International Research Journal of Engineering and Technology (IRJET)
e-ISSN: 2395-0056
Volume: 04 Issue: 08 | Aug -2017
p-ISSN: 2395-0072
www.irjet.net
FACILE SYNTHESIS OF CuO NANOPARTICLES BY HYDROTHERMAL METHOD AND THEIR APPLICATION ON ANTIBACTERIAL ACTIVITY V. Maria Vinosel, A. Persis Amaliya, S. Blessi, S. Pauline* Department of Physics, Loyola College, University of Madras, Chennai – 34, India *Corresponding author: paulantovero@yahoo.co.in ---------------------------------------------------------------------***---------------------------------------------------------------------
Abstract - A versatile and surfactant free hydrothermal
method was adopted to synthesize CuO nanoparticles. Using hydrothermal method CuO nanoparticles can be synthesized without organic solvents, expensive raw materials and complicated equipments. CuO nanoparticles were characterized by X-ray diffraction analysis (XRD), High resolution scanning electron microscopy (HRSEM), Energy dispersive X-ray analysis (EDAX), Fourier transform infrared (FTIR) and UV- visible spectroscopy. Structural analysis reveals that CuO nanoparticles have monoclinic structure with single crystalline phase. High resolution scanning electron microscopy exhibits rod like CuO nanostructures. Fourier Transform Infrared Spectroscopy spectra showed the vibrational bands of Cu-O metal oxygen bonds. Absorption spectra of CuO nanoparticles show broad absorption bands and band gap energy were determined. Antibacterial behaviors of CuO nanoparticles were also examined. Key Words: Copper Oxide, Nanorod, Monoclinic, Energy gap, Antibacterial
1. INTRODUCTION CuO is a p-type semiconductor with a narrow band gap of 1.2eV is one of the most intensively studied metal oxide because of its potential applications in catalysis, solar cells, magnetic storage media, semiconductors, field transistors, gas sensors, batteries etc [1-3]. The surface morphology, size, shape and crystal structure of nanomaterials are important parameters that influence their chemical, optical and electrical properties [4]. Many methods have been developed for the preparation of CuO nanostructures including hydrothermal [5], thermal oxidation [6], electrochemical [7], solvothermal [8], simple hydrolysis [9], Microwave assisted and sol-gel [10] methods. Xiao et al. [11] synthesized CuO nanorods by using hydrothermal route in presence of sodium citrate. Liu and Bando et al. [12] synthesized nanodendrite and nanoshuttle like CuO nanostructures under surfactant assisted condition by hydrothermal route. However, hydrothermal solution phase synthesis, which has advantages such as low temperature, versatile synthetic process, great potential for scaling up, low-energy requirements, safe and environmentally benign synthetic conditions [13]. In this work, hydrothermal method was employed to synthesize CuO nanorod. The structure, morphology, elemental composition and optical properties of the as© 2017, IRJET
|
Impact Factor value: 5.181
|
prepared sample were characterized by XRD, HRSEM, EDX and UV-Vis. The antibacterial activity was also investigated.
2. EXPERIMENTAL All the reagents used in the experiment were of analytical grade purity. CuO nanoparticles were prepared by hydrothermal method with Sodium hydroxide (NaOH) as precipitating agent. Copper precursor solution was prepared in a beaker by addition of 0.2M Cucl2.2H2O and 0.4M of sodium hydroxide dissolved in 40ml of double distilled water. The aqueous solution of NaOH was added dropwise into the above solution under constant stirring. The pH value of the solution was maintained to be 10. The resultant solution was transferred to the stainless steel autoclave and hydrothermal synthesis was carried out for about 150°C for 12h. The obtained black precipitate was washed several times with deionized water and acetone to remove impurities. The final product is dried at 75°C for 6h.
3. ANTIBACTERIAL PERFORMANCE 30 mg of the CuO nanoparticles was suspended in 3.0 ml of Milli Q water to get a stock suspension of 10 mg/mL. From this stock suspension six different working suspensions of the concentrations 100, 200,300, 400, 500 and 600 µg were prepared with sterile deionized water in sterile test tubes. Using sterile cotton swab, the subculture of Staphylococcus aureus was made as a suspension in sterile Tryptone Soya broth. Using sterile micro tip7shallow wells were punched in the culture media with a gap of 1 cm between each well. The wells were marked at the base of the plate as 1,2,3,4,5,6 and control. With sterile micro tip 100 µL of each one of the 6 working CuO nanoparticle suspension was added to the duly marked wells. The bacterium was also exposed to an antibiotic disc containing 5µg of Ciprofloxacin, as an antibiotic control. The culture plate was incubated in the bacteriological incubator [Equitron] at 37°C for 24 h. After the incubation period the plate was examined for zone of inhibition.
4. RESULTS AND DISCUSSION 4.1 X-ray diffraction analysis The crystal structure and purity of the synthesized CuO nanoparticles were determined by powder XRD. Fig.1 ISO 9001:2008 Certified Journal
|
Page 106