Biotechnology is the exploitation of biochemical potential of the microorganisms for the medical, agricultural and industrial purposes. In processing, either the microorganisms are eliminated to prevent spoilage or they are encouraged to grow resulting in the production of fermented products. This study deals with the operating condition and comparison of enzyme production and purification of α-amylase from mutated and non- mutated Aspergillus tamarii. α- Amylase is an extracellular enzyme which can be produced by Aspergillus tamari using solid state and submerged fermentation.