International Research Journal of Engineering and Technology (IRJET)
e-ISSN: 2395-0056
Volume: 12 Issue: 12 | Dec 2025
p-ISSN: 2395-0072
www.irjet.net
PRELIMINARY PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY (STAPHYLOCOCCUS AUREUS AND PSEUDOMONAS AERUGINOSA) OF THESPESIA POPULNEA LEAF EXTRACTS BY DIFFERENT SOLVENTS Dr. K.L. Senthilkumar1, G. Deepan Chakravarthi2, S. Dhanalakshmi3, M. Dhanush Kumar4 1, Principal, Sri Vijay Vidyalaya College of Pharmacy, Dharmapuri, Tamil Nadu (India)
2, 3, 4, B. Pharm, Students, Sri Vijay Vidyalaya College of Pharmacy, Dharmapuri, Tamil Nadu (India)
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Abstract: The objective of the study is preliminary phytochemical screening and antimicrobial activity. The Thespesia populnea extract was made by using different solvents.
Method: The collected leaves were shade-dried and ground into a coarse powder. The powdered plant material was subjected to successive extraction using ethanol, chloroform, and ethyl acetate. The extracts were then analyzed for preliminary phytochemical constituents and tested for antimicrobial activity.
Conclusion: Thespesia populnea leaves have antibacterial activity against pathogenic microorganisms like Staphylococcus aureus and Pseudomonas aeruginosa. The given ethanol extract shows good antibacterial activity when compared to the chloroform and ethyl acetate. Key words: Thespesia populnea(L), Soxhlet method, phytochemical screening, anti-microbial activity.
I. INTRODUCTION Thespesia populnea (L.) Soland. Ex.Correa, commonly referred to as the ‘Portia tree’ or ‘Indian tulip tree,’ belongs to the family Malvaceae. It is a small- to medium-sized tree typically found along coastal regions. The tree attains a considerable height during its 15-metre growth [1]. The stem bark, leaves, flowers, and fruits have been traditionally used in the treatment of cutaneous infections, including scabies, psoriasis, eczema, ringworm, and guinea worm [2]. The plant was found to be rich in diverse phytochemicals such as alkaloids and glycosides, flavonoids, terpenoids, carbohydrates, proteins, steroids, and colouring agents. [3]. The present study was designed to determine the antimicrobial activity of ethanol, chloroform, and ethyl acetate leaf extract of Thespesia populnea to estimate MHA of the selected extract against Streptococcus aureus and Pseudomonas aeruginosa and to compare its antimicrobial activity with the well-known antibiotic chloramphenicol [4].
Fig; 01 Thespesia populnea leaf
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