ISSN 2348-313X (Print) International Journal of Life Sciences Research ISSN 2348-3148 (online) Vol. 8, Issue 4, pp: (70-78), Month: October - December 2020, Available at: www.researchpublish.com
Wound Healing Efficacy of Chicken Feet Gelatin and alginate Composite Films Blends with Black Turmeric (Curcuma Caesia Roxb.,) Extract S. Seethalakshmi1, V. Ramnath2, R. Anitha3, J.John Robert4, A.Arun5 1 2
Assistant Professor, Department of Biochemistry, St.Joseph’s College of Arts and Science, Cuddalore.
Associate Professor, Department of Biochemistry, Thiruvalluvar Arts and Science College, Kurinjipadi.
3,4
Assistant Professor, Department of Biochemistry, St.Joseph’s College of Arts and Science, Cuddalore.
Abstract: The present study of this work is to prepare a novel wound dressing composites are enriched with medicinal values which prevents the microbial contaminations and cell damage to the wounded surface. So that the films consist of antioxidant and antimicrobial properties are an appreciable one for the composite film. In this studies, gelatin is a proteinous substance isolated from the chicken feet along with sodium alginate, the composite films are prepared and these films are enriched with ethanolic extract of black turmeric (Curcuma Caesia Roxb ). The composite films are prepared and characterised for FTIR, SEM, mechanical strength, water absorption studies and also evaluate the invitro studies such as antioxidant and antimicrobial activities. As the result, shows that the G-ALG-CC increased water absorption capacity and better mechanical properties of film in contrast to GALG. The SEM and FTIR results exhibit the natural features of the composite film. The G-ALG-CC composite film possess significantly increased levels of antioxidant and antimicrobial activity. The Scanning Electron Microscope images of G-ALG-CC reveals the smooth surface of the film which is necessary requirement of the biomaterial. Keywords: Gelatin, alginate, black turmeric, curcuma caseia, composite film.
1. INTRODUCTION Many kinds of biomaterials are used to treat various diseases especially wound healing. Several components are exist as naturally in the skin and also actively participated in the wound healing process. Due to their natural biological features of biodegradability and biocompatibility of these components are favourably approached for various therapeutic purposes [1]. There is very much need to develop a novel biodegradable material, which is widely used in the field of skin tissue engineering and wound healing material due to the their high demand for skin repair and skin replacement therapies. An ideal film should exhibit certain behaviour such as diminished storage requirement, increased shelf life, biodegradability and biocompatibility [2]. Gelatin is an excellent biopolymer which is obtained from the poultry byproducts such as bone, feet, cartilage and skin [3]. It is an edible proteinous product derived from the denatured collagen [4]. The native conformation of the collagen is deformed due to heating and then regaining their normal state while cooling process. The amino acid composition of gelatin is more or less similar to collagen with some modification occurs due to their extraction process [5]. It is rich in proline, hydroxyproline, alanine and glycine, but varying in distinct ratios and combinations [6]. The promising physical features of Gelatins are translucent, colorless, tasteless, odorless, high mechanical strength, low melting point, elasticity, and increased shelf- life [7]. The peculiar features of gelatin have an ability to form thermoreversible gels and highly water soluble that meets the body temperature [8]. The nature of the gelatin are biodegradable, biocompatible and nontoxic which is suitable for the physiological conditions [9]. Gelatins are categorized into two types based on their
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