ISSN 2348-313X (Print) International Journal of Life Sciences Research ISSN 2348-3148 (online) Vol. 9, Issue 1, pp: (16-21), Month: January - March 2021, Available at: www.researchpublish.com
ANTI-ULCER ACTIVITY OF ADHATODA VASICA EXTRACT ON GAMMA RADIATION INDUCED GASTRIC LESIONS IN MICE ANUPAM PUNDIR1 AND SUSHMA SHARMA2 Ph.D. Scholar, Department of Biosciences, Himachal Pradesh University, Summerhill, Shimla-171005, India Email: bhardwaj.anu08@gmail.com. Professor, Department of Biosciences, Himachal Pradesh University, Summerhill, Shimla-171005, India Email: sushma_bio_sci@rediffmail.com.
Abstract: The role of radiation biology has acquired greater relevance and significance in addressing the health issues. Radiation is often used to treat malignant tumours, or in combination with surgery. Despite the advantage of radiotherapy and improvements in techniques, many patients experience moderate to severe side effects. Adhatoda vasica, an evergreen, gregarious, stiff and perennial shrub of family Acanthaceae has been used as herbal medicine in treating a wide variety of diseases in India. The objective of the present study was to evaluate the anti-ulcer effect of Adhatoda vasica extract on gamma –radiation induced gastric lesions in mice. Mice showing no signs of morbidity were divided into four groups (i) control (ii) Adhatoda extract treated mice (900mg/kg body weight) (iii) Irradiated mice (5gy) (iv) Adhatoda extract treated and irradiated mice. Adhatoda extract was administered orally to the animals (900mg/kg body weight) for a period of 30 days. After 10 days of extract administration mice were irradiated with 5Gy γ-rays. Mice were sacrificed by cervical dislocation and stomach was excised on 10, 20 and 30 days. Stomach was opened along the greater curvature. It was rinsed with water to remove gastric contents and blood clots. Stomach was then examined by a 10×magnifier lens for the assessment of ulcers. Control group and Adhatoda extract treated mice stomach revealed normal and healthy gastric architecture. Irradiated group of mice showed multiple haemmoragic spots and erosions during different stages of investigation. The mice group pretreated with extract and then irradiated showed significant ulceration inhibition providing protection to stomach. Keywords: Gamma radiaton, Anti-ulcer effect, Hemorrhagic spots, Ulceration inhibition.
1. INTRODUCTION In the era of nuclear energy, it is important to understand the basic mechanisms of radiation interaction with living matter. Ionizing radiations are widely used for the treatment of cancer. However, one of the limitations for using radiation is its toxic effects on normal tissues. Radiation induced damage to normal tissues can be partially reduced by the use of radioprotectors that scavenge free radicals produced during irradiation, sparing cancer tissues [1] . Natural products may be beneficial in protecting against the radiation-induced damage, as they are less toxic or practically non-toxic compared to the synthetic compounds at their optimum protective dose levels [2]. The exposure of animals to a single whole-body dose of ionizing radiation results in a complex set of symptoms whose onset, nature and severity depends on both total radiation dose and radiation quality. Radiation symptoms are mainly classified into three syndromes haematopoietic syndrome, the gastrointestinal syndrome and the central nervous system syndrome [3]. Therefore, the interest has been
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