International Research Journal of Engineering and Technology (IRJET)
e-ISSN: 2395-0056
Volume: 04 Special Issue: 09 | Sep -2017
p-ISSN: 2395-0072
www.irjet.net
One Day International Seminar on Materials Science & Technology (ISMST 2017) 4th August 2017 Organized by
Department of Physics, Mother Teresa Women’s University, Kodaikanal, Tamilnadu, India
ACOUSTICS THERMODYNAMIC AND SOLVATION STUDY OF PROTEIN SALT SOLUTIONS USING ULTRASONIC TECHNIQUE Dr.Kannagi.K1, Ilavarasi2 1,2PG
Department of Physics - Cauvery College for Women, Trichy, Tamil Nadu, India ---------------------------------------------------------------------***---------------------------------------------------------------------
Abstract - Ultrasonic investigation finds extensive
N-p-Tosyl-L-Arginine methyl ester hydrochloride (LTAME) increases nitric oxide production and acts as a vasodilator.5 It can be utilized as a substrate for the serine proteases trypsin, plasmin and thrombin. It has been used as a protease inhibitor,6 L-TAME has also been described to be a possible new cardiovascular risk factor among smokers.7
applications in probing in to the physico-chemical behavior of liquids leading to an understanding of the liquid state. This technique has been adequately employed to study the properties of any substance to understand the nature of molecular interactions in pure liquids and ionic interactions in protein salt solutions. The measurements of ultrasonic velocity of liquid play an important role in understanding the nature of molecular systems. The present work deals with the molecular interaction studies on some amino acids in non-aqueous solutions at various temperatures. From the measured parameters such as velocity, density, viscosity and some derived parameters like internal pressure, free volume, Rao’s constant and Wada’s constant are calculated. The study reveals the structural changes occurring in the solution through the FTIR study of L-Arginine derivatives in polar solvent. The diagnostic bonds observed at certain frequencies are assigned to the carbonyl stretch present is identified. The assignment of FTIR spectra has thrown light on the interaction between the solute and the solvent
2. Experimental Technique: L.Arginine solutions and polar-protic solvent are used in the present study. With the high purity(99%) the samples are purchased from siscom research laboratories,Mumbai. The samples are measured using an electronic balance precise to 0.0001gm. Ultrasonic velocity (u) of L.Arginine salts in formamide is measured with a variable path interferometer (2MHZ) with an accuracy of ±2m/s. The density (ρ) measurement is made with an accuracy of ±0.001gm.The(i) 2 7 k 1 3 6 atms. / M eff u 2
Internal pressure i bRT
Keyword: Adiabatic compressibility, Solvation effect, FTIR spectral analysis
F (ii) Free volume
1. Introduction
(iii) Rao’s constant
Amino acids (NH2-CH-COOH-R, where R is a radical) are the basic units of proteins and peptides.1For an unknown reason nature has chosen 20 of these special molecules, differing in the R part, to form the impressive number of proteins found in our planet. Among other amino acids L-Arginine and its salts are known to protect protein. Due to the biological importance and large scale applications, L-Arginine and its derivatives are considered as samples for the study. Hence the study of these compounds in non-aqueous solutions is a new attempt, to understand the significant effects of salts on bio-molecules. Nα-Benzoyl - L-Arginine (L-NBA) is an essential component of important biological molecules.2It is used in the study of peptide science and protein engineering which places significant demands. Nα-Benzoyl L-Arginine was originally developed by Erlanger3 et al. as a trypsin substrate, is now widely used in the estimation of α 1– antitrypsin. L-NBA is also necessary for inhibitory action.4
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(iv) Wada’s constant
(v) Adiabatic Compressibility
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M eff (U )
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W
(vi) Solvation Number
V f M eff u / k 2 cc
M eff ( )
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1 2 cm 2 / dyne u
n n h s 1 n i 0
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