Air Pollution Tolerance Index is used to select plant species tolerant to Air pollution. Hyderabad is the capital of Indian State of Telengana and occupying 650 sqkms ,along the banks of Musi river. It is a metropolitan city with high population and traffic, dust, suspended particles, smoke and Heavy pollution in the Environment. We have collected Plant samples from our campus only. As the College is located in the heart of the city, there is high level of pollutants accumulated in the Environment. Screening of plants for their Sensitivity/Tolerance levels to air pollution, is important because the sensitive plants can serve as Bioindicators and tolerant plants as controlling the air pollution in Urban and Industrial areas. This Research work indicates the APTI and certain Biochemical properties of trees available in our campus. In this context 4 Physiological and Biochemical parameters have been studied like pH, Relative Water Content, Ascorbic Acid and Total Chlorophyll Content. l