International Journal of Healthcare Sciences ISSN 2348-5728 (Online) Vol. 10, Issue 1, pp: (251-257), Month: April 2022 - September 2022, Available at: www.researchpublish.com
Assisted Oocyte Activation Method (Ca ACTIVATION) Improves Fertilization in Patients with Recurrent Fertilization Failure Ayman S. El-Dorghamy1, Bahgat A. El-Fiky2, Mohammed H. Sayed Ahmad2 1-Department of Environmental Biotechnology, Genetic Engineering and Biotechnology Research Institute, Sadat City University. 2-Department of Animal Biotechnology, Genetic Engineering and Biotechnology Research Institute, Sadat City University. DOI: https://doi.org/10.5281/zenodo.7034452
Published Date: 30-August-2022
Abstract: Total fertilization failure (TFF) happens in 1–3% of total intracytoplasmic sperm injection (ICSI) cycles and may reoccur in sequent cycles. Regardless the high success rate with the applying of intracytoplasmic sperm injection (ICSI) , there's still a little number of couples who cannot get fertilized eggs after using ICSI . Six couples experiencing recurrent TFF or low fertilization rate (<10%) after ICSI were recorded in this study. Compared with the regular ICSI group and the conventional ICSI-AOA group, the new AOA method using calcium ionophore, can seriously increase the fertilization rate from less than 10% up to approximately 50% in most cases. The normal distribution of sperm-related oocyte activation factor phospholipase C zeta (PLCz1) in the sperms of the cases indicated the absence of an aberrant Ca signaling activation. AOA method using calcium (Calcium ionophore A23187)was able to effectively improve the fertilization rate in the majority of patients suffering from TFF. This AOA method had a potential therapeutic effect on those couples experiencing TFF, even after intracytoplasmic sperm injection(ICSI), which may overcome the severe fertilization deficiencies in patients with a repeated low fertilization or TFF. Keywords: total fertilization failure, Calcium ionophore A23187, fertilization, assisted oocyte activation.
I. INTRODUCTION Assisted reproductive techniques (ART) provide the unfertile couples the chance to conceive, and also the advance of intracytoplasmic sperm injection (ICSI), a method involving the injection of one sperm into the protoplasm of a mature oocyte, has allowed the accomplishment of pregnancy for couples affected by severe male factor infertility, low fertilization rates after standard in vitro fertilization (IVF), or unexplained infertility. Though nowadays, a fertilization rate of roughly 70% is determined during ICSI cycles with a clinical pregnancy rate of 45%, total fertilization failure (TFF) still Happens in 1–3% of the overall ICSI cycles and might reoccur in following cycles, even once enough numbers of mature oocytes are available (Nasr-Esfahani et al., 2010). TFF is usually a physical misery and emotional stress for sterile couples. Therefore, it looks to be considerably vital to explore the reasons and get the solutions of TFF. There is currently a general agreement that oocyte activation deficiency should be principally responsible forTFF following standard ICSI (Vanden Meerschaut et al., 2014b).many studies have shown that the bulk of couples affected by TFF can have the benefit of assisted oocyte activation (AOA) following ICSI (Sfontouris et al., 2015; Bonte et al., 2019), and in some centers, AOA is sometimes offered to couples experiencing TFF or a really low fertilization rate after being well advised. The well-established and most typically represented AOA protocols will be classified into 3 totally different methods, i.e., mechanical, electrical, and chemical stimuli (Nasr-Esfahani et al., 2010).
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