International Journal of Healthcare Sciences ISSN 2348-5728 (Online) Vol. 8, Issue 2, pp: (186-196), Month: October 2020 - March 2021, Available at: www.researchpublish.com
Cytokines levels as biomarkers for the diagnosis of latent tuberculosis among Sudanese patients with poorly controlled diabetic mellitus type Ⅱ, SUDAN 2019 1
Wail Mohamed Alkhraj, 2Prof. Ahmed Kamal Bolad, 3Prof. Atif. Elagib, 4 Dr. Fawzi Abdulrahim Mahgoub 1
Ribat Hospital University,
Al Neelain University, Khartoum-Sudan 2
Faculty of medicine, Al Neelain University
3
National Center For Research, Khartoum-Sudan
4
University of najran/Sharough general hospital,KSA.
Abstract: Pulmonary tuberculosis is the one of the main health problem among Sudanese diabetic patients who are considered as immune-compromised patients. Objective of the study:- To evaluate cytokines IL6,IL10 and TNFα among study groups (diabetic and tuberculosis patients , non diabetic tuberculosis patients, diabetic and cardio vascular disease patients and control group of healthy individuals). Methodology :- This study was designed as descriptive a prospective, analytical case-control study .this study was enrolled 402 individuals ,40 were diabetic and tuberculosis patients, 41 non diabetic tuberculosis, diabetic CVD were 160 and 161 healthy control. Five ml of blood withdraw in plain vaccutainor after informed consent from individuals whom participate in this study. Estimation of the cytokines(IL6,IL10 and TNFα) levels from all participate were done by using direct ELISA technique. Results:- The mean levels of IL6 was recorded in 161 healthy persons 94.80pg/ml. while recorded 196.27pg/ml in 41 tuberculosis non diabetic patients, 210.27pg/ml in 40 tuberculosis diabetic patients and 7.90pg/ml in 160 patients tuberculosis diabetic with CVD 199.33pg/ml. The mean levels of IL10 was recorded in 161 healthy persons mean 4.21pg/ml, while mean 8.84pg/ml in 41 TB non diabetic, mean 8.61pg/ml in 40 TB diabetic patients and 7.90pg/ml in 160 TB diabetic with CVD patients. The mean levels of TNFα was 81.59pg/ml in 161 healthy persons, while it was 232.29pg/ml in 40 TB non diabetic patients, 261.78pg/ml in 40 TB diabetic patients and 297.42pg/ml in 160 TB diabetic with CVD. Conclusion:- Serum levels of IL6,IL10 and TNF alpha increased in patients with no signs and symptoms of tuberculosis (latent phase). The cut off values 68pg/ml ;5.58pg/ml;279.42pg/ml for IL6, IL10 and TNF alpha respectively are diagnostic for latent tuberculosis Where was no relationship between gender and IL6, IL10 and TNF alpha levels. Recommendation:- All patients with complicated diabetic should be test for the latent tuberculosis in poorly controlled diabetic mellitus type Ⅱ. Keywords: Pulmonary tuberculosis, Sudanese diabetic patients, healthy control.
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