Label-based quantitation proteome analysis helps to understand the nature of these methods. iTRAQ utilizes isobaric
reagents to label the primary amines of peptides and proteins. 4-plex and 8-plex, which can be used to label all peptides from
different samples or treatments. It has high protein coverage and quantitative accuracy. Tandem Mass Tags (TMT) can be used
to identify and quantify proteins in different kinds of samples. Each TMT tagging reagent is composed of an amine-reactive
NHS-ester group, a spacer arm and an MS/MS reporter. Duplex, 6plex and 10plex are used to label different types of protein
samples. The efficiency and accuracy are high for TMT. Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)
technique performs protein labeling by adding light, medium, or heavy stable isotope-labeled essential amino acids (lysine and
arginine) to cell culture media. Efficiency of SILAC is higher. Its efficiency can be up to 100%. This review describes the above
methods and their advantages
