ISSN 2348-1218 (print) International Journal of Interdisciplinary Research and Innovations ISSN 2348-1226 (online) Vol. 8, Issue 4, pp: (126-131), Month: October - December 2020, Available at: www.researchpublish.com
IN VITRO GROWTH RESPONSE OF EXPLANTS AND MICROPROPAGATION OF Brassica oleraceae L. var Capitata Daud, N. F.1*, Hasbullah, N. A.1, Azis, N.A.1, Rasad, F.M.1, Lassim, M.M2 1
2
Department of Agricultural Science, Faculty of Technical and Vocational, Universiti Pendidikan Sultan Idris (UPSI), 35900 Tanjung Malim, Perak, Malaysia
Faculty of Sustainable Agriculture (Sandakan Campus), Universiti Malaysia Sabah (UMS), 90000, Sandakan, Sabah, Malaysia. Email: fare_inmush258@yahoo.com
Abstract: Experiments were carried out to establish optimum culture condition and to identify the most responsive explants for regeneration of Brassica oleraceae L. var Capitata through plant tissue culture system. The experiment was induced by manipulating various growth regulators during organogenesis using stems, petioles, leaves and roots as explants. The experimental design of this study was Complete Randomized Design (CRD) method. For shoot regeneration using different concentrations of BAP and NAA, the most responsive explant was stem explants in which MS medium supplemented with 1.5 mg/L BAP and 0.5 mg/L NAA produced 8.400 ± 0.889 shoots per explant. Whereas, for root regeneration using different concentrations of BAP and NAA, the most responsive explant was leaf in which MS media supplemented with 0.5 mg/L BAP and 0.5 mg/L NAA produced 51.133 ± 2.929 roots per explant. This research showed that a morphogenesis study of Brassica oleraceae L. var Capitata through tissue culture system was successfully achieved. This study could lead to the better research and development (R&D) for vegetables in the future. Keywords: morphogenesis, in vitro, Murashige and Skoog medium, callus, plantlets.
I. INTRODUCTION Tissue culture is one of the methods to propagate plants in a short period of time. It is also known as in vitro propagation and micropropagation. Tissue culture is one of the methods in which small part of plants (explant) whether the leaves, stems (organ) or cells were placed into the cultured medium for growth purpose in sterile or aseptic that is in the condition that free from microorganisms such as fungus, bacteria, and viruses [1]. Besides that, plant tissue culture or micropropagation means the way of rapidly multiplying plant materials stock to ensure large number of plant progeny could be produced [2]. Plant tissue culture needs the medium to replace the role of soil and fertilizer. There are many types of plant tissue culture medium. The most commonly used is known as Murashige and Skoog medium (MS) [3]. Brassica oleraceae L. var Capitata or commonly known as cabbage is derived from Brassica family. Cabbage is an important vegetable plant; grown on large surfaces worldwide, occupying the third place in the world, after solanaceae and onion [4]. It contains high amounts of vitamin such as vitamin K, C, A and folic acid, flavonoids, fiber, proteins and mineral anticarcinogenic and glucosinolates [5]. For instance, antioxidant is rich in the cabbage can be used to treat many types of cancer. The risk of breast and colon cancer will be lowered by eating cabbage two to three times per week [6]. Due to its importance, Brassica oleraceae L. var Capitata has very high demand by consumers. The sustainability of
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