International Research Journal of Engineering and Technology (IRJET) e-ISSN: 2395-0056
Volume: 12 Issue: 03 | Mar 2025 www.irjet.net
Ayurveda Fundamental Principle “PRAKRUTI “, Vata, Pitta, Kapha, status measurement by Paper–chemical CHROMATOGRAPHY technique
Dr. Desh Bandhu Bajpai
Inventer and Chief Investigator, Ayurveda Clinical Pathology projects, Kanak Polytherapy Clinic and Research Center, 67/70, Bhusatoli Road, Bartan Bazar, Daulat Ganj, KANPUR – 208001 Uttar Pradesh, India
Abstract Prakruti has a great importance in Ayurveda medical science and Prakruti is considered to be the first and foremost base principle of Ayurveda. The Prakruti of a new born is fixed , just after coming out from mother’s womb. All earthly and environmental energies and fprces exposed in baby body at the time present and fixes this all with his her first inhalation of atmospheric air. With The start of respiration all functions of body starts including metabolic activities. According to Ayurveda, exposure of earthily energies and environmental properties fixes a stable properties of Vata, Pitta and Kapha ratio in the body which never changes in the whole life. The fixation of Prakruti and its determination is difficult to know. By paper CHROMATOGRAPHY Chemical technology, Prakruti of any individual can be determined. Ayurveda diagnosis, treatment and management is bases on the determination of Prakruti for exact , correct treatment planning. This Chromatography [9,10] technology will open the new doors for bew research gate in Ayurveda Diagnosis field. [5,6,8,8,9]
Keyword; Prakruti, Ayurveda Prakaruti, Ayurveda Principle Prakruti, Prakruti detection,, pRakruti detection and measurement, status quantification of prakruti
I. INTRODUCTION:- Prakruti Finding in Ayurveda
InAyurveda Prakrutiisan essential partofthescience. Ayurveda isbasedonSANKHYAPhilosophy,[2,3]where Prakruti and Purush is mainly considered. Vata, Pitta and Kapha are three basic cardinal principles on which, every action of Ayurvedaisbases.AllthesethreecardinalsfixPrakrutiatthetimeofjustbirth,whichremainsinsimilarfixedstatusratio and never changes in life time. Any changes in this ratio cause deviation of normal health and produces sickness.[5,9,10,11]
Fig-1;Justafterbirth,Panchmahabhutas propertiesmakesfromairandskyc9ombinationVATA,Fire cumHeat combinationPittaand WaterandEarthc0mbinationexposesrapidlyinbody
I.1 At the time of birth, fixation of Prakruti according to Ayurveda Philosophy
Earthly alongwithUniversalproperties,likeGravitation,Magnetism,soundsandultrasounds,Vibrationsandwaves,hot andcoldtemperature,Atmosphericpressure,
Air and envioronmental visible amd umvisible properties, Humidity, Water and earthly matters, beta gamma and unknownrays,Radiations andmany knownandunknownfactorsexistedinthisuniverse,theseallproperties exposes andassimilatein humanbodyjustafterbirthandseparatedfromumbilicalcord..AyurvedasaysthatVataisframedfrom thecombinationofAirand Sky,Pittaisacombination ofFire andHeat andKapha isacombinationof Waterand Earth. Just after the birth of child all earthly and universal properties energies exposes in to the child and just with the first respiration,allsensesactivatedofthebody.KeenobservationbyAyurvedaPractitionerscangivecluesofPrakrutiofan individual. Few Ayurveda masters has given detailed pictures, characteristics and features of the Vata Prakruti, Pitta PrakrutiandKaphaPrakrutimalesandfemalesmentionedintheirclassicalbooks.[5,6,7,8]
II.Assessment of Prakruti an essential requirement for effective Ayurveda management support in sickness
Prakruti assessment of an individual is very difficult to know. The only way is depicted by the Ayurveda experts after their log time consumed observations decries the body get-up, body built-up, face built, Hair colours, skin colours and textures, body specific characteristics, speech, walking and physical strength, mental behaviour, food habits, likes and dislikes, sexual behaviour and other features mentioned to determine. To get to know the Prakruti, a questionnaire is prepared and formatted based on these information’s lines, all characteristics mentioned in the classical books. After answeringtheformatquestions,ascrutinyofanswercangive cueofPrakrutiinsigleorincombination OncePrakrutiis fixedatthetimeofbirth,itisneverchangesinitsratio andwillremainsimilartilllife.ForAyurvedaDiagnosis,treatment andmanagementofany physicalandmentalailments,Prakrutidiagnosisisveryessentialinviewofeffectiveimpressive diseasemanagement.[1,2,34]
II.1 Chromatography Separation Test ; Individual Venous Blood required for PRAKRUT determination
ThisPrakrutitestisdevelopedinlaboratory.Asmallamountofun-centrifugedvenousbloodisrequiredforthistest.The fresh blood is used immediately after collection. Human Blood contains Fat, Protein, Carbohydrate, Minerals, Vitamin, Water, waste products and gases with other matters. When Blood, is mixed by reagents, chemicals, solvents, diluents, buffer solution etc. in a test tube, all reacts on the properties of blood and chemical changes takes place. Paper Chromatographyseparatedchemicalreactionchromeandcolourindifferentlayersofthebloodchemicalmixturesolution with their special colour identity. Vata, Pitta, Kapha and control produces their special color identity, which gives an amountoftheirpresenceinbodyaftermeasurement.
III. Methodology; Chromatography Test Requirements of Laboratory
Performingthistest,thelaboratoryshouldbewellequippedbythebelowgivenglassware’salongwithSolvents,diluents, Reagentsandothersupportingessentials.Thefollowinglistwillshowtheneedoflaboratoryforthistest.
1-Laboratoryspace withdarkroomandessentialequipments2-smalltesttubesofvarioussizes3-testtubestabs4speciallymadepaperholder sizeL 40mmx W14mmx H 8mm5-spritlevelmeter6-Glasssheet 8mmthickfor base7-UltravioletLight lamp8-Multi-Colourshowering Lamp9-Micro-puppetofvariousvolume10-Micropipette stand 11- White Filter paper stripe size L 24 mm x W 10 mm x H 1.3 mm 12- Paper cutter of best quality 13Reagents14-Solvents15-Diluents16-LaboratoryGlassware’s17-Watersources18-WorkingSpace19-UV&IRLight Sources 20- 2 ml Syriges 21- Blood collection Tube 22- E.D.T.A. Anti-coagulant chemical 23- Digital Microscope 24Measuring scales 25- Digital Venial Calliper 26- Graph papers 27- Lamination machine, Lamination Pouch 28- Room temperature and Humidity thermometer 29- other essential requirements 30- Graph papers 31- Scissors, paper cuttingtools32-HotAirdryer33-pHMeteR 34-MagnifyingGlassofvarioussizes [2]
Fig-2; Chro,atographyLaboratoryset-up;showingMicroscopes,micropipettes Colorimeter,pHmeter,inventedHolders,chemicalsandotheressentialitems
International Research Journal of Engineering and Technology (IRJET) e-ISSN: 2395-0056 Volume: 12 Issue: 03 | Mar 2025 www.irjet.net
IIII.2 Preparation before performing Chromatography Prakruti test
ThisChromatograph testisbasedontheindividualblood,whodesiresgorknowinghisindividualPRAKRUTI aiming at health purposes. Prakruti test is mainly bases on the hidden properties of the blood. Therefore for these analysis specialconditionsareimposedontheindividualforprocuringspecificmodifiesblood.Thespecificconditionforblood collectionsaregiven asfollowing;
1. Selectedindividualshouldhave fourdaysfast withouttakingnayfood,onlywatercanbetakenAfterfourdays,
onfifthdayinmorning,bloodshouldbetakenfortest
orif thisisnotpossibleforwishingindividualthensecondoptionsisgiven below
2. Sevendaysliveonfruitjuice,afterthisnext sevendayslive onfruitsandagain aftersevendayslive onmilkor buttermilk,aftercompletionofthreeweeks / 21daysof this kindof faston22nd daysbloodshouldbetaken in morningfortest
orif thisisnotpossibleforwishingindividualthenthird optionisgiven below
3. 60daysDugdh-kalporTakrakalpwithoutanysaltandmedication,after61daysbloodistaken orif thisisnotpossibleforwishingindividualthenfourth optionisgiven below
4. 120days liveonwithoutsaltandspicesmixed food with “Ayurveda Bloodpurifier remedies“ followed by strict vegetariandiet,on121daysbloodistakenfortest.
5-Aboveall,PANCHAKARNA,tne Ayurvedabody detoxification andcleaning classicalprossess with purvakarma andpashchatkarma,collectbloodafterpanchalarma
Abovementionedanyoneconditionsshouldfollowedby thewishing individual,whowanttoknowtheirPrakruti.If thewsw conditions are not followed and by mistake blood is tested, the test result will give DOSHA or TRIDOSHO statusandnotPRAKRUTI.
IIII.3 Chemical, Solvents Indicator, Diluents, Reagents regarding
Few solvents, diluents and reagents are prepared in our own established laboratory.[1] Specially designed Chromatography Paper-Holder with Specially designed Test Tubes are manufactured at our own established workshop. Other material used in this test has purchased from open market. This is a self financed project. This technologyisnotpatented;thereforeessentialinformationisbeingsharedinthisresearchpaper.
III.4 Mixing of Blood with chemical substances and pouring in Chromatography Holder
Chromatographyisasensitivetest,thereforeprecautionsshouldbetakenineverysteptoavoidanymistakes.Stepby stepactionisabestsolution,sothisshouldbefollowed.Beforestartingtestall chemicals,indicator,reagents,solvent, diluentsshouldkeepreadytousepositioninselves.Everythingwhichisinvolvedinthistestshouldbeinimmediate reachstage.Testtableshouldbecleanedanditssurfaceshouldbesmoothandplain,correctedbyspritlevel
Step-1; Dryemptyfourspeciallydesigned smalltesttubeistakenandputintesttubestands,marking Cforcontrol, VforVata,Pforpitta,KforKapha
Step-2;withthehelpofmicropipette40micromlbloodistakenandafterthis pourein theallfoursmalltesttubes.
Step-3;Equivalentquantityofsolvents,diluentsarepouredinallfourtesttubes
Step-4;CONTROLEtesttubeisseparatedandisnotmixedwithanyReagentsspeciallyfortestofVatapittaandKapha asNitrate,SodiumandPhosphateaccordingly.[1]
Step-5;Pourandmixadequatequantityof NitritereagentinVatatesttube
Step-6;Pourandmixadequatequantityof Sodium reagentin Pitta testtube
International Research Journal of Engineering and Technology (IRJET) e-ISSN: 2395-0056
Volume: 12 Issue: 03 | Mar 2025 www.irjet.net p-ISSN: 2395-0072
Step-7;Pourandmixadequatequantityof Phosphate reagentinKapha testtube
Alltesttubesmustshakegentlefor1-2minutes. Earliertoit,fixfilterpaperinsidethespeciallydesignedChromatographytestHolder MarkHolderC,V,P,Katthetail end. All test holder should keep in the flat position on a glass sheet properly balanced its surface by Sprit level. Any kindoftiltisavoided.
Pour carefully test tube mixture solution at the head side of the Holder including edge of filter paper, so that filter paperabsorbsliquidandspreaditatitsownabsorptionspeed.AnUltravioletlightshouldbeshoweredontheholder. Alllightsofthelaboratoryshouldbeclosedexceptultraviolet.
Fig-3; Measuringinstruments, DigitalVernierCaliper,Scaleandspeciallydesignedsimplemilliliterscales,for measuringco0lor-chromatolevelsize
Leave this all for 5 hours. Laboratory Room temperature should be noted with humidity. After 5 hours, all impregnated strips should out from holder and keep these all inside a warm chamber for drying well and settle chroma and color in between 30 c to 32 c degree temperature. After it, paste the strips safely and carefully on a graphpaper sheetwithglueandtransparenttape Whenwelldriedstripsobtained,fixthemonagraphpapermarked with the measurement in millimetres. Sticks all strip in 1 to 2 cm gap and after that laminate them by lamination machine in A4 size Every Graph paper should be marked C for Controle, V for Vata, P for Pitta and K for Kapha to avoidanymistake.[9,10]
Fig-4; DriedstripesofChromatograph,showingwithmeasuringscaleinmillimeter,Observecolor-ch4oma pigmentedonthestripanddifferentiateminutelyeachone. VatalevelisabovethanControlandPittaislowestand Kaphaisinmiddlelevel.InVataandKaphastripesthreelevelsareseen,whileinPittatwolevels.
IIII.4 Color Observation, Marking, and measuring of strips chroma and color levels on Laminated Graph Sheet
ChromatographedpaperStripsfixedonGraphpperinsequenceshouldbeevaluatedforPRAKRUTIdetection,withthe help of measuring tools. In these neasuring tools digital Vernier Caliper or Laser Distance Measuring meter are the bestbecausebothhmeasures upto2digitsafterdecimal.
Appropriate markings of chroma pigmented level’s impressed lines on paper should be done in millimetres by segregatimg eachandeverylinesindifferentcolorsections.NotedowmeveryreadingofControle,Vata,Pitta,Kapha stripes.MarklinesandwritesnomenclatureonLaminatedgraph papersheetforclarity.
International Research Journal of Engineering and Technology (IRJET) e-ISSN: 2395-0056
Volume: 12 Issue: 03 | Mar 2025 www.irjet.net p-ISSN: 2395-0072
Fig-5;ChromatographPaperstripesarrangedinsequenceonGraphpaper. Afterstickingwithglueandtransparent tape,laminatethegraphsheetwithlaminationafteressentialmarkings.Afterthat,allstripesshouldbemeasuredby scaleinlayerwise.
InChromatography,keen observationpfcolors aremostimportantantand essential.Nakedeyes, useofmagnifying Glass and Digital Microscopes and color showering lamps are helpful for better cokor recogmition purpose. A Dark roomreadingunderultraviolet andInfraredlight providesbestreadimgs.
IIII.5 Interpretation and report of chromatography examination with final results
After measurement of all strip’s indicated level values, interpretation is done on the basis of these obtained values. Measuringisanimportantprtofthetest.Chromatographytestprovidesfewcolours
1- In General, beginning part of chroma is seen almost short in length compare to others due to containing heavy molecules of blood Proteins, Carbohydrates and Fats, which inhibits the progress speed of test- liquide inside the paperholder.Uptoacertainlevelthisheavymaterialmoleculesinhibitstheirprogress,afterthatthenextlightliquid molecules come forward with its thinner capacity and thus second and more level lines in progresses. Finally chromatographcompletesaftersoakingallliquids.
2-Fluidissoftthanearlierlevel,thereforetheremainsoft fluidascends uptosecondandmore level.Itstaysitsown progressuptoitsthinnessfollowedbynext.
3- The third level fluid is much thinner than others. Therefore it s[reads up to its maximums height, bases on thinness.Thisisthelaststageprogressof theentiretestfluid.
Fig-6; Chromatographystatusprinted Reportprepare aftermeasuringandcollectionofdata,feudedin Excel programming.DatavaluesandChartshowsclarityofeachfeededvalues.
The colors of the fluid occurs due to Blood cells, melanin, reaction of reagents, solvents, diluents, indicator chemical actions on blood containing protein, carbohydrate, fat, minerals, vitamin and serum properties. Under microscopic examinationofstrips,redbloodcellsareseeninbaseor1st part,insecondlevellightred,bron,pink,green,yelloware seen. In third level segnent of strip white, light green, light yellow color is seen. The measured values should be
International Research Journal of Engineering and Technology (IRJET) e-ISSN: 2395-0056
Volume: 12 Issue: 03 | Mar 2025 www.irjet.net p-ISSN: 2395-0072
noted and transfer in report formate, especially programmed for this purpose. After feeding all data in formate , a printablereport is obtained.[9,10]
IIV. CONCLUSION
In this research paper, Chromatographic teqnique is uded for status measurement of Ayurveda PRAKRUTI, for which no other ways to obtain the level in the body. The only way is the description of body features, body get-up, habits and intellectual amd physical body idenyification of body built and fuctions. Recognition of these features are not easy to access andmayconfuse due tounknown phy siological internal effects. ChromatographicanalysisisbasedontheBlood and its chemical constituents which reacts with the identified chemicals and reagents, buffer solutions, diluents interreaction, which reflects changes in the entire solutions and Blood chemistry. Thus newly invented paper chromatographygives chemicalsreactionschroma levels.Afteranalysingthesechromalevelsresultscanbeinterpreted. ThusPRAKRUTIcanbeassessedeasilywithperfectiomand certainity.
ACKNOWLEDEGEMENT
REFERENCE
1. Bajpai Dr. Desh Bandhu , research paper published in International Research Journal of Engineering and Technology,volume11,issue,July7,2024,pages-12-22, title“VenousWholeBloodTestforQuantizationofthe standard of Ayurveda principle presence in human body including disease diagnosis invented technology developedinlaboratory“
2. Bajpai Dr. Desh Bandhu , research paper published title “Status quantification of Ayurveda Fundamentals in Human Body b7y Newly Invented “Ayurveda Tridosho Scanner” diagnosis machine Technology with Artificial Intelligence for acquiring data“ in International Research Journal of Engineering and Technology, volume 11, issue,June6,2024,pages-212-221
3. BajpaiDr.DeshBandhu,researchpaperpublishedtitle AyushTherapiescumAllopathysupportedHomoeostatic Integrated comprehensive diagnosis and treatment and management for Sadhya, Kastsadhya, Asadhya, Yapya, Chronic, Lifelong, Incurable disease condition in International Research Journal of Engineering and Technology, volume11,issue,October10,2024,pages-128-136
4. Bajpai Dr. Desh Bandhu , research paper published title “Ayurveda Head-Torso-Limbs Whole-body Scanner with artificial intelligence innovative technology for homoeostasis data-collection of Ayurveda fundamental including human organs internal health anomalies “ in International Research Journal of Engineering and Technology, volume11,issue,
5. Sharangadhar,composedby;Sharagdharsamhitaclassicalbook,relevantchapters
6. MadhavNidanclassicalbook,relevantchapters
7. BhavPrakash,classicalbook,relevantchapters
8. Haridaswrittenbook“ChikitsaChandrodaya”
9. A.BraithwaiteandF.J.Smith, writtenbook “Chromatographic Methods”,fifthedition1996,relevantchapters
10. AhujaSatinder,writtenbook“ChromatographyandSeparationScience”, Volume-4,2003, relevantchapters
11. H.N.Katurewrittenbook,PanchakarmapublishedbyBaidyabathPrajashan,Jhansi,IP
International Research Journal of Engineering and Technology (IRJET) e-ISSN: 2395-0056
Volume: 12 Issue: 03 | Mar 2025 www.irjet.net p-ISSN: 2395-0072
BIBLIOGRAPHY:
Dr. Desh Bandhu Bajpai, Chief Investigator, [1] Electro Tridosho Graphy/ Gram, E.T.G. AyurvedaScan and Ayurveda Tridosho Scanner and Ayurveda Head-Trunk-Limbs Whole Body Scanner [A.H.T.L.W.B.S.] and Ayurveda Blood Chemical ChemistryAnalyserTechnology.
Born 20.11.1945 at village Karnaipur, situated at Raibareilly- Lal Kuan- Unnao Road, Police station Bara Sagavar, Post officeBaraSagvar,DisttUnnao,UttarPradesh,Bharat.Primeryeducation at Madarsa,Karnaipur,afterthatNagarPalika Munispality Basic School, Kanpur.High School and Intermediate from Board of High School and Intermediate education, Allahabad. Graduate in Homoeopathy, Bachelor of medicine and surgery [BMS,] from Homoeopathic medicine Board, Lucknow,UttarPardeshinyear1972.AyurvedaandUnanigraduatefromNikhilBhartvarshiyaAyurvedaVidyapith,New Delhias Ayurvedacharya[B.A.M.S.]inyear1977.UttarPradeshGovernmentallowedAyurvedicPhysiciansforusingover Allopathic 45 alkaloids based remedies. Integrated diagnosis and treatment studied in Munich, Germany at Hospital for NaturecuremethodsandgotDiplomainPhytotherapyandHomoeopathyinyear1977-1980.PostGraduateinAyurveda, DoctorofMedicine[M.D.]andHomoeopathyM.I.C.R.withCardio-vascularcarecertificatesetcetc.PracticinginAyurveda HomoeopathicUnaniAllopathyphysiotherpuAcupuncturesilultanoeouslyatourresearchcenteratKanpurUttarPradesh over60+years.