ISSN 2348-1218 (print) ISSN 2348-1226 (online) Vol. 10, Issue 1, pp: (1-5), Month: January - March 2022, Available at: www.researchpublish.com
International Journal of Interdisciplinary Research and Innovations
Establishment of in vitro Micropropagation from Explants of Punica granatum L. Muhammad Al-Amin Mazlan, Nor Azlina Hasbullah1, Siti Zubaidah Lood, Fadhlul Khaliq Ab Patah 1 1
Email: azlina.h@ftv.upsi.edu.my
Department of Agricultural Science, Faculty of Technical and Vocational (FTV), Universiti Pendidikan Sultan Idris (UPSI), 35900 Tanjong Malim, Perak, Malaysia
Abstract: The purpose of this study was to examine the ability of in vitro propagation of Punica granatum L. through tissue culture system. The experimental design that was used in this research is Completely Randomized Design (CRD). Cultures were stored at a temperature of 25 ± 1ºC and light 16 hours of light, 8 hours dark. All data was recorded and analyzed using ANNOVA. Leaf explants were found to be very responsive when cultured on MS medium supplemented with plant growth regulators at concentration of 2.0 mg/L Benzylaminopurine (BAP) + 1.5 mg/L Napthalene Acetic Acid (NAA) and has been identified as the optimum medium for shoot regeneration. Leaf explant produced 1.33±0.32 shoots per explant at week 8. Whereas from stem explant, Murashige and Skoog (MS) medium added with 0.5 mg/L BAP and 2.0 mg/L NAA gave the highest shoot development with 1.00 ± 0.28 shoots per explant Various combinations of auxin such as NAA and cytokinin such as BAP, at 1.5 mg/L was used for a complete regeneration of Punica granatum L. in vitro. In conclusion, in vitro micropropagation technique is a suitable alternative in producing new generation of Punica granatum L. and its features are preserved. It proves that plant tissue culture technology could be an alternative solution to achieve high quality of Punica granatum L., therefore could increase the crop production. Keywords: Punica granatum L., tissue culture, micropropagation, explants, MS medium, growth regulators.
I. INTRODUCTION Micropropagation is the art and science of plant multiplication in vitro. The procedure of micropropagation incorporates many strides, for example; stock plant care, explant selection and sanitization, media control to get expansion, establishment acclimation, and development of the liners. Other than that, micropropagation is the act of quickly increasing the stock plant material to deliver countless plants, utilizing present day plant tissue culture strategies. Micropropagation is utilized to multiply new plants, for example; those that have been hereditarily changed or reproduced through ordinary plant rearing strategies. It is additionally used to give an adequate number of plantlets for planting from a stock plant which does not deliver seeds, or does not react well to vegetative multiplication. The common propagation method of Punica granatum L. was by seed. However, germination by seeds shows a high degree of variability because of cross-pollinated nature of the plant. Other than that, the seeds of Punica granatum L. take time to germinate by using traditional method. This is because the seeds have hard seed coat, therefore, longer time to break the seed coat. Thus, it is not attractive approach for producing a large number of elite plants within a short period of time. Besides, the percentage of seed germination through conventional method is lower than through culture tissue technique. The demand of pomegranate is increasing due to its taste and medicinal properties. It is conventionally propagated by air layering and cuttings [1], [2] and [3]. In the present study, in vitro germination and regeneration of Punica granatum L. was conducted. The effects of plant growth regulators on in vitro micropropagation of explants of Punica granatum L. was observed. This study would help in overcoming the problem in propagation of pomegranate through conventional methods. A number of studies have been conducted on micropropagation of pomegranate by previous researchers [4] and [5] by using various types of explants including leaf, cotyledon, shoot tips and nodal explants.
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