ISSN 2348-313X (Print) International Journal of Life Sciences Research ISSN 2348-3148 (online) Vol. 8, Issue 1, pp: (1-4), Month: January - March 2020, Available at: www.researchpublish.com
Characterization of lipid producing fungi isolated from soils K C Naveena1, Ramalingappa B2 1, 2
Department of Microbiology, Davangere University, Davangere-577007, Karnataka, India. *Corresponding author. E-mail: ramalingappa.88@gmail.com
Abstract: Microbial lipids are in the form of triacylglycerols (TAGs). Glucose was used as carbon source for accumulation of triacylglycerols. In this study, isolated fungi were screened for their lipid producing capacities by Sudan Black B staining method. Biochemical and molecular characterizations were done for identifications of potential lipid producers. The fungus Epicaleosporium ramularioids and Podosphaera xanthii showed its lipid productivity 24.2736% and 45.67% respectively. The extracted lipids were analyzed by Gas chromatography with mass spectroscopy for fatty acid determination. Keywords: Lipids, Oleaginous, Sudan Black B stain, GC-MS.
1. INTRODUCTION Microorganisms can accumulate lipids more than 20% of their dry weight is called oleaginous. The obtained lipids are considered as raw material for production of biodiesel (Ratledge and Wynn, 2002). Subsequently, the production of biofuels like bioethanol and biodiesel has expanded worldwide for more than 10 years. Biodiesel, which is gotten from vegetable and animal fats, is for the most part a blend of unsaturated fat methyl esters (FAMEs). Utilization of biodiesel has been expanding quickly as an alternative source of energy (Meng et al., 2009). Nowadays, the exploration of oil producing microorganisms primarily center around the improvement of fermentative states of the current strain (Li et al., 2007) as well as screening functional oil producing microorganisms (Patnayak and sree, 2005). However, very few studies had been directly on isolating microorganisms from natural environment, which convert substrate to biodiesel. 20 strains isolated from oil rich soil and nut samples, which belong to 13 different species of fat yeast. This indicates there is abundant oil producing microorganisms in nature environment (Pan et al., 2009). Therefore, fat producing microorganisms are screening from natural environment, on one hand, it will expand the resources of oil microorganism sp., on the other hand, it also possibly obtains oil microorganism that have many industrial applications.
2. MATERIALS AND METHODS Isolation of lipid producing fungi The soil samples were obtained for the isolation of microorganisms. Upon collection of soil samples were allowed to refrigerator condition at 4о C for further use. For isolation 0.1 ml of diluted soil suspension spread on yeast peptone dextrose agar medium (YPD) having the pH 5.5 and 6.5, the inoculated plates were kept for incubation at lab temperature for 3 days. Screening of potential lipid producers The oleaginous fungus was stained with qualitative analysis of Sudan Black B staining method and observes under a microscope for presence of intracellular lipids (Thakur, 1989).
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