The effect of dyeing and printing effluent on plant growth and biochemical constituents of Medicago sativa L. var. T9
was studied in a pot culture experiment. In the pot culture experiment, alfalfa plants were grown up to 60 days, in the soil
irrigated with different concentrations of dyeing and printing effluent (viz. 20%, 40%, 60%, 80% & 100%v/v). Each pot
containing sterilized soil. 20 Seeds were sown in each pot. All pots were irrigated (200ml) with respective concentration of test
solutions daily. Plants were thinned to a maximum of three per pots, after a week of germination. The higher dyeing and
printing effluent concentrations (above 40%) were found to affect plant growth and decreased chlorophyll-a, chlorophyll-b and
total chlorophyll, caroteinoids, free sugar, amino acids and protein contents, but diluted effluent (up to 40% ) favored the plant
growth and biochemical contents
