International Research Journal of Engineering and Technology (IRJET)
e-ISSN: 2395-0056
Volume: 04 Special Issue: 09 | Sep -2017
p-ISSN: 2395-0072
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One Day International Seminar on Materials Science & Technology (ISMST 2017) 4th August 2017 Organized by
Department of Physics, Mother Teresa Women’s University, Kodaikanal, Tamilnadu, India
SILVER NANO DOTS AS EFFECTIVE BIOSENSORS IN THE FIELD OF MEDICINE Ms .A.G.J.PATRICIA1, DR.UMADEVI MAHALINGAM2 1RESEARCH
SCHOLAR, DEPT.OF PHYSICS.MOTHER TERESA WOMEN’S UNIVERSITY.KODAIKANAL. MAHALINGAM.ASSOCIATE PROFESSOR .DEPT.OF PHYSICS.MOTHER TERESA WOMEN’S UNIVERSITY.KODAIKANAL. ---------------------------------------------------------------------***--------------------------------------------------------------------A target delivery in delicate human tissues. The silver Abstract – Silver nano dots have unique nanonano dots can be sensors for specific DNA sequences. dimensions that match the size of biological This can be achieved by mixing the targeted singlemolecules and structures so these nano dots can be stranded DNA with bio tinylated DNA fragments. used for in vivo and in vitro medical applications. Conventional nano sensors have side effects whereas 2. EXPERIMENTAL METHODS enhanced effectiveness and efficiency of silver nano dots finds wide array of applications as biosensors Uniform sized silver nano particles were for therapeutic purposes, disease diagnosis and fabricated through the reduction of silver ions by preventive health care .New biosensor applications fructose. In this process, a chemical solution was are emerging as smaller and smaller silver nano dots prepared by mixing .050 gms of fructose sugar in 50 are developed for molecular profiling and ml of de-ionized water taken in a conical flask. multiplexed biological assays. Minimal amount o f0.001 PVA was added to the above solution regularly and stirred by keeping the flask on Key Words: Silver nano dots, biosensors, bio cell a magnetic stirrer.Another solution of .0010 gms of targeting, drug delivery, diagnostics. silver nitrate was added to the above mixture drop by drop. This addition turned the solution to dark brown 1.INTRODUCTION within half an hour. Thus chemical reduction of silver nitrate has taken place. The solution was stable for A biosensor system consists of the following days together. components. 1)bioReceptor,2)transducers,3)detectors. The bio 2. RESULTS AND DISCUSSIONS receptor is a biological molecule like DNA strand, protein molecule, enzyme, antigen, antibody or cells The UV-VIS absorption spectra of the silver nano or tissues. The transducer transforms the recognition particle was examined. The absorption peak was event to a measurable electric signal which can be obtained at 446 nm. The XRD patterns were studied. used for the quantification of the analytic. The The diffraction peaks were observed at 2 theta values detector system receives the electrical signal which of 38.3,44.3 and 64.6 corresponding to the can be used from the transducer component and (111),(200) and (220) crystalline planes of cubic amplifies it suitably so that the corresponding silver. The XRD peaks were found to be broad in response can be read and studied properly, perform nature and this can be attributed to the nano size of more accurate forecasts and more robust studies of the particles. The average particle size has been by the situation of the critically ill patients and their estimated by using Debye-Scherrer formula. The survival rates. These silver nano dots are cross linked average particle size was found to be <20 nm. The to small ligands, peptides, aptamer , inhibitors that transmission electron microscopy analysis was done can bind with high specificity to many different to study the physical characteristics. Silver nano cellular receptors and targets. The nano dots are particles are formed with fairly even sized adapted to sense fat-soluble retinoids that is vitamin 2DR.M.UMADEVI
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