ISSN 2348-313X (Print) International Journal of Life Sciences Research ISSN 2348-3148 (online) Vol. 10, Issue 4, pp: (73-81), Month: October - December 2022, Available at: www.researchpublish.com
Antioxidant Activity of Fresh Water Algae (Lyngbya kützingii and Microspora tumidula) From a Village in Kasur Aneeza Attique1, Uzma Hanif 1, Ghazala B. 2, Muzammal Abbas1, Muhammad Fahad Shakeel1, Ifra Aslam3, Misha Arshad4, Shumaila Rasheed1 Department of Botany Government College University Lahore, Pakistan 1, Institute of Botany, University of the Punjab, Pakistan2, Federal Medical College, Islamabad3, Pakistan, Sustainable Development Study Center, Government College University Lahore, Pakistan 4 DOI: https://doi.org/10.5281/zenodo.7466955
Published Date: 21-December-2022
Abstract: Free radicals interfere with the equilibrium of cells and tissues, which can lead to cancer. Fresh water algae such as Microspora tumidula and Lyngbya kützingii is a great source of secondary antioxidant metabolites. These metabolites most likely work well in the therapy of cancer. Algae exhibit huge variety of pigments not only chlorophyll, Carotenoids, phycobilins, and xanthophylls are the most prevalent of these. In the beginning, the medicinal effect of microalgae biomass was studied when it was used as pills, powder, and water additives. More and more studies in recent years have focused on finding and using useful medicinal components in algae. Aim of this study to evaluate the antioxidant role of algae in pharmaceutical industries. In the current investigation, the algal extracts were prepared by using three solvents methanol, chloroform and n-hexane to know about antioxidant potential of algae of specific area. To evaluate the antioxidant activity different test were performed such as DPPH, TAA, TPC, FRAP and MC. In Microspora tumidula 15.16% DPPH highest value was shown by methanolic extract. In FRAP Lyngbya kützingii showed maximum value in methanolic extract 64 µM Trolox mg -1. While the highest value of TPC by Lyngbya kützingii was shown in chloroform extracts 37.5µg GAE /mg. The results of total antioxidant activity (TAA) were showed that Lyngbya kützingii and Microspora tumidula both exhibited the highest value 154mg /g and 152mg/g respectively in methanolic extract. The result of metal chelating test showed highest value in chloroform extract 10.44% by Lyngbya kützingii. So both these algal species showed antioxidant potential. Keywords: Antioxidant potential, pharmaceutical industries, Evaluation, secondary metabolites, Cancer, Solvents extract.
I. INTRODUCTION Algae are photosynthetic and considered as special in nature because they show different phytometabolic contents and chemicals having various different structure and variety of biological functions [1]. Algae in addition to nutrient value also restrain various bioactive compounds like pigments (carotenoids and chlorophyll) amino-acids, phenolic compounds, mono and poly phenols [2]. These substances are vital in protecting algal cells from stressful and tension situations such as UV radiation, temperature changes, and nutrient rises and falls. In order to survive in these unfavorable conditions, they adapt by creating a large spectrum of secondary metabolites that are not seen in other species [3]. A term generally used as substance that protects cells from the damage caused by free radicals (unstable molecules made by the process of oxidation during normal metabolism) However, synthetic antioxidant may have a negative effect on consumer’s health and have become limited or restricted in some countries. Because of its putative and detrimental actions, such as carcinogenesis, the usage of synthetic antioxidants has declined [4]. More recent studies have been planned to explore the useful products from algal compounds not only to check the nature of these products but also investigate some specific applications for humans use in different fields of interest. Now an alga is also considered as rich source of for market benefits. These metabolites gain the special attention in the production of new products for pharmaceutical, cosmetic and food industries. Free radicals cause disease by causing oxidative damage [5].
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