International Research Journal of Engineering and Technology (IRJET)
e-ISSN: 2395-0056
Volume: 13 Issue: 01 | Jan 2026
p-ISSN: 2395-0072
www.irjet.net
Pharmacognostical and phytochemical analysis and identification of bioactive compounds using GC-MS Techniques of Ruellia tuberosa Dr. K. L. Senthil kumar1, Dr. P. D. Gokulan2 , Mr. S. Venkateshwaran3, G. Praveen Kumar4, S. Priyadharshini5, M. Punith Kumar6 1 Principal, Sri Vijay Vidyalaya College of Pharmacy, Dharmapuri, Tamilnadu, India
2 Head of the Department of Pharma ceutical Chemistry, Sri Vijay Vidyalaya College of Pharmacy, Dharmapuri, Tamil
nadu, India)
3* Associate professor, Sri Vijay Vidyalaya College of Pharmacy, Dharmapuri, Tamil Nadu (India)
*4, 5, 6 B. Pharm Students, Sri Vijay Vidyalaya College of Pharmacy, Dharmapuri, Tamil Nadu (India)
-----------------------------------------------------------------------***-------------------------------------------------------------------------------
Abstract- Aim: The aim of this study was to analyze the different bioactive compounds found in the leaves of Ruellia tuberosa collected from the Dharmapuri district. The study focused on identifying the bioactive constituents present in the chloroform extract and ethanol extracts of the Ruellia tuberosa leaves using the GC-MS technique along with phytochemical screening. Materials and methods: The collected leaves of Ruellia tuberosa were thoroughly washed, air-dried, and ground into a coarse powder. The leaf extract was prepared by using the Soxhlet extraction method. The selection of solvents is based on their polarity. Result: The phytochemical screening and GC-MS analysis confirmed the presence of various bioactive compounds in Ruellia tuberosa leaves. The study revealed several active chemical constituents, such as alkaloid, glycosides, flavonoids, proteins, tannins, terpenoids, etc. Conclusion: The present study shows that Ruellia tuberosa leaves contain a rich source of secondary metabolites. Key words: Ruellia tuberosa, extraction, pharma cogenetic studies, phytochemical analysis, and GC-MS technique.
Introduction Ruellia tuberosa is a medicinal plant that has been traditionally used since ancient times. It is an erect, perennial herb belonging to the family Acantharean. The plant is known by various names, such as Minnie root, fever root, snapdragon root, and sheep potato. It is commonly referred to as the “cracker plant.” The plant grows near the roadside in grasslands, cultivated fields, and even in a dry(xerophilic)area. It is a short-lived perennial plant with striking funnel-shaped violet bracteates flowers on dichotomous cymes. The fruit is a sub-cylindrical puberulent capsule having more or less 20 seeds per locule. The plant also has thick, fusiform tuberous roots that grow in clusters. Ruellia tuberosa L. is a tropical plant that is commonly found in many parts of Asia, including Indonesia, Malaysia, and India. The plant flowers and produces fruits from June to February. It can be easily recognized by its attractive, trumpet-shaped, violet-colored flowers with a five-lobed corolla. The plant is mainly grown in moist and shady environments. The plant attracts many pollinators, especially butterflies and other insects. The flowers appear light bluish-purple due to the presence of anthocyanin, a flavonoid pigment that is also used as a natural dye in industries. The plant contains several important phytochemical constituents, such as alkaloids, glycosides, flavonoids, tannins, proteins, terpenoids, diterpenes, sterols, etc. This study aims to investigate the extraction of bioactive compounds from Ruellia tuberosa using different solvents and evaluate the phytochemical content using Gas chromatographymass spectrometry analysis
© 2026, IRJET
|
Impact Factor value: 8.315
|
ISO 9001:2008 Certified Journal
|
Page 418