International Research Journal of Engineering and Technology (IRJET)
e-ISSN: 2395-0056
Volume: 10 Issue: 04 | Apr 2023
p-ISSN: 2395-0072
www.irjet.net
A Review on Extracellular Matrix Synthesis Using Collagen Dressings with Plant Extract Venkata Krishnan. S1, Bidyut Bikash Pathak2 and R. Nirmala3* 1PG Student, Department of Biotechnology, Hindustan College of Arts and Science, Affiliated to University of
Madras, Padur, Chennai – 603 103, India
2Research Scholar, Department of Biotechnology, Hindustan College of Arts and Science, Affiliated to University of
Madras, Padur, Chennai – 603 103, India
3Assistant Professor, Department of Biotechnology, Hindustan College of Arts and Science, Affiliated to University
of Madras, Padur, Chennai – 603 103, India ---------------------------------------------------------------------***--------------------------------------------------------------------Abstract - In order to improve wound healing procedures, the goal of this work was to create three-dimensional porous collagen
composites (Col) containing polyphenol-rich wormwood extract and to evaluate how they interacted with human skin cells. Scanning electron microscopy was used to examine the ultrastructure of the scaffolds, and biodegradability and the release of bioactive chemicals were examined in a physiological setting. Using two in vitro experimental models, the interactions of composites in direct and indirect contact with human skin cells were assessed. In comparison to Col scaffold, ColWE scaffold had more porosity, a higher degree of swelling, and greater durability against enzymatic degradation. More wormwood extract content in composite scaffolds allowed for more effective regulation of polyphenolic release. Human dermal fibroblast and keratinocyte cell adhesion and proliferation were promoted by the ColWE 0.5 variant. The composite scaffold also promoted the production of skin extracellular matrix components. Our findings showed that ColWE composites with enhanced physical, chemical, and biological characteristics might be utilised in cutting-edge applications for wound healing. Key words: Extracellular matrix, Scaffold, Collagen dressing, Plant extract
1. INTRODUCTION Skin wounds have a lengthy healing process that involves four overlapping stages: inflammation, granulation tissue development, reepithelialization, and extracellular matrix remodelling (ECM). The natural healing process might be hampered by external circumstances like bacterial infections or internal ones like the patient's old age or illness, which can result in chronic wounds [1]. To speed up the healing processes, a variety of passive (gauzes, films, hydrocolloids, hydrogels, and foams) and active (biocomponents loaded in polymeric matrix) dressings were evaluated on various skin wound types [2]. The primary functions of a wound dressing are to preserve moisture while removing excessive quantities of exudate from the injury site, to permit gaseous exchange, and to offer defence against bacterial infection. Moreover, a wound dressing must be simple to remove from the wound, biodegradable, and biocompatible [3,4]. The primary structural element of ECM and the most researched natural polymer for tissue engineering applications is collagen (Col) [5-7]. Because to their high biocompatibility, simple adhesion, capacity for swelling, and protection of the wound bed, collagenic dressings have been used to speed up the healing of skin wounds [8]. Col matrices further offered an ideal three-dimensional (3D) microenvironment for cellular adhesion and proliferation at the sites of lesions, encouraging the creation of granulation tissue, re-epithelialization, and new ECM synthesis as part of the wound healing process. Plant extracts and their physiologically active constituents promoted the healing of skin wounds [9]. Wormwood, or Artemisia absinthium L., has long been used as a febrifuge, antiseptic, antifungal, and antibacterial agent [10,11]. Wormwood polyphenolic extracts also shown antioxidant and free radical scavenging properties, which may improve the healing process of wounds [12-14]. Due to their pharmacological action induced by regulated release of active molecules and longer contact extent with skin lesions, plant extract-polymeric constructions are currently receiving a lot of interest in wound healing applications [1517]. Recently, sheets made of collagens that were loaded with plant-derived restorative chemicals or whole plant extracts were created for use in skin tissue engineering. As comparison to Col scaffolds [16-18], Col dressings with polyphenols from Hamamelis virginiana had a better ability to inhibit chronic wound enzymes including myeloperoxidase and collagenase. Rats with infected cutaneous wounds were treated with 3D sponges of Col filled with triphala herbal extract, leading to rapid wound closure and tissue regeneration [19,20]. Col-chitosan scaffolds added with Aloe Vera gel displayed better physicochemical and biological characteristics, whereas Col-matrices with Astragalus polysaccharides were produced as potential wound dressings with angiogenic capabilities. The capacity for recruiting, attachment, and proliferation of fibroblasts [21,22].
© 2023, IRJET
|
Impact Factor value: 8.226
|
ISO 9001:2008 Certified Journal
|
Page 646