The source is allantoic fluid of 10-12 days old embryonated chicken eggs, inoculated with the appropriate influenza A strain. Purified viruses are inactivated with thimerosal and beta propiolactone treatment. Purity of all products is >90% and these antigens can be used for detection of antibodies to influenza A viruses in ELISA, HIT and Western blotting. Influenza A (H1N1) antigens do not have cross-reactivity in ELISA with panel of MAbs to HA of heterological subtype of influenza A (H3N2) viruses, and Influenza A (H3N2) antigens do not have cross-reactivity in ELISA with panel of MAbs to HA of heterological subtype of influenza A (H1N1) viruses. Also these antigens are not cross-reacting with MAbs to HA of influenza B virus, MAbs to NP of influenza B virus and in hemagglutination inhibition test with antisera to different subtypes of influenza A and B viruses (See Table 1.). Table 1. Control investigation of influenza A antigens in hemagglutination inhibition test. Virus: A/Panama/2007/99 (H3N2) B/Tokio/53/99 A/New Caledonia/20/99 (H1N1) A/swine/1976/31 (Hsw1N1) A/St.Petersburg/186/00 (H3N2) A/Singapore/1/57 (H2N2) Antibody titers in strain specific rabbit sera to: Influenza A virus Influenza B virus Ssw1 SH3 SH2 SB1 <10 320 <10 <10 <10 <10 <10 320 <10 <10 <10 <10 160 <10 <10 <10 <10 320 <10 <10 <10 <10 320 <10 SH1 <10 <10 640 <10 <10 <10 SH1: antiserum to strain A/New Caledonia/20/99 (H1N1) Ssw1: antiserum to strain A/swine/1976/31 (Hsw1N1) SH3: antiserum to strain A/St.Petersburg/186/00 (H3N2) 1,4 + + + 1,2 SH2: antiserum to strain A/ Singapore/1/57 (H2N2) SB1: antiserum to strain B/Tokio/53/99 MAb 3B3 2 MAb 1C6 1,8 MAb 4H7 + 0,8 0,6 OD 450 OD 450 1 + + + MAb 12/5 1,6 MAb 1C6 1,4 + MAb 4H7 + MAb 3B3 1,2 1 0,8 0,6 0,4 + 0,4 5000 500 + + 0,5 + + 5 + + + 50 0 + 500 0,2 + + 5000 + + + 0 + 0,2 50 5 0,5 Concentration of MAbs (ng/ml) Concentration of MAbs (ng/ml) Figure 1. Control of specific activity and cross-reactivity of influenza A/New Caledonia/20/99 virus in ELISA with monoclonal antibodies to different influenza viruses. MAb 3B3 to HA of influenza A/Beijing/262/95 (H1N1) virus MAb 1C6 to NP of influenza A/chick/Pennsylvania/1370/83 (H5N1) virus MAb 4H7 to HA of influenza B/Panama/45/90 virus Figure 2. Control of specific activity and cross-reactivity of influenza A/Panama/2007/99 virus in ELISA with monoclonal antibodies to different influenza viruses. MAb 12/5 to HA of influenza A/Panama/2007/99 (H3N2) virus MAb 1C6 to NP of influenza A/chick/Pennsylvania/1370/83 (H5N1) virus MAb 4H7 to HA of influenza B/Panama/45/90 virus MAb 3B3 to HA of influenza A/Beijing/262/95 (H1N1) virus Ordering information: Product Cat. # Strain Remarks 8IN73 8IN73-2 8IN73-3 8IN73-4 8IN74 8IN74-1 8IN74-2 8IN74-3 8IN74-4 A/Taiwan/1/86 A/Beijing/262/95 A/New Caledonia/20/99 A/Solomon Islands/03/06 A/Shangdong/9/93 A/Panama/2007/99 A/Kiev/301/94 A/Wisconsin/67/05 A/Brisbane/10/07 EIA, HIT, WB EIA, HIT, WB EIA, HIT, WB EIA, HIT, WB EIA, HIT, WB EIA, HIT, WB EIA, HIT, WB EIA, HIT, WB EIA, HIT, WB Influenza A (H1N1) virus Influenza A (H1N1) virus-2 Influenza A (H1N1) virus-3 Influenza A (H1N1) virus-4 Influenza A (H3N2) virus Influenza A (H3N2) virus-1 Influenza A (H3N2) virus-2 Influenza A (H3N2) virus-3 Influenza A (H3N2) virus-4 8 INFECTIOUS DISEASE REAGENTS