ISSN 2348-313X (Print) International Journal of Life Sciences Research ISSN 2348-3148 (online) Vol. 9, Issue 1, pp: (37-42), Month: January - March 2021, Available at: www.researchpublish.com
Relevance of zinc concentration in seminal plasma with sperm morphology in infertile males Anuradha H Pawar1, Sanghya Singh Bais2 Lecturer Department of Physiology1 , Lecturer Department of Biochemistry 2 SDK Dental college, Nagpur,India
Abstract: Zinc is an essential micronutrient for spermatogenesis and normal sperm function. There have been conflicting reports on the relation of seminal zinc on various seminal parameters. This study aimed to evaluate zinc levels in seminal plasma of infertile men and its relation to infertility. Objective: To find out relation of zinc concentration in seminal plasma with sperm morphology in infertile subjects having normozoospermia, oligozoospermia and azoospermia. Design: Cross sectional study Setting: This study is carried out in the reproductive biology unit in department of Physiology &Central Research Lab, Jawaharlal Nehru Medical College Sawangi Wardha (MS) from June 2009 to Dec 2011. Subjects and method: Study involved detail history taking; clinical examination followed by detailed semen analysis as per the WHO criteria. The seminal plasma was then classified in to six groups patients having normozoospermia (40), oligozoospermia (16) ,ashanospermia withnormal count (9),oligoasthenospermia (20),teratozoospermia(10) and azoospermia (10). They were used for zinc determination by zinc kit (supplied by Coral pharmaceuticals) using colorimeter. The morphology of the sperm is also evaluated. With WHO criteria, sample is normal if 30 % or more of the observed sperm have normal morphology.Slides for morphological smear were prepared and smear were fixed and stained with papanicolau stain, the procedure was done according to WHO manual. Results: Zinc levels were lower among azoospermic & oligospermic as compared to normozoospermic patients. Seminal plasma zinc showed a positive correlation with normal spermatozoa morphology. Conclusion: Zinc level in seminal plasma and its relationship to seminal parameters might give useful information regarding male reproductive dysfunction. It seems that the estimation of seminal plasma zinc may help in investigation and treatment of infertile subjects. Keywords: Normozospermia, oligospermia, oligoasthenospermia, taratozoospermia, asthenospermia, azoospermia.
I. INTRODUCTION What makes the earth a unique planet is entity called life. Reproduction is necessary for progress of species. With advance of civilization sterility has become more than eternal, a medical problem, a problem which closely affects the welfare of the society. Besides, the riddle of population explosion there are challenges to infertility as there is alarming increase in cases of infertility. Infertility is defined as failure of a couple to achieve pregnancy after „a‟ year of unprotected intercourse with the same partner. Semen is the fluid that a man ejaculates. The whole semen is the suspension of spermatozoa or sperms in the fluid medium called seminal plasma. The main portion of semen originates from three glandular organs. The constituents
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