Tổng hợp và ứng dụng nano bạc trên chất mang Alpha Cyclodextrin/Alginate sử dụng dịch chiết củ ngưu

N T T NGHI P

T NG H P B

NG D NG NANO T MANG

ALPHA CYCLODEXTRIN / ALGINATE S

D NG D CH CHI T C

1

2

L IM Trong th

is

nano tr

tv

M

U n c a khoa h

h t s c th i s

cs

u

c. p, y t , th c ph m, u khoa h c t v t li c kho Trong s

ov

Kh

u, ng d

c bi

t nano b

i

d

ng

i t d ch chi t c

[1].

c chung c a h u

kim lo c nano. c h i nh h

s d

t l a ch

ng

t ti

u

t qu

o lu n

ng xung i v i chi t xu t

ch

y tri n v

nv

ra

ng h p AgNPs. ,

b ng

t ng h p h t nano.

c nghi m

n s c kho con ng

t th c v t

B c

tb

d ng thi t b

kh c ph c nh ng v

u xanh

ng quan

t h p v i nhau t

quanh.

u ch h t nano b c c h i.

T n d ng ngu

cs

ion kim lo

c t ng h p b Arctium lappa Linn). C

l i ti u, h nhi t, h

d ng d ch b

ng huy

t u.

t ch c

c ti n c

b

th

p ch

ng g m: 4-Nitrophenol, Metyl Orange, Rhodamine B.

u bi

cv

h

ch

tr

n ng kh

c,

Vi c t ng h p ra h t nano kim lo i b

chi t c

c a composite AgNPs -CD/Alginate.

t tr i.

ch ng l

th

u ki n t ng h p composite AgNPs -CD/Alginate.

ng d ng h

ng ng d ng r

i

ng.

N

ng.

c bi t b i kh

n, ti t ki

nv

c ng d ng hi u qu trong cu c s ng,

ih

cs

hi u qu xanh, s c

n nh y

v

nh

uv im

thu t,

a

th p c

[2]. T nh ng h p v

ng d ng nano b

Cyclodextrin/Alginate s d ng d ch chi t c

n ch t mang Alpha

.

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3

4

t th p k

NG QUAN

c ng

i Nh t Norio Taniguchi

n s d ng thu t ng 1.1 Gi i thi u chung v 1.1.1 L ch s S

nano

tri n c a

ngh nano bao g m vi c x ho c m

nano

n c a khoa h

p nh

t

i Richard Zsigmondy,

Richard Feynman

t gi

c h p Hi p h i V

c

bi t v

h c

nano hi

is

nano hi

u th k 21

v v t ch t

i.

ib

n. t

t i Caltech

c

c a Feynman

c

nano hi

cho vi c

u t i Caltech. o lu t v

c ch

u

nano

nt Ch ng h

th

c ph

c m bi n sinh h c d ng

i thu

nh.

vi c s d ng v t li

th k 21.

ng d

trong

nh ng ng d ng v thi t b y t

m i

ng th ng G

nC

i. d n theo c p s nh

ng trong

u c a khoa h c qu c gia. V

t ng th ng Bill Clinton

m v kh . Gi thuy t c

c

nano. T i Hoa K ,

h

ng kho ng

c c

nc

ys

vi

di n thuy t v i t tr ng

n d ng v t li u b ng m t

.

m i n i tr i c a khoa h

N

cho r ng

ng c a c sinh ra t nh

xu t l

nano).

i

iv

t

ch

o

c ph

ph m,

c i ti n s n xu t

ih n

s d ng. ng l

c

ng.

c

Nanomedicine ( g m c i thi n vi t ,

1

kh

d

anotoxicology

i thu c, gi

iv i n s xu t

a v t li u nano)

c nano). M t s l

n n

i quan ng

ng. Nh ng m i

hi n

1

nano r t nhi

t li u ch

s c kh 1

i ti

t li u nano y t

l p ph

n c a thi t b y

, do thi u d li u n s c kho

iv

yv

c

n [3].

http://www.atomicarchive.com/Bios/FeynmanPhoto.shtml

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5

1.1.2

6

u trong qu

nano t ng v

hi

ng di n ra

k ,

c khoa h

c

1.1.3

ng d ng c a

tb

nano

o ra nh ng v t li

u l n b ng

ng th c ph

t khu

t l nh ho c m t s lo i h p th c ph m cao c p hi

n t , may m c, th c ph m,

b

Yh c

nhi u lo i

c ph m t l p nano

t vi khu n.

1.2 V t li u nano u tr

Nhi h n ch

c th nghi c

1.2.1 Kh

t

h t t

b ng tia laser h ng ngo i chi u t

i u. um td

ni m

V t li

i v t li

k

th

c nanomet, n l

c u li n k t hai l nh v c khoa h c nano v c ng ngh nano. K nano n m trong kho ng t v

c

bi t. Nh

ch t v t li u v i t

nv

i

ng nghi n

th

c v t li u

tr m nanomet, ph thu c v o b n

ch t c n nghi n c u [5].

con u tr

1.2.2 Ph n lo i

n nh ng b ph n c n thi t.

i v i tr nt

tr ng th

M t s s n ph m ph bi n

,v b c

c ph m t l p

l

ch y

ngh

u t o theo ki u cc

m tl

c nhi

ng

t nhi u l

t li u r n, r i m

V t li u nano kh ng chi u:

nano, i n t

May m c nano trong m t s lo i t nano b c

ct

n, u hi n nay

t li u

V t li u nano m t chi u:

d

l tr

i:

y l lo i v t li u c ba chi u

uc k

cho i n t . V d nh

th

c

m nano, h t

nano

h p.

c bi t.

nh

n ch t l ng v kh .

nano, kh ng c n chi u t do n

c c a pin s

p th

i ta t li

D

ng.

nanowhiskers.

a v t li ng th

khu n.

v

th c ph

Th c ph m

th ng [4].

c s d ng trong nhi yh

n

thu c s d ng trong thi t

, s n xu t, ng d ng v t li u, c

t nano b c

d nh

nano v

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hai chi u c k

c t do tr n m t chi u v hai chi u c

th

c

l i kh ng t do. V

ng nano, d

V t li u nano hai chi u:

t vi khu

y l lo i v t li u trong

i nt

y l lo i v t li u trong

m t chi u c k

c t do tr n hai chi u. V d nh m

th

c

m

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7

8

-

chia nh kh i v t li

th

cl th

ph D av

t

ch t v t li u th hi n s kh

i ta ph n th

c

bi t

k

th

-L

t

c

t li u t th

c nanomet nh c (nghi

,

h c sol - gel, l h

c nano

l p g

ph

lo i:

v

; kh

; kh h

V t li u nano kim lo i V t li u nano b n d n

-

V t li u nano t t

v i h t nano kim lo

i c,

V t li u nano sinh h c D

ih m nh

m:

i v i nhau ho c ph i h p hai

t

mm i

u qu . m: thi t b

i ta ph n lo i nh v t li

sau: H t nano kim lo i: trong

t

c ph n lo i theo h nh d

,

t ti n, t o ra ng nh

t

c

u

B ng 1. 1

c ph n

ch t [5].

1.4.1

m

ni m h t nano t nano. ct

n ISO

n 100 nanomet

th tl

Kh

m h t nano kim lo i d ng

t nano

ct

hi

i [8].

Ngu n g c , con ng

d

h t nano b c). N i ti ng nh t ph i k

t ng h p h t nano

Top - down

c nano t 1

t v t li u [6].

ng h p h t nano

p

ng h p h t nano

ngu n g c h t nano kim lo i

T h

xu ng

.

Kh ni m

nc

1.3.2

ng l n

1.4 H t nano kim lo i

1.3 H t nano

t m i so v i c

u ch

v t li u nano s t

V t li u nano t t nh sinh h c: trong

1.3.1 Kh

u, thi t

n.

Nh

c ph n lo i theo t nh ch t.

lo i theo t

tt

c nh b

cao.

i

m: ti n l

p

n ], [7].

n chi c c

ng th k th

c hi

. Chi c c c

Danh

c nx

trong c

Bottom - up

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t b

t nano kim lo i (h t

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c.

Danh

9

10

y trong chi c c c

c

c 70 nm.

um

th

uv

ng d ng c

T h ch t nhi t

t nano kim lo i m i th c s

thi t l

c c a h t nano gi m th nhi

y s gi m [5].

t cb

u. C

t

c a h t [8].

T nh ch t t Do s tr v

tr c

nt

c

Au, Ag,...)

i. Khi v t li u thu nh y v t li

kh i (

ch t

s

n n a,

im

tt

o i chuy n ti p Fe, Co, Ni)

s tt

tr

c nh s

n sang tr

n t . V t li u

thu n t

tr t t tr ng b ng t

]. 2: Chi c c c Lycurgus 1.4.2

2

b

1.5 H t nano b c

t h t nano kim lo i

1.5.1 S

T nh ch t quang

y l m t trong nh ng kim lo i n i ti ng v xu t hi n

t quang h c c a c

t hi

con ngu i t th i c

i.

Plasmon b m t

n ch t c a kim lo

b m

n ph m c a

t ngu n t hi

ng c

n t t do tr

ng c

nt t m

ng

ng c a

c c a h t nano kim lo i nh

x y ra. M

c s ng c cc a

nt

ng c ng h

ng plasmon b m t

h t nano ph thu c r t nhi

ng xung quanh c n

cu c s

B c

ti n ho c

n xu

plasmon

t nano

nt ib

c v kim lo i b c

ch M ts

,

y tinh. Ngo ra, b c c n

c s d ng trong ch t

a b c nh :

t kim lo i chuy n ti p

ng,

m m, d o, d

d n nhi

i

n cao nh t trong s

kim lo i. u: Ag nr tt tv

n tr c a kim lo i nh

nh

trong v ng d n [5].

Kh

: 107,87 g/mol

Kh

g/cm3.

Nhi 2

tv

ng. B

n

n t t do cao

b c,

u ch h p kim.

[5].

i

trang s m

t ph gia h p kim quan tr ng.

B c

c,

c d ng

y: 961,78 oC.

https://www.amusingplanet.com/2016/12/lycurgus-cup-piece-of-ancient-roman.html

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o

Nhi

12

C.

1.5.3

B

tinh khi t t i thi u 99,9 %

ng h p nano b c Nano b

n 99,999 % [9].

c t ng h p b ng nhi

m

u s d ng.

theo ng h p nano b c t

: 1.5.2

t

c.

ng d ng c a nano b c

Nano b

t lo i h t nano kim lo i, bao g

t 1-100 nm. Nano b

ng

t li h

t

c nano

i d ng dung d ch keo

b ic

i

ng ch n

Ph

g ph

v tl

tc

i polymer

th

Nano b

t nano b

c t o

t c a b c kh

n, d n nhi t, kh

-

c, d n

t quang h c. Nh hi

ng plasmon b m t, nano b

h p ph

t b chu

ti t c

d n

n, m c d

cu c s ng h ng

c a con ng

t,

g ph

,... C

tb

n th

t ti n [5], [14].

b ng

c ng d ng nhi u nh t trong t ng cs

th c t .

t o

v

d

kh ion b c t o kim lo i b c.

t t l i v i nhau cs d

Natri Borohydride

(NaBH4), Sodium Citrate, Acid Ascorbic, Hydrazine,... Vi c l a ch

gia d t nano b

kh

di t

h p tu thu

u ch

tl

ng h t nano

[5].

.B c

ng h n ch l n, bao g m

ng c a t i lo

t th

h p nano b c.

i trong y h c

i. Nano b c

mc

ho h c

p

nt ,c

c ng d ng r

khu n t t ch ng l i 650 lo i vi khu n, n m, th h y enzym v n chuy n ch

Ph

d ng nhi u nh

ng c y khoa, c m bi n sinh h c, s n xu

t

c

n ph

b c. c, tuy nhi

.

n cao [12], [13]. t khu n c a nano b

Kh

n,

n t (tia

b ph n

n nhi t t t, nano b n ph

kh ion b c t

v

ch t

c m bi n, thi t b quang h c, l c quang h

nt ,s

ng

m

t quang h c c a nano b

d

d

UV, tia laser, tia gamma) t li

c t ng h p b ng ph

c th c hi n b

quy n. M t s

n [10], [11].

B c c

ng

i ch t v d

ts d

n vi khu n b t ti

di t [11].

c h i, trong vi GVHD: TS. Nguy n Th

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kh n trong vi c lo i b nv

ng. Nh inh h

c n,

c gi m

ng c a

t l a ch n kh thi

u ch nano b c. S t ng h p qua trung gian sinh h c c GVHD: TS. Nguy n Th

t nano Danh

13

g

14

c gi i khoa h c ch cv

nv

h p nano b c v

ng.

c t ng

t cao b

khu n, n m, chi t xu t t

d

ng d ng r

c

c s

trong nhi

yt

t mang

1.6

gi i.

n t ng h p xanh AgNPs

ng, ch ng h n t o ra v t li

u ng d ng

th p k qua nh

uc

cs t

trong vi c s d

,c

c ng d ng

], [18], [19].

1.6.1 Gi i thi u v 4-Nitrophenol 4-Nitrophenol c

ng h p

c g i l para nitrophenol ho c 4-hydroxynitrobenzene,

t li

t nano t

Vi c k t h p c

polyme

cs t

polyme t carboxy t

t

i di n c a

ti

i polyme

v

v

y l m t h p ch

ng

c s d ng

ho t c a ch h

k tt

ng nh

t nano b c l i v i nhau. Ch t

(Chitosan).

nano b c c

i quy t v

t nano b c nh

h t nano b

c ng d ng r ng

n v ng

t nano b c theo th t

u trong

ng v v

c c ng th c ph n t C6H5NO3.

d

d ng d ch chi t c

a nano b c

t nano kim lo

C

c nh ng

c khoa h c [12].

nhanh c

ng

Arctium lappa Linn).

t li u kim lo

u qu c

m t ch ].

ch t mang

u c

nh c a Ag.

kim lo

ch t mang Alpha Cyclodextrin/Alginate b

mb o ra s

ng c a

u so v

d

u ch t o kim lo uh ts c

s

tr

ul

y

ng [14], [15]. ng h p nano kim lo

c ch t o t kim lo

c xanh,

p, nv

nhi u so v

t

t mang. B

t nano t chi t xu t th c v t

nhi u ti gi

Nh ng h t nano kim lo

th ng sinh h

th c v t. Trong s

ng h

.

n, hi u qu v kinh t

t t 3

i. D a t, gelatin, sodium alginate,

cs d

ch t o v t li u nano b c polyme

c [11], [16].

p ch

4thu c di t c , thu c tr c

c c u t o c a 4-Nitrophenol

c th

c s d ng r c nhu m, thu c di t n m v t, kh

Danh

hi n di n ov

ng Hoa k (United States Environmental Protection Agency, vi t t

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n xu t

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),

Danh

15

ch

16

m nguy hi m cho s c kho

trong m

kh

i. V

n h y sinh h

nh cao yn

hi

ch

i

hi m c a h p ch t h

c bi t h p ch

n [20]. t h p ch

n,

ch i

v

th n kinh

a con

ng v t. B

c ch chuy

ng

y, vi c kh ho c chuy p.

,

i 4-Nitrophenol tr h t nano kim lo

b

cs

l nh v c ph n t

kh

c s d ng c

1.6.3 Gi i thi u v Rhodamine B

t ch t b t tinh

C28H31ClN2O3)

c s d ng r

t trong nh ng

p d t may, gi y, th c ph m,

m ph m.

ng tinh th

d ng b

c h i, tan t t

c, ethanol, methanol [24].

c ngu i, d tan

c s d ng l m ch t ch th trong c

nhau.

5 4

c c u t o c a Rhodamine B

c c u t o c a Methyl Orange

Meth ch t h

nh

t v i trong nhi u ph n ng kh [23].

thu c nhu m

n

t tr ch i

t nano kim lo

Rhodamine B

h u c , kh

ng,

c bi t c

y thu c nhu m. Trong s

Methyl Orange (c c ng th c ph n t C14H14N3NaO3S)

m pc

thu c nhu m d

].

da cam, kh ng tan trong dung m

ms

ng v t th y sinh.

n

b i ti m

c n ng. Methyl Orange th

c nhu

th c v

1.6.2 Gi i thi u v Methyl Orange

th m

tv

p ch

m quan tr ng c

AgNPs i th p c

c ng

ng v t [20].

c kh kim lo

i s d ng [22].

c x thu c nhu m nguy hi m t

4-Nitrophenol

h ng ty th

iv

t ch ng h p

c s d ng r

nghi p. Khi ti ng h

t

may, in n, s n xu t gi y,... Trong th i gian g

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v

p

s ac

nh

c, palladium

h

], [25].

m h ng,

c th i n ng a da, m t, nh h

.M tl nn c l a ch

n

t nano kim lo thu c nhu m

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17

1.7

18

-CD

u trong t ng h p xanh nano kim lo i b

m

n

u t o gh c

mang -CD/Alginate

-

.

1.7.1 Alpha Cyclodextrin ( -CD)

t tinh b t. Cyclodextrins Villiers, ch

c ph

nh

c t ng h p t s ph n h y enzyme c a

hi n l n

u ti n v

c t ng h p t carbohydrate v i x

vi khu n Bacillus macerans. Sau x c c xem l cha

, nh

ho

n m 1891 b i Antoine

t

cb

h c v

nh ba cyclodextrin t nhi n l

tia X b i

n n m 1942, c u tr

c a -CD

v

m

p c .

y

c bao

u oxy l

c [29], [30], [27], [31].

ti t b i

vi khu n h c Franz

-CD, -CD v

-CD.

c a cyclodextrin n n trong nhi u n m

Schardinger dextrins

c hai

b c b i khung carbon v n

ng

ng c m t

1.7.1.1 Ngu n g c c a Alpha Cyclodextrin

c. Do s

ng

cg cx

nh b ng

[26], [27].

1.7.1.2 C u t o c a Alpha Cyclodextrin : (C6H10O5)6 -CD

Cyclomaltohexaose, Cyclohexapentylose) thu c h -D-glucopyranose n i v i

nhau b i

t -1,4-glycoside [28].

7

m c u t o c a -CD

cc a -

t

n 0,53 nm,

nm, chi u cao 0,79 nm [27], [31]. 1.7.1.3

t

ng d ng c a Alpha Cyclodextrin

T nh ch t Tr ng th S

6 C u t o Alpha Cyclodextrin GVHD: TS. Nguy n Th

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n v glucopyranose

D ng tinh th b t m

tr ng

6

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19

Kh

(g/mol)

972

th b th

c

14,5

ru t [30], [34].

nh

25 C 0,174

c ng d ng r

(nm3) ch y (oC)

Nhi B ng 1. 2

ph

278

tv

-CD,

-

c li a -

nh. S t

Hi n nay, s

10% c

cho r ng s d ng -CD

c

cc

(ch th -

n ph m c

ch a m t ph n t

[32], [33].

th

t s ngu

ph gia th c ph

y, -CD

n bao g ra,

n Hoa K th

ng

h tr

h

gi , b

-CD v

c

t ph n t

-

cc

1.7.2 Sodium Alginate (Natri Alginate)

-CD s

i th

gi i, x p sau cellulose v l

-CD th

E.C.C. Stanford v i

m

tr

.

, vi c thay th

b ng

(hydroxypropyl) ho c m

b c ba, amin b c b n) s d n

cc

kh

ng h

-CD b n v m t h

h nh p

c chi t Laminaria, Macrocystis, Ascophyllum,

Eclonia, Lessonia, Durvillea

Sargassum. Azotobacter

c t ng Pseudomonas.

ng Alginate

c.

Natri

c s d ng nhi u nh t [39], [40], [41].

1.7.2.2 C u t o c a Sodium Alginate

n h y -CD t o ra

: (C6H7O6Na)n

t m ch th ng do -CD b m

n t n glucose). T

th y

th

t d n xu t polysaccharide tuy

acid t ng. S th y ng acid y

CD

i Anh

.

xu t nhi u nh t t t

A

ch th

hi n b

bao g m Acid Alginic, mu i Canxi, Kali, Amoni c a Acid Alginic th

], [30], [35], [36].

ydrochloric, Acid S

u th

c ph

h p b i m t s vi khu

n k t qu c i thi

So v

cs d

1.7.2.1 Ngu n g c c a Sodium Alginate

v i -

-CD

c

[37], [38].

i polymer sinh h c bi

m nh gi

ng

u

ng u

n Nh t B n. a

ng m

a ph n t

n xu t c

li u th p b

t

-CD d

th ).

bao b

-CD

c ph m, m

-CD, 15% l

c h p v i nh ng

n ph m d

c a -CD [27], [31] p kh

host -

ng

ng d ng

khoang b n trong

m t ph c h p

ih

o

(g/100mL) Th t

20

i ki

nhi

, -

Alginic, g t v i nhau b

cao v c

-D-Mannuronic t 1,4-glycoside. Vi c chuy

Sodium Alginate nh m h tr kh

GVHD: TS. Nguy n Th

Danh

a Acid

-L-Guluronic acid (G) i Acid cc

GVHD: TS. Nguy n Th

Danh

22

21

t ng

i nhau theo ba lo i kh i: poly-G (G-G-G-

ng

G), poly-M (M-M-M-M) ho c poly-GM (GM-GM) [42], [43], [39].

c, m ch polymer h p th polymer.

o ra m t m ng ba chi bi n nh

tan trong

u ch hydrogel t dung d ch Alginate

th pv hai

t ngang ion, ch ng h

ion Ca2+

hai ch

Guluronate (kh i G) c a chu i Alginate ph i h p cao.

i G c a m t polymer t o m i n i v

1.7.2.3 T nh ch t v

t m nh nh ch

i c

i G c a chu i n

ion Ca2+ nh

tv iG

polymer li n k

8 C u t o c a Sodium Alginate

i m ch

nc

Tuy

c s d ng th p [42], [45].

ng d ng c a Sodium Alginate

t t polymer sinh h c

c s d ng r ng r i trong c ng nghi p

v n c s n trong t nhi n, c kh n ng t kh

ng th

sinh h c, ph n h y sinh h c,

c h i v chi ph th p. Sodium Alginate d h

d ch keo nh t c h

d

v

c, t o dung

nh t cao,

hloroform, Ether. Ngo

u

ra, Sodium Alginate d b c t m ch b i c

y u t acid, ki m m nh, nhi

cao, enzyme. Khi t

ng t

chuy n th

ng

ch t n

Acid Alginic, do

i ta l i d ng t

v i acid v c th tinh ch Acid

Alginic trong c ng nghi p [44], [42], [39]. e c

nh t.

9 S k t h p gi a ion Ca2+

c th n s ng

nh t t l thu n v i chi

ng h

nh t

(CaSO4, CaSO3).

ion Ca2+ g tr

n

ch

ng d ng

c a Alginate. Trong m t s th p v i s hi n di n c a

Trong y h c t c ph m r t quan tr

t v i Alginate t nh, l

nh t c a dung d ch [44].

n

c, t o gel, v t li u t

c

h i. M

m n i tr i c

cs d chuy n d

o gel.

i d ng hydrogel trong y sinh, bao g m ch c ch

c thu c) v

ng n

ct

GVHD: TS. Nguy n Th

Polymer Danh

Alginate ho , m t s s n ph

nv c t Alginate

cs d

gi

cd u tr tri u ch ng

GVHD: TS. Nguy n Th

Danh

23

c qu n. M t s chuy

24

u ti t l r

n th c qu

di

ch ti t c a d

i

gi m d ch ti t acid

ng trong vi

m ch

c a Lactose,

c u tr b

c cho kem,

c nh .

m thi u s bi c gi

ki

h

tr

tim, chi u d

b ov

ho c t m nh t h p th

n th

nh

t th cung c p n

n

uc

.

cM u

ph bi n

b ov A

b

2 cm, m c

i v i y h c c truy n Trung Qu c [2], [48], [49].

t th

qu

ng k

m qua. C , h c

Vi

c ph l ng t . Hoa v ng tr n c

c s d ng tr li u n

c c a gel.

c hi

kho ng 50 cm c n chi u r ng kho ng 30 cm, m

gel Alginate. N x

ng s ng hai n m, cao kho ng 1,5 m. L c y to r ng c

m trong khi m m u

gel A

cho v t th

c i m c a c a ng u b

lo i c y th n th o l n, th

M t lo

gi

gi

Gi

tan ch y [44], [47].

C y ng u b g (t n khoa h c l Arctium lappa Linn) thu c h Asteraceae, l

u ki n

a protein v

n h y cho u

ch

i thu

k t h p v i Alginate d

i

chung, t

v n chuy

t ch t tuy t v i cho vi c v n chuy n

lo i thu c protein, b i

c

m n cho kem, t o gel,

1.7.3 C ng u b 1.7.3.1

u

protein kh i s

nh

m [46].

Gel c th

n b t, t

B

ng m

ng Alginate h p th d ch ti t ra t v c cho v t th

o

ng

m thi u kh

]. Trong c ng nghi p th c ph m Sodium Alginate l

r

n v ng t

Alginate t th c ph

t lo i ph gia th c ph m t

th

ph m, c i thi n ch

nhi

. ng s n ph

.

cao ch ng h ngon mi

n

(A)3

c t

g (B)4

pt

, n c n ch a c

th

vitamin, protein, carbohydrate, kho 3

Danh

h c ng u b

cx

nh

trong th p k qua bao g m: lignans, terpenoids, polyphenols, polysaccharide

4

GVHD: TS. Nguy n Th

ph n h

n ch y u

B n c nh

c, c ch

1.7.3.2 Th

n

n xu t. t

s t

ng

ng ho c b oxy h

ph m th

b

p th c ph m.

nhi b

10

c ng d ng

ph ch t

ng: amino acid, kim lo i, [2], [48].

http://tapchitrungnien.com/cay-nguu-bang-cao-luong-my-vi-tri-bach-benh-cua-nguoi-nhat.html http://imgsg.blogspot.com/2016/10/cu-nguu-bang-burdock.html?m=0

GVHD: TS. Nguy n Th

Danh

25

1.7.3.3 C ng d C

26

ct

cl

nghi n c u

ch ra r ng c y ng u b

l i ti u, h nhi t, kh

sinh, t

b nh ti

virus [2], [48].

ng, kh

c c

ho t t nh sinh h c nh

iv is

chi t t l ,

p ch

m c

c bi t, c ng u b

c trong m , th

b nh tr n da.

ng c n t y. c ch s

i u tr . Ch ng h n m t s th

c th gi i

ng c a vi sinh v t

ph n h a h c c trong c

y tu n ho n m

y. H n th n a, c ng u b

c a ch t kh

C

c ng tr nh nghi n c u

c

t n

Foliatini b

nh.

i s chi u x

t l n th

nhau. Trong ph

ng ph

h t Alginate, sau l 2,45

kh tr th nh

v

c h t d

c a Alginate/mu i kim lo i nh h

h

th ng th i v i s

k t qu

ba ph

h t ion b c

ng ph

c 28,56

kh

th

ba

c 2,61

t nano b c

b

t N-

c hi u qu t t nh t [50].

s d ng b c nitrat

t

nh. Vi c kh

c th c hi n

GVHD: TS. Nguy n Th

34 nm. H

n ch ng l i vi khu

c nhu m h

v thu c nhu m azo)

ct

Danh

AgNO3

(ch y

n nitro-amines ng h p nano b c t l

t nano b

t nano b c t

u

c t ng h

AgNO3, t l ch t ph n ng, th i

ph n ng trong vi c t ng h p h t 25oC

n

n ph m ph c

ng. Sau khi t

gian ph n

nc u

ra b

c Cicer arietinum). ng gi m

r ng

mono-(1H-triazolylmethyl)-2-methylacrylt kh

c

Escherichia coli) [53].

h y ch

nhi nh trong th

ho t

ch

ng s

0,92 nm. T

vi c t ng h p nano b c n

t nano b c qua quang ph UV-Vis v i b

c

c b kh

u cho th y

c Azadirachta indica). D ch chi t c

(Staphylococcus aureus

o gel t o ra

13,45 nm. th

,

ng nghi p t ng h p nano b c b

c t ng h p v i

t Alginate trong

a Alginate t o ra h

ns

ch

c a Alginate/mu i kim lo i, p

m trong kho ng 436 - 446 nm v k ch th th n a,

c h p th b i c

t nano b c th pv

ng l n

k

u ch nh t l n

t kh .

kh b ng Natri Borohydride (NaBH4) t o ra h t c k

l p ph

t n

ion [52].

nghi n c u t ng h p h t nano

0,62 nm.

ch

t kh , v

c c a h t nano. T l

m 2015,

u ti n c

t nano

y kh

c hi u qu v c ng to l n

c u ng d a v

ng c

mu i AgNO3

s

1.8.1 T nh h nh nghi n c u tr n th gi i

b c-Alginate nh m m c

b c nitrat,

ng h p xanh

Alginate

t o v ch a

y

c ng s

in

nh ho c th i gian ph n ng [51].

d ng d ch chi t c N m 2013, Shihong Lin v c

d ng ch t kh

ng s

1-2

trong vi c lo i b kim lo i n ng ra kh i c th ch ng ta [2]. 1.8

nv uv

Alginate. T

p ch t ch ng b nh ti

d ng ch t

u ch nh t 30 - 100 nm d

n

c t m th y

l n b m t da, t

.

c h i ho c ch t kh b

i v i d ch

c s d ng nhi u nh t trong c

t ch yt i

th

nhau

n c a kh

trong khoang mi ng.

kh t

ng h th ng ti u h a, i u tr ung th , ch ng

b n trong h t, m t s h p ch t c ho ch hi u qu

nhi

c t ng h p t i n i t l d ch chi

i ta th y dung d ch 1 mM ct

GVHD: TS. Nguy n Th

t nano

Danh

27

b

28

c th hi n qua quang ph UV-Vis v i

88,8 nm v

cs

-13,6 mV. Ho

c th c hi n

t

Staphylococcus aureus

n c a h t nano b c

n

Escherichia coli tt

m ch ng l i vi khu n E. coli

P.

[57].

1.8.2

u trong n

Aeruginosa [54].

i h c Ramkhamhaeng t

nanotubes/cotton d ng chi t xu t t

t ng h p nano b c. S n ph hi u qu s

c ch

n c a hai lo i vi khu

S. epidermidis

ng s c

u cho th y h t nano b

t

ng d

c

t khu

ng c a

n 95% trong m t th i gian

n.

nh Th M

= 7, nhi

Ph XRD cho th

mL/30 mL. S n ph

a tinh th trong vi c t ng h p nano b c .

ng

c ng c a r

r ng Glass Ionomer Cement (GIC), h t nano b

GIC theo t l tr ng l

ph n ng 70oC, t l th

%. ng c a vi

H kh

uv

t t

t kh . Qua vi

th

2 c 9,25 - 26

Camellia sinensis). c nh

b o qu n v i th

u t 5 - 10 nm.

nb

ng l

ng b hi n n E.coli

t t trong th i gian ti

t ng

S.aureus r t

[60]. ng h p nano b c t d ch chi

-ray

on microscopy (TEM) cho th y s

(t

t nano b

d ng Sodium Alginate (Na-

t o

(t tr

uv i nh m

AgNO3

vi c t ng h p h t nano b c v i ti n ch ch chi

S n ph m t o t

t nano b c-

t

ch chi t/n t nano b

b c nitrat

Biological Macromolecules

acid ascorbic (Vc)

t ng h p

ng t ng h p t i pH

nm [59].

khu n Escherichia coli v Staphylococcus aureus [56].

.

u ki n t dung d ch AgNO3 l

ng h p

n c a nano b c nh m ch ng l i ho

c t ng h

d ch chi

Terminalia catappa).

Electron Microscope (SEM), Energy-dispersive X-ray analysis (EDS) v UV-Vis.

i v i h tinh th l

u t ng h p nano

b c t dung d ch AgNO3 b h t nano bao g m n

y ho

Nh

th ng l

b ng quang ph X-ray diffraction (XRD), Particle Size Analyzer (PSA), Scanning

nc

c nhi m

c5-

nanotubes v

P.

u v vi c t ng

Mangifera indica) nh m m

h i r ng trong nha khoa

c ng s

c nh m x

ng n [58].

h p nano b c t

vi c s d ng ch t tr

t li u l c Ag-Nano/carbon

c s d ng trong h th ng l c n

khu n. K t qu

c h t t 70 -

Aeruginosa [55].

32,4

c

t ng h u c a Ti

h

ng tr c ti

Sauropus androgynous) ng d ng trong vi c gi t nano t

xanh methylene

xanh metylen gi c a nano b c trong vi

ng vi khu n

GVHD: TS. Nguy n Th

Danh

c

sang m t tr i. K

c

ng c t 13 - 33,4 nm [61].

GVHD: TS. Nguy n Th

Danh

29

30

d NaBH4

t ng h p nano b c. H kh

h p nano b

ut

AgNO3, n

. Ph n

c th c hi n

b

n kh

ng

dung d ch NH3, n nhi

TH C NGHI M

c h t nano

ng trong kho ng t 10 - 20 nm [62]. uv

1.9

i mm ic a

t

Vi c ng d

t li

n

trong

2.1.1 T ng h p nano b c Kh

c c khoa h c v c ng ngh . V t li u nano mang l i nh ng ng d ng tuy t v i trong c

l nh v c nh x

t ,yt ,x l m

t ng h p nano b c thu n t y, ng

nay

v t li u kim lo i ph n t n tr n ch t mang v i v th n thi n v i m quy t v

ng.

k t t nhanh c

c

c nhi u c ng tr

ph

ng ph

Kh

ng c a nhi

ac

Kh

ng c a th i gian ph n

ng

ng h

i

t nano b c theo th i gian th p

t li

v i

n c u, th nh ng hi n nay v n ch a c

Kh tr

ng ph

v t l v h a h c truy n th ng: quy tr

c bi t

i

th p, gi m thi u trong v ngo kh v

n

ch

h a ch t

c cho th y trong th nh,

h i, ch v

c vi c s d ng c

c nhi

, l tr , c y s m, l sen, l t

ng h p v

u

UV-Vis

ng d ng nano b

Cyclodextrin/Alginate s d ng d ch chi t c

n ch t mang Alpha .

a

ch n l

u trong m

cx r

ch c a ch t c n

nh [63].

c

nh n

V nh ng l do tr n, ch ng t

t ngo i - kh

h p th p th m

c h i. C c c ng tr nh nghi n c u

d ch chi t t th c v t nh cam, l ,

p ch

ph UV-Vis

2.1.2

n gi n, chi ph

ph n c a d ch chi t th c v t c t

t i s d ng c

ng h p nano b c.

cho ph n ng kh

ki

-CD/Alginate.

c ng ngh

ng h p nano b c.

ng: 4-Nitrophenol, Methyl Orange, Rhodamine B.

chi t xu t th c v t c t h n i tr i h n so v i c ph

ph n

n

t nghi n c u t ng h p

hai lo i h mang v i nhau l

ng h p nano b c.

ng

cs

nano b c d a tr n vi c gh

ng c a n

c t nh an to n, ti t ki m n

h

t ng h p nano b c:

Kh

nghi n c u

u qu c

u ki n t

cx

iv

v t ch t.

c s h p th c b

cx

h p th

nt n k t qu c a

ng g .

nt

u ki n b

ph

cl i tr

it ns

p th [63].

GVHD: TS. Nguy n Th

Danh

GVHD: TS. Nguy n Th

Danh

31

ngo i UV

c

ki n Vis

uc p th

ng s d

ng h p th b c x

h p th A (m

ng: 200 -

ng: 396 -

quang h c UV-Vis Hi

32

ng s d

1

. M i lo

h p th

200 - 800 nm [63].

nt

nh lu t Lam bert

quang A) c a dung d ch t l thu n v i n

m tt ns

nh. Ph h ng ngo i

i dao [63].

Beer:

C c a dung d ch

theo bi u th c:

tb (FT-IR)

h ng ngo i, ph k h ng ngo i bi

i hi

Bruker, TENSOR 27 FTIR -1

-1

n 500 cm quang)

ngh

i Fourier

c s d ng ph bi n nh t. Ph FT-IR

A = .l.C h p th (m

400 cm-

s ng t 4000

i 0,5 cm ,

c, Vi n Khoa h

u nano b c

c)

t

- Vi

Vi t Nam.

s h p th p dung d ch Ph UV-Vis ngh

u nano b

- Vi

c, Vi n Khoa h

Vi t Nam.

h ng ngo i FT-IR6

2 ch

n th zeta

Th

a h t nano. Th zeta

UV-Vis JASCO V-630 spectrophotometer5

1

i gi

c x n m gi

s ng. Ph h ng ngo chuy 5

ng quay b u b

nano

s ng th

ab m th t t k thu

nh

m t c a h t nano trong dung d ch (ch t keo). Th

h ng ngo i FT-IR B c x h ng ngo

quan tr ng nh m hi

c m ng (l p h p ph ph n ch t l

ng n m trong kho ng +100 mV

chuy

i c a dung d ch.

m tc ah t c a th zeta

n -100 mV.

khi h p th b c x h ng ngo i. Trong 6

https://picclick.co.uk/Jasco-V-630-Uv-vis-Scanning-Spectrophotometer-202495145864.html

GVHD: TS. Nguy n Th

Danh

http://trends.medicalexpo.com/bruker-optik-gmbh/project-96471-414039.html

GVHD: TS. Nguy n Th

Danh

33

34

ch c h t nano c th

M u r n nano b

t c a h t nano. s

ch t

nh b ng nhi u d ng

i [64].

ng tia X

Horiba, EMAX ENERGY EX-400 t i P

- Vi n V

sinh d ch t

M u nano b

t nano Horiba SZ-100 t i

Vi n Khoa h c V t li u ng d ng.

ph EDX8

4

t TGA - DTA t bao g m m nhi

thu t tr

tv t

c c a m t ch nhi 3

a

.

zeta7

ch

t vi sai DTA (Differential Thermal Analysis) EDX

2.1.5 Ph c EDS.

thu

n vi c ghi l i ph ch

kh

ng tia X (Energy-dispersive X-ray spectroscopy) vi t t t nh ah cc av tr nd a v tr

c x , ch y

nt c

m

n t . Khi ch

ng cao chi

t r n th

n

nt cs

nt

(m ho

nh

nhi

ph n tr m c

t trong m u [65].

o

c ti

cs d

t t nhi

ng tin nh

k

nh nhi

, tinh khi

[66].

v t r n, t tr

c a m i ch ng tin v

ng th i.

gi a m u chu n (m u so s

ho c th

chuy n pha.

.

nh n ph

t v nhi

u) theo nhi

i kh i

t r n. Vi c ghi h

h cc

it l

ng TGA (Thermogravimetric Analysis

khi m

c gia nhi u kh

ng c a m u theo nhi

u ki n cho tr

ng c a m u b m

c. K thu

ho c th i gian cs d

ph n h y, oxy

a, bay

8 7

http://www.horiba.com/scientific/products/x-ray-fluorescence-analysis/micro-xrf-analyzer/details/ex-250350-450-574/

https://www.retsch-technology.com/products/laser-light-scattering/sz-100/function-features/

GVHD: TS. Nguy n Th

Danh

GVHD: TS. Nguy n Th

Danh

35

36

,...

n kh

ch

t trong m u, s

nh nhi

m [67].

M u r n nano b c

t Setaram, LabSys Evo

c o

10

o

nhi H

n 800 C v i t

- Vi n

c -Vi

gia nhi t

Khoa h

ngh Vi t Nam.

6 n vi

nt

n vi

ng (FESEM)

nt

ng FESEM (Field Emission Scanning

Electron Microscope).

n vi ho

s ng, nh

nt

c gi

Hi

n t truy n qua n t

Transmission Electron Microscopy) c

i cao (HRTEM)

truy

quan tr ng d ng

v tr n

c

ng b i th b

c

it t o

ngu

ng

n t nh m t o ra m t ch m elec

p

it

ng. K t qu

ng. M

c

y

b

y u

c khu

c a

m t

nt nh

u y

nh [69].

t lo i c a TEM [68]. M u nano b

M u dung d ch nano b

9

c gi

ng. quan

y

nh trong

n th

tinh th . M

it ng k thu

c 1 nm.

n vi TEM (Transmission Electron Microscopy)

. HRTEM s d ng c

ng.

m t ho

m

ns

u

b

i m t ngu

u trong sinh h

i cao HRTEM (High Resolution m

d ng electron

zig-zag. dung nh ng chi ti t nh

hi

ng b

t TGA-DTA9

5 2.1.7

i cao (HRTEM)10

n t truy

n vi JEOL JEM2100,

cp

c

i Vi

c, Vi n Khoa h

ngh Vi t Nam.

i Vi n Khoa h c V t li u.

10

http://vienhoahoc.ac.vn/Tin-tuc/Tiem-luc-trang-thiet-bi/27733/thiet-bi-phan-tich-nhiet-dta-dsctga

GVHD: TS. Nguy n Th

Danh

https://www.jeol.co.jp/en/products/detail/JEM-2100.html

GVHD: TS. Nguy n Th

Danh

37

38

2.2 Th c nghi m

2.2.1.3 D ng c

t, thi t b

2.2.1

Becher lo i 100 mL, 250 mL, 1000 mL

ng c

Erlen lo i 100 mL 2.2.1.1 H a ch t

ng ong lo i 50 mL

Sodium Alginate (C6H7O6Na)n: s n ph m t China

C t lo i 4 cm, 1cm

Calcium Acetate Hydrate, 99%: s n ph m t Netherlands

Pipet lo i 1 mL, 5 mL, 10 mL

Silver Nitrate (AgNO3): s n ph m t Sigma

i 15 mL

Alpha Cyclodextrin Hydrate, 98+%: s n ph m t Hungary

Chai bi lo i 3 mL, 10 mL, 20 mL

4-Nitrophenol, 99%: s n ph m t India

B h c u lo i 250 mL

Metyl Orange: s n ph m t India

ng kim lo i,

Rhodamine B, 98+%, pure: s n ph m t India

y l c, ph u, gi y b

th c ph

c

y tinh, l th y tinh.

Acetone c ng nghi p c c t hai l n C ng u b v D ch v th

kh (Burdock Root): S n ph m c a C ng ty TNHH S n xu t gm iT nH

ng

2.2.2 Ti

m

2.2.2.1 Chu n b

t ti

c

Pha dung d ch Sodium Alginate theo t l 0,7g/100mL H2O C n 0,7 gam Sodium Alginate h a tan v

2.2.1.2 Thi t b C

thi t b

M

khu y t gia nhi t (Heidolph, Magnetic stirrer)

c s d ng trong qu tr

t ng h p bao g m:

nhi

ph

trong v

n t (Electronic analysis balance)

v it

1200 v

/ph

c c t hai l n, khu y t

trong 60 ph , r i em i

si u m

30 ph .

Pha dung d ch Calcium Acetate Hydrate theo t l 0,65g/100mL H2O

B r a si u m (Ultrasonic Steri-Cleaner) M

m

0,65 gam Calcium Acetate Hydrate

ly t m (Biobase, Centrifuge Machine)

khu y t

nhi

100

c c t hai l n,

it

ay (Heidolph, LABOROTA 4000eco) M

b m ch n kh ng

M

xay

Pha dung d ch AgNO3 theo t l 0,625g/20mL H2O

T s y

C n 0,625 gam AgNO3 h a tan v

T l nh

c c t hai l n, khu y t

nhi

it

GVHD: TS. Nguy n Th

Danh

GVHD: TS. Nguy n Th

Danh

39

40

Pha dung d ch Alpha Cyclodextrin theo t l 0,16g/100mL H2O C n 0,16 gam Alpha Cyclodextrin h a tan v t

nhi

100

Thuy t minh quy tr

c c t hai l n, khu y

it

C n 10 gam c ng u b

: kh

c xay nh cho v

250 mL, h a tan ti p v i trong 2 gi , gia nhi t sau khi un

Chu n b d ch chi t c ng u b

c

c c t hai l n. Sau o

100 C, h th ng sinh h

c b o qu n trong t l nh t 4 - 10 C D ch chi t c ng u b

c th c hi n theo s

quy tr

c u th y tinh un h i l u

c l m l nh li n t c, h n h p

ngu i r i l c b ng gi y l c o

b

ti n h

c d ch chi t. D ch chi t

s d ng trong c

th nghi m sau.

sau: A

B

C ng u bĂ ng

Xay nh M u nguyĂŞn li u

c c t 100 mL 7 H th un h i l u

Vi

ch chi t c l

h

c (B)

t d ng ch

ng h p nano b l t

T l gi a Sodium Alginate : -CD = 8 : 1.

L c

T l gi a Sodium Alginate : Calcium Acetate Hydrate = 4 : 1. T l AgNO3 = 5% (Sodium Alginate + -CD). D ch chi t 2. 1

tc

GVHD: TS. Nguy n Th

Danh

GVHD: TS. Nguy n Th

Danh

42

41

ng h p nano a)

Thuy t minh quy tr

ng h p nano blank (nano tr

ch t mang

:

Ta h

-CD/Alginate

t t 26,9

mL dung d ch Calcium Acetate Hydrate v

Ti

sau:

v it

yt

khu

Ti trong h n h

Dung d ch

Dung d ch Calcium

Sodium Alginate

Acetate Hydrate

c ph i tr n,

H n h p gel

l

n th nh

Ly tâm, r a v i

qua

n

it

m.

lo i b n

c c t nh m lo i b

h

p ch

i (m i

c

h n h p 54,7 mL dung d ch -CD, ti u, siêu â

v

h nh

r a ti p ba l n v

Khu

nhi

yt

nhi

khu

m u

m.

c c t hai l n.

H nh p theo r a th m ba l n v

n th nh

lo i b

c c t nh m lo i b

l

p ch

i (m i Cu

c m u nano tr

+

ch t mang -CD/Alginate.

Gel Alginate Khu Dung d ch -CD

u, siêu â

m, ly tâm, r a

qua c.

Gel -CD/Alginate

2. 2

ng h p nano tr

ch

-CD/Alginate

GVHD: TS. Nguy n Th

Danh

GVHD: TS. Nguy n Th

Danh

43

b)

ng h p nano b

44

ch t ma

H nh

-CD/Alginate

n th nh a ti p ba l n v

Sodium

Calcium

Alginate

Acetate Hydrate

l

AgNO3

-CD

Khu

qua c c t.

qua

m.

c c t nh m lo i b

c

Th c hi n vi c t o h n h p Ag+/ -CD song song v i vi c t o gel Alginate.

u,

Cho 2 mL dung d ch AgNO3

ch -CD r i khu y t it

m.

Ag+/ -CD

khu m

yt khu

G

qua m. c c t.

t

th m 30 n th nh

Ti p theo r a ba l n v

nhi t

lo i b

c c t nh m lo i b

p ch

i

y

c theo

(m i l

Ag+/ -CD/Alginate

c gel Ag+/ -CD/Alginate. Cho d ch chi t c D ch chi t c

D a vào i u

t l

ng u bàng

ki n t i u Khu

u, siêu â

u ki n t

qua

r a ba l n v

m.

kh ion Ag+

i ch nano b c

lo i b

t p ch t (m i l

s d

l n n ra

Cu

cm s y t i 90oC

petri th y v

ch

cc t

c c t.

S y ng h p nano b

v im

c dung d ch nano b c. Ti

Ly tâm, r a v i

AgNPs/ -CD/Alginate

2. 3

nhi

nh

n h p Ag+/ -C it

Khu u, siêu â Ly tâm, r a v i

i (m i

n

ph i tr

Gel Alginate

p ch

gel Alginate.

siêu â Khu u, siêu â Ly tâm, r a v i

lo i b

p theo.

-CD/Alginate

Thuy t minh quy tr h: Ta ti p

100 mL dung d

n nh t t 26,9 mL dung d it

tr

yt

nhi t

khu n t trong h n h

c ph i

GVHD: TS. Nguy n Th

Danh

nh

GVHD: TS. Nguy n Th

Danh

45

A

46

2.2.2.3 Kh

B

u ki n t

t ng h p AgNPs/ -CD/Alginate

C

ut nano b c v

u ki n t ng h p t

a) Kh

n

o

t cho s n ph m.

gel Ag+ -

ng c a n

t ng h p nano b c

Ch

li

gel mang ion Ag+ (g/mL H2O) l n l

N 8 (A) Gel Alginate, (B) h gel Ag+/ -CD/Alginate, (C) Nano b c

t: 0,1 g/mL H2O; 0,5 g/mL

H2O; 1,0 g/mL H2O; 1,5 g/mL H2O. Th

ch chi t: 0,1 mL

Nhi

B

A

ti

n ng: 90oC.

ti

Th i gian ti T

n

khu

Ti n h

kh o s : th

b n chai bi

d ch chi t c

c b c gi y b c) ng v i t l n p khu y t

trong th i gian

90oC v i t

9 Dung d ch nano b H2O r i ti

t 0,1 mL dung d ch

t cho

gel mang ion Ag+ khu

. Sau khi ph n ng k m.

l nl

ch s chuy n t m i chai bi pha lo

i 5 mL

UV-Vis b n m u dung d ch nano v

kh o

c. b) Kh

ng c a nhi

ph n

ng h p nano

b c

Ch Nhi

li ti

ti n ng l

T kh

n

t: 30oC, 50oC, 70oC, 90oC, 120oC.

gel mang ion Ag+, ta ch

ct l n

t

1,5 g/mL H2O. 10 M u r

Th i gian ti

c sau khi s y

Th GVHD: TS. Nguy n Th

Danh

n

ch chi t: 0,1 mL. GVHD: TS. Nguy n Th

Danh

47

T

48

2.2.2.4 Kh

khu

Ti n h

ch chi t c

g/mL H2O.

ng v

t

v it

ph n

d ch

m

FT-IR

FT-IR

khu y

c h t, th zeta

EDX FESEM HRTEM

ng h p

B ng 2. 1 Kh

nano b c

li

ti

2.2.2.5 Kh

Th i gian ti , 10

n ng l ,

, 14

t: 0

,

, 16

, 18

,4

,6

.

Th

T

ph n ng, ta ch

a) 4-Nitrophenol

c t l nhi

Nano b

o

t

C.

kh o s :

n ng kh 4-

4.

Chu n b th nghi m: Pha dung d ch 4-

gel mang ion Ag+

c b c gi y b c) ng v i t l n t becher

c kh

nitrophenol v

ch chi t c

p khu y t v i t

Khi ph n mL dung d

cs

trong vi c kh thu c nhu m.

khu y: 1200

Ti n h

H2

t nano b

ch chi t: 0,1 mL.

T kh

mL

h al a nano b c

, 80

gel mang ion Ag+: 1,5 g/mL H2O.

N

TGA-DTA

m u

c.

ng c a th i gian ph n

Ch

c

c h t, th zeta

TGA-DTA UV-Vis

kh

c) Kh

M u nano b c

m i 5 mL H2O r i ti

dung d ch nano v

M u nano tr ng

. Sau khi ph n ng

ch s chuy n t

u nano

h al

t cho

p khu y t trong th ,t

u ki n t

gel mang ion Ag+ l 1,5

c b c gi y b c) ng v i t l n

k

a nano b c

Sau k

kh o s :

n

nh h

t i 120oC.

khu

c th i gian c n kh i 5 mL H2O r i ti

0 mL n

g/mL

0,1 UV-Vis

c c t hai l n.

Pha dung d ch NaBH4 0,05 M: n

n 0,0095 gam NaBH4

mL

c c t hai l n.

Ti n h

kh

-

kh o s : mL dung d ch 4-Nitrophenol 1 mM

c.

i 10 mL

c c t hai l n. L y 0,5 mL dung d ch NaBH4 0,05 M th m Nitrophenol

GVHD: TS. Nguy n Th

Danh

ng

mL dung d ch 4-

ch anh.

GVHD: TS. Nguy n Th

Danh

49

b

50

ch trong cuvet, l c nh

ti

h n ng. Ph n ng kh

i ph UV-Vis theo th n 800 nm

nhi

Pha dung d ch NaBH4 0,0125

200

Sau khi ph n ng k

ch t

n

n 0,0024 gam NaBH4

mL

c c t hai l n.

Ti n h

kh o s : mL dung d ch Rhodamine B 1 mM

b) Methyl Orange

c c t hai l n.

Nano b

c kh

Methyl Orange v

L y 0,5 mL dung d ch NaBH4 0,0125 M th m

n ng kh

Rhodamine B

4.

ng

m: n 0,0033 gam Methyl Orange

n 800 nm. Sau khi ph n ng k

c c t hai l n.

Pha dung d ch NaBH4 n

ti

h n ng. Ph n ng

i ph UV-Vis theo th

Pha dung d ch Methyl Orange 0 mL n

mL dung d ch

ch anh.

ch trong cuvet, l c nh Chu n b

i 40 mL

200

, ta th y dung d ch c

h ng

chuy n sang k n 0,0095 gam NaBH4

mL

c c t hai l n.

Ti n h

kh o s : mL dung d ch Methyl Orange 1 mM

i 10 mL

c c t hai l n. L y 0,5 mL dung d ch NaBH4 0,05 M th m Orange

ng

mL dung d ch Methyl

ch anh.

ch trong cuvet, l c nh

c

ti

h n ng. Ph n

c

i ph UV-Vis theo th 800 nm. Sau khi ph n ng k

n ch t

cam chuy n sang

c) Rhodamine B

Nano b

trong vi c kh

Rhodamine B v Chu n b

4.

m:

Pha dung d ch Rhodamine B 0 mL n

n ng kh

n 0,0048 gam Rhodamine B

c c t hai l n.

GVHD: TS. Nguy n Th

Danh

GVHD: TS. Nguy n Th

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51

52

T QU 3.1 K t qu kh

u ki n t

3.1.1 K t qu kh

nh h

K t qu

O LU N

UV-Vis:

t ng h p AgNPs/ -CD/Alginate h Ag+/ -CD/Alginate

ng c a n

ng h p nano b c

li u ti M u

m:

N

Th

(g/mL)

d ch chi t

Nhi

Th i gian

T

ph n ng

ph n ng

khu y

o

(mL)

( C)

(ph )

o

1

0,1

0,1

90 C

120

1200

2

0,5

0,1

90oC

120

1200

3

1,0

0,1

90oC

120

1200

0,1

o

120

1200

4

1,5

B ng 3. 1 S li u kh

90 C +

gel Ag / -CD/Alginate

K t qu kh o s :

2 Ph UV-Vis c a dung d ch nano b c theo n th bi u di

1 Dung d ch nano b c v

h p th c

i theo n ng

(B)

l n

GVHD: TS. Nguy n Th

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53

54

Nh

K t qu kh o s : 3.1: khi t l n

D 1,5 g/mL) th

c a dung d

c c a dung d ch

n (t 0,1 g/mL

n

m d n (chuy n t

m). D t

t qu

UV-Vis cho th y

d n khi t l n

kho

dung d ch t

nh h p th c

c s ng t 436 -

nh ng v i c

m t c a nano b c cho th y dung d ch t g/mL

0,5 g/mL

x

c ch

h p th c (

ng plasmon b

i c a dung d ch t i t l 1,5 g/mL max)

h p th 2,641

ng nano b c nhi u nh t

l t

i n m trong

nh h p th ngh

h p th

a.u.) ch ng t ph n ng t

i (Amax)

ch nano b c. T i t l 0,1

y xu t hi n

o nano b c.

l n nh t (t l

h p th c

y, t l 1,5 g/mL 3 Dung d ch nano b c v i t l nhi

ng h p.

3.1.2 K t qu kh

nh h

ng c a nhi

ph n

ng

K t qu

UV-Vis:

h p nano b c

li u ti M u

m:

N

Th

(g/mL)

1 2 3

1,5 1,5 1,5

Nhi

Th i gian

T

d ch chi t

ph n ng

ph n ng

khu y

(mL)

(oC)

(ph )

0,1

30oC

120

1200

0,1

o

50 C

120

1200

0,1

o

70 C

120

1200

o

4

1,5

0,1

90 C

120

1200

5

1,5

0,1

120oC

120

1200

B ng 3. 2 S li u kh

ph n ng

GVHD: TS. Nguy n Th

Danh

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56

3.1.3 K t qu kh

ng c a th i gian ph n

ng

h p nano b c

li u ti M u

m:

N (g/mL)

1 2 3 4 4 Ph UV-Vis c a dung d ch nano b c theo nhi t th bi u di

h p th c

i theo nhi

(B)

Nh n x : D

t qu

khi t l nhi

UV-Vis cho th 30oC

c a dung d ch t

n 120oC

n m trong kho ng t 440 - 446 nm,

max

= 446 nm, Amax = 0,069 a.u.) o

Amax = 0,177 a.u.) nhi u ch ng t c ch

t. T i 120 C ng nano b

l t

cho qu

s

c t o ra nhi u nh

i

max

= 443 nm,

1,5 1,5 1,5

Nhi

Th i gian

T

d ch chi t

ph n ng

ph n ng

khu y

o

(mL)

( C)

(ph )

0,1

120oC

0,1 0,1 0,1

0

1200

o

20

1200

o

40

1200

o

60

1200

120 C 120 C 120 C o

5

1,5

0,1

120 C

80

1200

6

1,5

0,1

120oC

100

1200

7

1,5

0,1

120oC

8 9 10

1,5 1,5 1,5

0,1 0,1 0,1 B ng 3. 3 S li u kh

nh h p th t i

t i 120oC ( r

n

ng plasmon b

ch nano b c. p nh

it

nh h p th c

nh ng v i c

m t c a nano b c cho th y dung d ch t 30oC (

h p th c

1,5

Th

K t qu

120

1200

o

140

1200

o

160

1200

o

180

1200

120 C 120 C 120 C

th i gian ph n ng

UV-Vis:

h p th l nhi

120oC

ng h p.

GVHD: TS. Nguy n Th

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58

3.2 K t qu kh 3.2.1 K t qu

a th

a nano b c

c h t nano, th zeta

5 Ph UV-Vis c a dung d ch nano b c theo th i gian th bi u di

h p th c

i theo th i gian (B)

a)

Nh n x : D

t qu

UV-Vis cho th

khi t l th i gian c a dung d ch t

n

nano b c t

ch.

t 430 - 435 nm,

nh ng v i c

ch ng t dung d ch t m

)

it

i n m trong kho ng

ng plasmon b m t c a nano b c h p th c

nh t

h p th c u (0,360 - 0,372)

ra

ng nhi max

n

ng v i n ng

nh h p th c

ch nano b c.

gi

t l th

h p th c

i

n ng x y i v kinh t

= 434 nm, Amax = 0,360 a.u.)

ch n l t 6 K t qu

kh

GVHD: TS. Nguy n Th

Danh

th

c c a m u nano tr

c (B)

GVHD: TS. Nguy n Th

Danh

59

60

3.2.2 K t qu

7 K t qu

zeta c a nano tr

h ng ngo i FT-IR

c (B)

Nh n x : D

t qu

th

3.6) cho th

c h t c a m u nano tr

ah

nh c a m u

c

136 nm, nh

t nano.

i m u nano tr

hi n di n c a kim lo i b nh n ch ng t b

140,5 nm.

t gi a hai m u

ng nhi

th

c tr i qua chuy

t tr c ti p

nt

Th zeta

n th zeta c a dung d ch keo nano b

th

nc

h ng k thu

ng s hi n di n c a m

c

C-OH ho c c ng c th l

ng gi n C-

K t qu ph IR c a Sodium Alginate cho th y s xu t hi n c a nh t i

h

1627,86 cm-1 th hi n cho nh m C=O v t n s 1415,70 cm-1 th hi

nh 3471,74 cm-1 v nh m C-H t i t n s 2923,98 cm-1. T n s

n

gi n C-C c a v ng th m.

t

C-

ng.

tr GVHD: TS. Nguy n Th

nh 1029,95 cm-1 th hi n dao

c a th

xu hu ng b n v ng cao. s ng (ELS) m

C-C t i t n s 1153,39 cm-1,

ng gi n c a li n k t C-O trong nh

-42,6 mV

-57,2 mV.

ng gi

O-C c a -CD.

nh c a h keo. D

n th zeta c a dung d ch keo nano tr

y

hi n c a

c. nv

y

K t qu ph IR c a Alpha Cyclodextrin m t s hi n di n c a nh m O-H t i t n s 3383,02 cm-1 v nh m C-H t i t n s 2927,84 cm-1. H n n a c n c s xu t

n. T ah

m ch ng t l

ng

c

uv th

h ng ngo i c a nano b c

Nh n x :

ng trong m t dung d cc

iv

8 K t qu

c c a h mang -

ch td

ng h c (DLS), c

b

u

AgNPs/ -CD/Alginate

n, th

nh

CD/Alginate. Vi

ng

c

Danh

-OH ho

-1

nh 1126,39 cm xu t hi n

O-H ng t i ng t

-O-C

c tr ng cho c u

saccharide c a n . GVHD: TS. Nguy n Th

Danh

61

62

K t qu ph IR c a cao d ch chi t c ng u b ho c c th l nh

N-H trong c

c

t i

c

nh 1431,13 cm-1. Ngo

t C-

nh 3375,31 cm-1, nh m C-H t i

acid amin t i

nh 2931,70 cm-1 c ng v i nh m C=O t i

c ng th hi n nh m O-H

nh 1631,72 cm-1,

3.2.3 K t qu

EDX

-C

ra c ng c s xu t hi n

-OH ho

C-O-C t i t n

-1

s 1033,81 cm . S xu t hi n c a c

nh

n

l do s hi n di n c a c

th

ph n lignans, terpenoids, polyphenols, polysaccharide c trong d ch chi t c a ng u b

.

t

nh 3437,03 cm-1 v

K t qu ph n t

IR c a nano blank cho th y s di n di n c a nh -H t i t n s 2923,98 cm-1. T i

1431,13 cm-1 v 1091,67 cm-1 l th m, C-

t th hi n cho c

-OH ho c

nh

O-H

nh 1647,15 cm-1,

C=O, C-C c a v ng

ng C-O-C

ng c a

Sodium Alginate v Alpha Cyclodextrin.

-1

cm l

ng t i

cm-1,

acid amin t d ch chi t.

-H (t

-CD v Sodium Alginate). B n c nh

ng t

nh 1029,95 cm-1 l dao

ng C-C c

t C-

t n s 2923,98

t i

90

p ch

c h p th

80

C

800

70

-O-C t i

ng c a vi c sau khi

Ca

600

c

p ch t

56,7

60 50

37,8

40 30 20 10

400

c x h ng ngo i,

ng kim lo i b c cao nh t

100

,

nh 1423,42

-OH ho c

K thu t ph n t

u x tia X ch

ng

N-H trong c

nh 1654,86 cm-1,

ng c

9V

nh 3448,60 cm-1 c th l

Percent (w/w)

c a nh m O-H ho c nh

t i

Counts

K t qu ph IR c a nano b c

3,5

1,9

0 C

ng v i nhi u v n t c

O

Ca

Ag

xu t hi n d i ph h p th g

200

h p th b c x h ng ngo i.

O Ag

Ca

Ca

0 0

1

2

3

4

5

6

7

8

Energy (keV)

10 K t qu ph EDX c a nano b c

GVHD: TS. Nguy n Th

Danh

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Danh

63

64

3.2.4 K t qu

t TGA-DTA

120

Nh n x :

Alginate -CD Blank -CD/Alg AgNPs/ -CD/Alg

A 100

t qu

l

chi u x

m tm

ng kim lo i b c chi m nhi u nh

ng/Kh i l D

i

3,11 % (% Kh i

ng). k t qu ph EDX (H u c a kim lo i Ag.

60

40

u xu t hi n m nh t i

20

n

0

uc

g Sodium Alginate, -CD, d ch chi t c

80

Loss Weight (%)

D quang ph

hi n di n c

B

20

t Ca cho th y s

t ngang c a Alginate. M u nano b

a

Alginate 0

n tr m kh i l

HeatFlow

l n

-20

C (37,8 %), O (56,7 %), Ca (3,5 %), Ag (1,9 %).

-CD

-40 -60

Blank

-CD/Alg

-80

AgNPs/

-100

-CD/Alg

-120 100

200

300

11 K t qu ph

400

t tr

500

600

700

800

ng T

t vi

sai DTA (B) Nh n x : M u

Peak n 1 Giai

Sodium Alginate

o

Td ( C)/mloss

n3

n4

31-200/10

200-500/46

500-800/14

-

-

357,51

619,43

-

33-120/7

120-360/68

360-800/21

-

-

328,01

-

-

(%) Texo (oC)

-CD

n2

o

Td ( C)/mloss (%) Texo (oC)

GVHD: TS. Nguy n Th

Danh

GVHD: TS. Nguy n Th

Danh

65

Nano blank

Td (oC)/mloss

66

32-180/17

180-500/40

500-800/18

-

-

-

609,51 v

-

Alpha Cyclodextrin (M u b

(%)

n)

ng bi u di n TGA, khi nhi

Texo (oC)

ub m

635,10 AgNPs

nh y

Td (oC)/mloss

ub m

35-120/7

120-400/27

400-600/13

600-800/4

-

-

535,03

-

h

(%) Texo (oC) B ng 3. 4

t s li u k t qu

7 % kh

ng. Khi nhi

68 % kh

ng v i nhi

t ng t 33 t ng t 120

ng, l 328,01 C. Khi nhi

(giai

n 3), m u b m

21 % kh

ng. V y t ng kh

ng

ng

n h y.

ab

nh y

mloss l kh

ng m

ub m

Td (oC)/mloss (%): m

i 17 % kh

ub m

2)

t TGA. t

DTA.

o

C

v Nh m m c ng th

n

n 1)

ab

ub m ub m

b n

Sodium Alginate (M u b

nh y

3) th m u b m

n)

nhi ng bi u di n TGA, khi nhi ub m

ng. Khi nhi

ub m

t ng t

ng, l

n 3), m u b m

ng ng m

% kh

o

n 500 C

n 1) n

kh 49 % kh

ng. Khi nhi

13 % kh

ng

t ng t 600 V y t ng kh

ab

i 619,43 ng m

75 % kh

ng

t ng t 35

n 120 oC (giai

n 1)

n 400 oC

n 2)

n)

t ng t 120

o

t ng t 400

ng, t

535,03 oC. Khi nhi ng m u m

n 800 C (giai

n 600 C (giai

xu t hi n 800 oC (giai ng m

n

oxy h a n 4), ph n tram

51 % kh

ng

n h y.

ng DTA x

357,51 oC. Khi nhi

t

n 200 oC (giai

t ng t

ng. Khi nhi

27 % kh

t ng t 5

n o

n h y.

nh y

7 % kh

n 500 oC

u t hi

ng DTA. V y t ng kh ng

AgNPs (M u b

gia nhi t 10 oC/ph t.

t

ng

ng bi u di n TGA, khi nhi

C. V y t ng kh

n 180 oC (giai

t ng t 18

ng. Khi nhi

8 % kh

C

25 % kh

t nhi t c a h

ph n c

o

t ng t 32

ng. Khi nhi

0 % kh

n 3) th m u b m t a nhi t (oC), m

Texo l nhi

o

96 %

n)

C) ng bi u di n TGA, khi nhi

ng v i nhi

ng m

t Nano blank (M u b

h

n 800 oC

o

d

2)

n 2)

t ng t 360

kh

4 % kh

n 1)

n 360 oC

ng DTA x o

b

n 120 oC (giai

t ng t 5

n 800 oC (giai

t hi ng

i 619,43 % kh

ng

ab

n h y.

GVHD: TS. Nguy n Th

Danh

GVHD: TS. Nguy n Th

Danh

67

3.2.5 K t qu

68

o TEM

12 K t qu

TEM c a nano b c

i 20 nm

14 M t s

nh HRTEM c a nano b c

Nh n x : D h 13 Kh M ts

ng

s

th

ch t

i 20 nm

nh HRTEM:

t qu

CD/Alginate. C

Danh

ch chi t c t nano

hc u

keo t trong dung d ch. S n 10 nm v

GVHD: TS. Nguy n Th

3.13 cho th y h t nano b

ng v

th

ch

k

th

c t ng mang -

c h t nh

u,

c c a AgNPs ch y u t 3nm ng 5 nm.

GVHD: TS. Nguy n Th

Danh

69

70

3.2.6 K t qu

3.3 K t qu kh

kh

c a AgNPs

3.3.1 K t qu kh

y 4-Nitrophenol

K t qu kh o s

15 K t qu

ch nano b c 16 S

Nh D ngh

m v t li u r i so v

nh, h

g nh t trog dd

K t qu

do ch t mang ho c nano kim lo

c c a ph n ng ph

UV-Vis:

2,0

5

A

i ta s

kim lo i b

3

m tm

x ng n

t o ra s c ng d ng trong vi c d n thu c ho

2 1

1,0

Ln(At/A0)

c, nh

Absorbance (a.u.)

t dung d

B

4

ng ho 1,5

nh

y 4-NP

20 min

0 -1 -2 -3 -1 k= 2.39 x 10 sec 2 R = 0,874

-3

0,5

-4

h y sinh h c.

-5 0,0 200

-6 300

400

500

200

600

Wavelength (nm)

Danh

600

800

1000

Time (sec)

17 K t qu ph UV-Vis trong ph n ng ph

GVHD: TS. Nguy n Th

400

y 4-NP theo th i gian khi

GVHD: TS. Nguy n Th

Danh

1200

71

72

Nh hi

u ti

u su

i

il

t ho

u su t gi m xu a nano b

nh trong 5 l n th c ng 70 % ch ng

t.

3.3.2 K t qu kh

y Methyl Orange

K t qu kh o s

18 Hi u su t

c a 4-NP trong 6 l

p v i th i gian ph n ng

20 Nh n x : D

h 3.16 ta th y v c

NaBH4 v

nt

a dung d ch chuy n t

ng s i b t kh (do k

2

c sinh ra t ph n ng gi a

c).

D

3.17 cho th y c

19 S

h p th t i b

4-

ng ki m.

h

-

h p th t

x y ra d ng th i xu t hi n s t ng d

hi n di n c a n s gi m d

nh h p th m i t i b

nh h p th c a s n ph m) theo th i gian. ch

nt

-

m t nano b qu

t nh

4

v

AgNPs

nh c s ng

4

n t . Sau khi ion BH4

-NP h p th

t nano b c chuy n electron t ion BH4 a 4-NP b kh

c c a ph n ng gi a MO

Cu

-NP, k t -

kh

-AP (4-

GVHD: TS. Nguy n Th

Danh

GVHD: TS. Nguy n Th

Danh

73

K t qu

74

UV-Vis:

Nh n x :

2,5

9 ta th y

D

2

B

A

v c

1

nt

a dung d ch chuy n t

cam sang

ng s i b t kh .

2,0

1,5

Ln(At/A0)

Absorbance (a.u.)

0

1,0

D th

-2

gi m nhanh theo th

-3

10 min

h

300

400

500

600

h p th c

it ib

100

700

Wavelength (nm)

200

300

400

500

600

Time (sec)

20 K t qu ph UV-Vis trong ph n ng kh MO b i NaBH4 v i s

700

th

m t nano b

k t qu

c s ng 462 nm

c s ng

MO b i NaBH4 khi c p ch t m

-6

200

h p th t i b

ng th i xu t hi n s t ng d n

N=N-) c

-5 0,0

y MO x y ra ngay l p t c khi

-CD/Alginate

h p th c a s n ph m).

-4

0,5

3.20 ta th y ph n ng ph

-1

2.

nh do nh

(-

t ch

Sau khi ion BH4

MO h p

t nano b c chuy n electron t ion BH4

cam c a MO b kh

kh

t

c a AgNPs theo th i gian

T

21 cho th y hi u su t ph n ng

r t t t sau 6 l n th c hi n,

a nano b c v n t t sau nhi u l

21 Hi u su t

c a MO trong 6 l

p v i th i gian ph n ng 10

GVHD: TS. Nguy n Th

Danh

GVHD: TS. Nguy n Th

Danh

75

76

3.3.3 K t qu kh

y Rhodamine B

K t qu kh o s

22 S

c c a ph n ng gi a RhB

4

24 Hi u su

v

a RhB trong 6 l

p v i th i gian ph n ng

AgNPs K t qu

D

UV-Vis: s

3

22 ta th y s

y RhB

c.

c quan d

m d n theo th i gian t

h

8 6

D

2

Ln(At/A0)

Absorbance (a.u.)

4 2

14 min

CD/Alginate. Sau khi ion BH4 b cl

-6 -8

300

400

500

600

700

300

400

Wavelength (nm)

500

600

700

800

900

th

p th

-

m t nano b

n electron t ion BH4

RhB b kh

-10

0

h p th c a RhB t i b hi n di n c a c a h

-2 -4

1

3.23 cho th y c

gi m d n theo th i gian s

0

d

. Ph n

n

t nano h ng c a

y RhB di

kh

Time (sec)

23 K t qu ph UV-Vis trong ph n ng kh MO b i NaBH4 v i s

t

c a AgNPs theo th i gian

GVHD: TS. Nguy n Th

Danh

Hi u su t t gi m xu ng kho ng 80 %

24 cho th y i

l

u su t n l n th c hi n th 6.

GVHD: TS. Nguy n Th

Danh

77

78

K T LU N

KI N NGH

, h composite AgNPs -CD/Alginate h p th

c t ng

c xanh s d ng d ch chi t c

th n thi n v

ng. D ch chi t c

trong vi c kh ion Ag+

Kh

hi

t t

u th

t ng h p nano b c:

u ti nh

ng h p nano b c t. ng c

ng nh

i v i vi

-NP, MO, Rhb.

S d ng d ch chi t c

ng gel ch a ion Ag v

v

ng y u t

ch +

T l gi

th

u ki n t ng h p t

c. T nh ng k t qu

t s k t lu n nh u ki n t

ts h

ng d ch chi

ng Kh

0,1 mL d ch chi t).

cc ah .

o

Nhi

ph n ng: 120 C.

Th i gian ph n ng: 120 ph . T

khu u ki n t ng h p t

K t qu

t nano b

th

th

c h t cho th

136 nm k

: h

t qu

k h t

th

u

ch

k

i.

Th zeta c a m u nano b

-57,2 mV cho th

nh cao

trong dung d ch. K t qu EDX cho th K t qu % kh Ho t t

ng x

m

49 % kh

ng

ab

ng m

a nano b

51

n h y.

t : ng d ng t t trong vi

-Nitrophenol, Methyl Orange, Rhodamine B. M u nano b c

c ki m tra ho NaBH4

ng nano b y t ng kh i l

t nano b c t ng h ch

l

ng ph n ng kh 4-NP, MO, RhB v i

ng

trong th i gian l n

GVHD: TS. Nguy n Th

Danh

GVHD: TS. Nguy n Th

Danh

80

79

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PH L C

Ph l c 2 S Ph l c 1 S

th

th

c h t trong dung d ch c a m u nano b c

c h t trong dung d ch c a m u nano tr ng

GVHD: TS. Nguy n Th

Danh

GVHD: TS. Nguy n Th

Danh

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Ph l c 3 Th zeta c a dung d ch nano tr ng

Ph l c 4 Th zeta c a dung d ch nano b c

GVHD: TS. Nguy n Th

Danh

GVHD: TS. Nguy n Th

Danh

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92

Ph l c 6 K t qu ph FT-IR c a d ch chi t c

Ph l c 5 K t qu ph FT-IR c a -CD

GVHD: TS. Nguy n Th

Danh

GVHD: TS. Nguy n Th

Danh

93

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Ph l c 7 K t qu ph FT-IR c a nano blank

Ph l c 8 K t qu ph FT-IR c a AgNPs

GVHD: TS. Nguy n Th

Danh

GVHD: TS. Nguy n Th

Danh

95

96

Ph l c 9 K t qu ph FT-IR c a Sodium Alginate

Ph l c 10 K t qu ph EDX m u r n AgNPs

GVHD: TS. Nguy n Th

Danh

GVHD: TS. Nguy n Th

Danh

97

98

Ph l c 11 K t qu TGA-DTA c a -CD

Ph l c 12 K t qu TGA-DTA c a nano blank

GVHD: TS. Nguy n Th

Danh

GVHD: TS. Nguy n Th

Danh

99

100

Ph l c 14 M t s

nh TEM c a dung d ch AgNPs

Ph l c 13 K t qu TGA-DTA c a AgNPs

GVHD: TS. Nguy n Th

Danh

GVHD: TS. Nguy n Th

Danh

101

102

Ph l c 16 M t s

Ph l c 15 M t s

nh FESEM c a dung d ch AgNPs

nh HRTEM c a dung d ch AgNPs

GVHD: TS. Nguy n Th

Danh

GVHD: TS. Nguy n Th

Danh