Regeneration shoots from leaf explants of callus and In Vitro multiple shoots were obtained on MS Medium within
BAP, NAA, L-Glumatic acid and Kinetin , Coconut milk (CM). High frequency plant regeneration from leaf explants of S.
Nigrum by Ugander et al (2010) Callus induction and base explants of Aloe vera R. Prasad, Venkateshwarlu M et al (2018). MS
basal medium supplemented with various Auxons/Cytokinins BAP and NAA.Coconut water also had a rolein triggering the
formation of mult1ple shoots. Addition of BAP at 3.0 mg/l and NAA at 2.0 mg/l to the MS basal medium, induced regeneration
from the leaf segments. With an increase in the level of BAP 1.0 - 3.0 mg/l the percentage of explants producing shoots also
increased. The number of shoots developed on the leaf segments ranged from 1-4 to 2-3 by the addition o BAP at concentration
of 1.0 mg/I or NAA at 2.0 mg/l. Among he three concentrations of coconut milk used i.e, 5, 10 and 15% of coconut milk along
with 0.5 mg/l BAP proved to be ideal for multiple shoot
