DNA Transcription
Overview & RNA Polymerases
Transcription is the synthesis of RNA from a DNA template by RNA polymerase. Direction: always 5′→3′ on the new RNA strand, reading the template strand 3′→5′. No primer required. Three types in eukaryotes (Pol I, II, III), one in prokaryotes (+ σ factor).
5′→3′ synthesis
Template strand (antisense)
Coding strand (sense)
U replaces T in RNA
No primer needed
Ribonucleoside triphosphates
⚙ T H R E E E U KA R Y O T I C R N A P O LY M E R A S E S — P R O D U C T S, L O C AT I O N & Α - A M A N I T I N SENSITIVITY