance in numerous industries such as water disinfection, packaging, textiles, construction, medicine and food. The influence of green synthesized metal nanoparticles on bacteria is very important since they constitute the lowest level and hence enter the food chain of the ecosystems. Among the known nanoparticles, silver has been widely studied because of their unique optical, electrical, and photothermal properties. In recent years, green synthesis approach of AgNPs, using plants have © 2017, IRJET
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received great attention to chemical and physical methods. Recently, extracts of Solanum lycopersicums, Hibiscus cannabinus stem, Hibiscus cannabinus leaf, Ananas comosus, and Hibiscus cannabinus leaf have found appropriate for the green synthesis of nanoparticles [1-5]. Green synthesized AgNPs has been reported to show antibacterial activity against different types of bacteria [3-9]. Carica papaya belongs to family Caricaceae and commonly known as Papaya. It is one of the medicinal plants. The papaya fruits, bark, leaves are being used as medicine to treat various diseases such as warts, corns, constipation, amenorrhoea, general debility, sinuses, eczema, cutaneous tubercles, glandular tumours, blood pressure, dyspepsia, cancer cell growth, diabetes, malaria expel worms and stimulate reproductive organs, syphilis and gonorrhea. Carica papaya fruit extracts are being used to treat dengue fever. Carica papaya extract exerting antioxidant and immune stimulant properties against acrylamide toxicity in rats [10-11]. Major aim of this present study is to synthesize AgNPs using green synthesis method and show its function as an antibacterial agent so that it may be used for biomedical application.
2.EXPERIMENTAL DETAILS An aqueous solution of AgNO3 (1mM) was added to 2ml of Papaya fruit extract and stirred for 40 min at room temperature. It initially becomes colorless and turned into reddish brown indicating the formation of AgNPs (S1). The dried samples were characterized by XRD using X’Per PRO (PANalytical) X-ray Diffractometer with CuKα radiation λ =1.5406A˚, at the scanning rate of 0.050. UV study was carried out by using Double Beam UV-vis Spectrophotometer (LMSP UV 1900). Morphology studies were made using a JEOL TEM 2010 High Resolution TEM with an accelerating voltage of 200 kV. A disc diffusion method was used to evaluate the antibacterial action of prepared NPs against bacteria namely Staphylococcus aureus (gram positive).
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