ADSORPTION AND DESORPTION STUDIES USING ORGANICALLY MODIFIED CLINOPTILOLITE
Jog Raj, H. Farkaš, Z. Jakovčević, J. Bošnjak-Neumüller and M. Vasiljević PATENT CO, DOO., Serbia Reference: Poster 26, Page 127, WMF meets Asia, Jan 13-15, 2020, Bangkok, Thailand
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pH 3
Study MATERIALS & METHODS
The contamination of feed with mycotoxins is a big feed safety issue and it often leads to economic losses in animal production. Consequently, a variety of methods for the decontamination of feed has been developed, but the addition of mycotoxin detoxifiers (binders and modifiers) to the feed is the most commonly used method. Mycotoxin binders aim to prevent the absorption of mycotoxins in the intestinal tract of the animal by adsorbing the toxins to their surface.
Mycotoxin binders are generally clay (inorganic) or yeast-derived (organic) products, whereas mycotoxin modifiers are comprised of microbes or enzymes. Mycotoxin binders reduce the bioavailability of mycotoxins by binding and immobilizing them in the gastrointestinal tract of animals.
Adsorption/desorption assays consisted of suspending organically modified clinoptilolite (100 mg) in 10 mL of buffer solution (pH 3.0) spiked with AFB1, ZEN, OTA and FB1 (2 µg/mL of each mycotoxin).
The samples were incubated at 37°C for 30 minutes in an incubator shaker at 180 rpm. After centrifugation, the supernatant was analyzed by LC-MS/MS.
The remaining supernatant was removed, and the pellet was resuspended in pH 6.5 buffer, incubated at 37°C for 30 minutes, centrifuged, and the supernatant was analyzed by LC-MS/MS.
pH 6,5
AIM
RESULTS
Organically modified clinoptilolite had the greatest ability to bind AFB1 (100%), ZEN (92%), FB1 (88%), and OTA (89%) at pH 3.0 (Figure 1).
Figure 1: Percent adsorption for organically modified clinoptilolite (OMC) % Binding
AFB1
ZEN
FB1
OTA
Mycotoxins tested
In this study, in vitro tests were performed to determine the ability of organically modified clinoptilolite (MINAZEL PLUS) to adsorb: Aflatoxin B1 (AFB1) Zearalenone (ZEN) Ochratoxin A (OTA) Fumonisin B1 (FB1)
There was only minimal desorption of AFB1 (0.1%), ZEN (2.3%), FB1 (3.9%) and OTA (1.0%) from organically modified clinoptilolite at pH 6.5 (Figure 2). Figure 2: Percent desorption for organically modified clinoptilolite (OMC)
% Desorption
AFB1
ZEN
FB1
OTA
Tested Mycotoxins
CONCLUSIONS This adsorption and desorption data proves the efficacy of organically modified clinoptilolite against mycotoxins. These results were in correlation with the reports from George Rottinghaus, University of Missouri, USA.
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