Issuu

3349

CHARACTERIZATION OF DEEP WATER REEF COMMUNITIES WITHIN THE MARINE CONSERVATION DISTRICT, ST. THOMAS, U.S. VIRGIN ISLANDS

FINAL REPORT

Submitted to the Caribbean Fisheries Management Council April 30th, 2008 Prepared by Richard S. Nemeth, Tyler B. Smith, Jeremiah Blondeau, Elizabeth Kadison, Jacqulyn M. Calnan, and Jordan Gass Center for Marine and Environmental Studies University of the Virgin Islands

http://marsci.uvi.edu/

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

TABLE OF CONTENTS Table of Contents......................................................................................................................................... I List of Tables .............................................................................................................................................. III List of Figures ............................................................................................................................................IV List of Appendices ....................................................................................................................................VII Executive Summary .................................................................................................................................... 1 Introduction ................................................................................................................................................. 3 Background ............................................................................................................................................... 3 Methods........................................................................................................................................................ 8 Habitat Stratification and Sampling Design ............................................................................................... 8 In Situ Sampling Protocols ........................................................................................................................ 9 Benthic Composition .............................................................................................................................. 9 Coral Health ......................................................................................................................................... 10 Fish and Other Commercially Important Motile Resources ................................................................. 11 Results and recommendations................................................................................................................ 12 Benthic Composition................................................................................................................................ 12 Scleractinian Corals and Hydrocorals.................................................................................................. 15 Gorgonians and Sponges .................................................................................................................... 18 Algae .................................................................................................................................................... 18 Non-living Benthic Cover ..................................................................................................................... 21 Benthic Composition by Sampling Strata ................................................................................................ 23 Benthic Epifauna.................................................................................................................................. 23 Algae .................................................................................................................................................... 24 Non-living Benthic Composition........................................................................................................... 24 Predicted Versus Sampled Benthic Strata........................................................................................... 27 Algae ................................................................................................................................................ 27 Coral ................................................................................................................................................. 27 Pavement ......................................................................................................................................... 28 Sand ................................................................................................................................................. 28 Coral Health............................................................................................................................................. 31 Disease and Bleaching ........................................................................................................................ 31 Partial Mortality .................................................................................................................................... 33 Unknown Necrosis ............................................................................................................................... 34 Assemblage Structure of Fish and Motile Mega-Invertebrates ............................................................... 39 Diversity, Abundance and Biomass of Reef Fishes............................................................................. 39 Trophic composition among habitat strata........................................................................................... 49 I

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District Size structure of Reef Fishes............................................................................................................... 51 Conclusions and Recommendations...................................................................................................... 59 Improved Evaluation of EFH within the MCD.......................................................................................... 59 Expansion of the Evaluation of EFH Outside the MCD........................................................................... 60 ACKNOWLEDGEMENTS........................................................................................................................... 61 LITERATURE CITED.................................................................................................................................. 62 APPENDICES............................................................................................................................................. 65

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

LIST OF TABLES Table 1. Species known to spawn or form aggregations around the red hind spawning aggregation site (18.202 N, 65.002 W)............................................................................................................................. 5 Table 2. The ranked percent coverage and percent of total coral cover for scleractinian coral and hydrocoral species recorded in video surveys..................................................................................... 15 Table 3. A list of known scleractinian coral and hydrocoral species found within the Marine Conservation District. “X” indicates that the coral was found in this study and/or in the Territorial Coral Reef Monitoring Program. “o” indicates that these species were likely encountered in this study, but could not be distinguished in the field. .......................................................................................................... 17 Table 4. Species of scleractinian coral affected by unknown necrosis, and the prevalence, severity, and sample size among affected species in the Marine Conservation District. ......................................... 34 Table 5. Abundance, size and biomass of commercially important species of grouper and snappers in 80 fish transects from all habitat strata combined in the Marine Conservation District. ........................... 53 Table 6. Species observed during roving dives in the MCD at fixed sites (sampled 2005-2007) and on four designated habitat types sampled during the MCD survey. Fixed sites, RHB and CSE, are both coral reef habitat. Designated habitat types on the MCD survey include algae (A), coral (C), pavement (P) and sand (S). Abundance categories are (no fish), 1(one fish), 2 (2-10 fish), 3 (11-100 fish) and 4 (101-1000 fish)..................................................................................................................................... 54

III

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

LIST OF FIGURES Figure 1. Map of the wider Caribbean (top panel), the shelf area of the Puerto Rican Shelf, U.S. Caribbean, with the Red Hind Marine Conservation District (MCD) indicated (yellow polygon) (middle panel), bathymetry of the MCD (lower panel). Permanent coral reef monitoring locations are College Shoal East (CSE) and Red Hind Bank (RHB). ...................................................................................... 6 Figure 2. A) MCD multibeam bathymetry data at 1m resolution, inset shows bathymetry detail. B) MCD habitat map indicating the four strata (algae, coral, pavement and sand) used in study design, inset shows habitat detail. Maps created with GIS using data provided by Moody 2003 and Prada 2003. . 7 Figure 3. Representative photos of distinct habitat types sampled within the Marine Conservation District and Corresponding to A) Algae, B) Coral, (following page) C) Pavement, and D) Sand. ................... 13 Figure 4. Percent coverage of A) all coral species and B) Montastraea annularis species complex in the Marine Conservation District................................................................................................................ 16 Figure 5. Percent coverage of A) gorgonians and B) sponges in the Marine Conservation District.......... 18 Figure 6. Percent coverage of A) macroalgae, B) Schizothrix spp., (following pages) C) Lobophora variegata, D) Dictyota spp., E) dead coral covered with turf algae, and F) crustose coralline algae in the Marine Conservation District.......................................................................................................... 19 Figure 7. Percent coverage of A) all non-living substrata, (following page) B) sand/sediment, C) pavement, and D) rubble in the Marine Conservation District. ............................................................ 21 Figure 8. Depths of sampling locations among four predicted strata of the Marine Conservation District. Figure components are mean (thick black line), median (thin black line), 25th and 75th percentiles (bottom and top of box, respectively), 10th and 90th percentiles (bottom and top whiskers, respectively) and values outside 10th and 90th percentile (dots). Homogeneous subsets of means are indicated with letters. ........................................................................................................................... 23 Figure 9. The percent coverage (±SE) of the benthic fauna categories coral, gorgonians, and sponges among the predicted benthic strata (N = 80). Homogeneous subsets of means are indicated with letters (Tukey HSD Post Hoc comparison). ......................................................................................... 25 Figure 10. . The percent coverage (±SE) of the benthic algae categories total algae, total macroalgae, the filamentous cyanobacteria Schizothrix spp., and the phaephyte Lobophora variegata among the predicted benthic strata (N = 80). Homogeneous subsets of means are indicated with letters (Tukey HSD Post Hoc comparison)................................................................................................................. 25 Figure 11. The percent coverage (±SE) of the benthic algae categories the phaephyte Dictyota spp., dead coral covered with turf algae, and crustose coralline algea among the predicted benthic strata (N = 80). Homogeneous subsets of means are indicated with letters (Tukey HSD Post Hoc comparison).26 Figure 12. The percent coverage (±SE) of the abiotic benthic categories total non-living substrata, sand/sediment, pavement, and rubble among the predicted benthic strata (N = 80). Homogeneous subsets of means are indicated with letters (Tukey HSD Post Hoc comparison). .............................. 26 IV

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District Figure 13. Proportionate benthic composition determined from in situ surveys across four general benthic strata determined from side-scan sonar processing: algae, coral, pavement, sand. In situ data within each benthic strata were apportioned into hard substrate, soft substrate, and variable substrate categories with proportion of total (%) in brackets............................................................................... 30 Figure 14. Prevalence (yellow circle) and severity (green diamond) of A) total disease, (following page) B) unknown necrosis, C) white plague, and D) bleaching on hard corals of the Marine Conservation District. ................................................................................................................................................. 31 Figure 15. Prevalence and severity of A) recent partial mortality and B) old partial mortality on hard corals in the Marine Conservation District...................................................................................................... 33 Figure 16. Photographic examples of coral disease in the Marine Conservation District: A) a coral colony affected by white plague (site: coral 58), B) corals affected by unknown necrosis. (site: Pavement 119, October 9, 2007). ................................................................................................................................. 35 Figure 17. Photographic examples of coral disease in the Marine Conservation District: A) intecostal necrosis B) close-up of intercostal necrosis (site: pavement 111, October 29, 2007). ....................... 36 Figure 18. Photographic examples of coral disease in the Marine Conservation District: A) intercostals necrosis grading to general necrosis B) close-up of intercostal necrosis grading to general necrosis (site: pavement 111, October 9, 2007) ................................................................................................ 37 Figure 19. Mean species richness (±SE) for each habitat strata in the Marine Conservation District...... 39 Figure 20. Family richness of A) Scaridae, B) Serranidae, C) Lutjanidae, and D) Haemulidae at each sampling location in the Marine Conservation District......................................................................... 40 Figure 21. Total number of the most abundant species observed in belt transects in each habitat strata of the Marine Conservation District. Species were included if cumulative number in all habitats was ≥ 5 and then ordered from the most to least abundant from the coral habitat........................................... 42 Figure 22. Abundance (ind. 100m-²) of commercially important species (E. guttatus, M. venenosa, E. striatus, L. analis, O. chrysurus and B. vetula) at each sampling location in the Marine Conservation District. ................................................................................................................................................. 43 Figure 23. Family abundance (ind. 100m-²) of A) Acanthuridae, B) Scaridae, C) Haemulidae, D) Serranidae, E) Lutjanidae, and F) Balistidae at each sampling location in the Marine Conservation District. ................................................................................................................................................. 44 Figure 24. E. guttatus A) abundance (ind. /100m²) and B) biomass (g/100m²) at each sampling location in the Marine Conservation District.......................................................................................................... 46 Figure 25. Mean biomass (SE bars) for all fish at each sampling strata in the Marine Conservation District ............................................................................................................................................................. 47 Figure 26. Family biomass (g/100m²) of A) Acanthuridae, (following pages) B) Scaridae, C) Haemulidae, D) Serranidae, E) Lutjanidae, and F) Balistidae at each sampling location in the Marine Conservation District. ................................................................................................................................................. 47

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District Figure 27. . Trophic level abundance (ind. /100m²) of A) herbivores, B) invertivores, C) spongivores, D) piscivores, E) planktivores, and F) omnivores at each sampling location in the Marine Conservation District. ................................................................................................................................................. 50 Figure 28. Length frequency histograms for selected commercially important families (Acanthuridae and Scaridae) and species (O. chrysurus, E. guttatus and B. vetula) in all habitat strata combined in the Marine Conservation District................................................................................................................ 52

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

LIST OF APPENDICES Appendix I Sampling locations in the Marine Conservation District and their general characteristics.... 65 Appendix II Electronic supplement (DVD). Doted images used in benthic composition assessments. . 67 Appendix III Electronic supplement (DVD). Video captures of each sampling location. ........................ 67 Appendix IV Video mosaic images of representative coral reef locations from the strata coral (Coral 52) and sand (Sand 166). Scaling quadrat is 25 X 25 cm. Video mosaic analysis courtesy of A. Gleason and P. Reid (RSMAS-University of Miami). ......................................................................................... 68 Appendix V Electronic supplement (DVD). Benthic cover data for each location sampled in the Marine Conservation District............................................................................................................................ 70 Appendix VI Electronic supplement (DVD). Coral health data for coral harboring locations sampled in the Marine Conservation District................................................................................................................ 70 Appendix VII Electronic supplement (DVD). Fish data for each location sampled in the Marine Conservation District............................................................................................................................ 70 Appendix VIII Literature Review: literature pertinent to the Marine Conservation District ...................... 70

VII

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

EXECUTIVE SUMMARY The University of the Virgin Islands, in collaboration with the Caribbean Fisheries Management Council, completed a survey of the habitat and fisheries resources of the Red Hind Marine Conservation District (MCD), St Thomas, United States Virgin Islands. The purpose of this research was to validate habitat classifications developed for the CFMC and to assess fisheries and non-fisheries resources within this marine protected area. This research provides information that is applicable for the classification and ranking of essential fish habitat (EFH) within the MCD and similar mesophotic reef habitat (30 – 50 m) along the Puerto Rican Shelf. Benthic habitat assessments revealed extensive and well developed mesophotic coral reefs at depths of 34 – 47 m. Coral reefs were determined to occupy 65% of sites sampled in the MCD (coral cover > 4%, N = 80), with an average coral coverage of 25.3% ± 2.1 SE and maximum coral coverage of 50.1%. Coral species richness was high, with 37 species or genera recorded from the MCD, including the threatened elkhorn coral (Acropora cervicornis). Coral coverage was dominated (91.8%) by members of the Montastraea annularis species complex. Benthic habitats were predicted with variable accuracy using classified sonar imagery. Coral reefs were found to occupy almost all sampling strata predicted to contain pavement and sand habitat types. However, algae and coral strata were well predicted. Coral health assessments revealed an extensive and severe cryptic coral mortality event caused by an unknown disease referred to as unknown necrosis. Disease signs and mortality covered a coherent region comprising over one fifth of the area of the MCD. The mean prevalence (42.4% ± 6.3 SE, N = 27) and severity (32.8% ± 4.6 SE) of unknown necrosis at affected sites suggested that the effects of the disease were intense in these areas, and may have contributed to a loss of over half the coral coverage. Motile resource surveys of fish and commercially important invertebrates showed a total of 112 fish species. No motile macro invertebrates were seen during our surveys, but previous studies within the MCD have documented spiny lobster (Panulirus argus) and channel crabs (Mithrax spinosissimus). Species richness and biomass were highest in coral habitats followed by sand and pavement. Species richness was significantly lower in algal habitats than in the three other habitat types. Fish assemblage structure was dominated numerically by herbivorous and planktivorous species. Greatest fish biomass was found among invertivores, herbivores, and piscivores, respectively. Commercially important species were found primarily in coral and hard-bottom habitats. The fact that fish communities in the sand stratum were similar to coral and pavement strata indicates the discrepancy in habitat classification. True sand habitats contained a fish assemblage structure more similar to algal plains. Abundance and distribution of reef

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

fishes varied throughout the MCD depending upon taxa and presence of habitat types within the MCD. For example, the queen triggerfish (Balistes vetula) showed higher biomass near the northern boundary and in the eastern end of the MCD. These portions of the MCD contained large, sandy areas that are the preferred habitat of B. vetula.

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

INTRODUCTION The Sustainable Fisheries Act of 1996 provided for significant changes in the management of fishery resources. In particular, it created the concept of essential fish habitat (EFH) and required that scientific research be undertaken to determine habitats that were critically important to maintain fish stocks. Defined as “those waters and substrate necessary to fish for spawning, breeding, feeding or growth to maturity” this amendment to the Magnuson-Stevens Act requires a broader assessment of the habitats and locations that should be afforded protection. To address threats of over fishing and provide additional protective measures to essential fish habitat, the Caribbean Fishery Management Council (CFMC) initiated a study of one of the most significant marine protected areas within the US Virgin Islands, the Red Hind Bank Marine Conservation District (MCD; Fig. 1, Fig. 2). Established in 1999, regulations within the MCD prohibit anchoring and fishing of any kind, except trolling for pelagic species (Federal Register 64:213). The MCD encompasses 44.5 square kilometers (39.5 square kilometer < 50 m depth) of federal waters at the edge of the Puerto Rican Shelf south of St. Thomas. Although a number of studies have been conducted within the MCD (Beets and Friedlander 1999, Nemeth 2004, Whiteman et al 2005, Nemeth and Quandt 2005, Herzlieb et al. 2006, Smith et al. 2008) there remain significant gaps in knowledge about the fish and benthic resources within its boundaries. For example, little is known about deepwater shelf slope and shelf edge benthic communities nor the status of the fish stocks that utilize these habitats. Characterization and assessment of benthic habitats and associated communities will provide valuable data to inform the development and/or revision of fishery management plans based on the principles expressed by the EFH concept. This information can be used to understand the necessity of future marine protected areas and guide their designation. The specific objectives of the research presented in this report were to characterize benthic habitat composition across the MCD, provide ground validation to support existing GIS habitat maps of the MCD, assess the health of coral resources, and quantitatively describe the associated fish and fisheries resources. This report fills in gaps of previous research within the MCD and provides baseline characterization data that will allow greater assessment and management of EFH within the MCD and across similar mesophotic reefs within the U.S. Caribbean.

Background Studies conducted in the MCD have indicated that the area includes numerous deep habitats (30-100+ m) that contain resources important to fisheries dependent economies and regional biodiversity. A detailed

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

bathymetric and habitat characterization of the MCD was conducted in 2003 (Moody 2003; Prada 2003, Rivera et al. 2006) using multibeam sonar and Side Scan Sonar (SSS) imagery (Fig.1, Fig. 2). These studies delineated three meta-communities including corals and gorgonians, submerged aquatic vegetation, and sand. Within these meta-communities 23 habitat types were classified. Direct visual surveys of sessile benthic communities within the MCD have suggested the presence of exceptionally rich and extensive mesophotic coral reef communities. Autonomous underwater vehicle (AUV) benthic surveys (Armstrong et al. 2006) and in situ assessments and monitoring (Nemeth et al. 2005, Herzlieb et al. 2006, Smith et al. 2008a) have revealed coral reef banks and patch reefs dominated by reef forming corals of the Montastraea annularis species complex. These surveys have also shown that coral cover is typically higher (10-50%) and coral health greater than on shallow and midshelf reefs in the USVI (Herzlieb et al. 2006, Smith et al. 2008) and wider Caribbean (Gardner et al. 2003). Coral cover and health at two locations within the MCD (Hind Bank and College Shoal East) have been under semi-annual to annual benthic monitoring since 2003 by the USVI Territorial Monitoring Program (Figure 1, lower panel; Nemeth et al. 2006, Smith et al. 2007). These detailed studies of benthic composition and trajectory, and coral health, have shown that reefs within the MCD may be partially buffered from the effects of climate change through mechanisms that include reduced light intensity and moderation of temperature as the result of upwelling along the shelf edge (Smith et al. 2008b, in prep.). Such studies suggest that the MCD may be a regionally important refuge for coral reef biodiversity and fish habitat under scenarios of future seawater warming in the Western Atlantic (Donner et al. 2007). The MCD is likely to protect a large biomass of resident and transient commercially important fishes. Fish and fisheries resources in the MCD are broadly similar to shallow waters of the Puerto Rican Shelf, but differ from shallow and midshelf coral reefs in relative species composition and the occurrence of more rare deep water associated fishes (Nemeth et al. 2006). An important red hind (Epinephelus guttatus) spawning aggregation site (SPAG) within the MCD is well characterized (Olsen and Laplace 1979, Beets and Friedlander 1999, Nemeth 2005) and was the initial stimulus for establishment of the management area along the shelf edge. Historical SPAGs of the federally protected Nassau grouper (Epinephelus striatus) and existing or extant spawning aggregations of other commercially important species are also known from the MCD (Olsen and LaPlace 1979, Nemeth unpub data, Table 1). In addition, the Grammanik Bank, a 1.4 km reef less than 5 km east of the MCD boundary, hosts spawning aggregations of Nassau, yellowfin (Mycteroperca venenosa), yellowmouth (M. interstitialis) and tiger (M. tigris) grouper (Nemeth et al. 2006b). Recent hydro-acoustic data from Nassau and yellowfin grouper tagged on the Grammanik Bank indicate that many move west into the MCD between monthly spawning events in February, March and April, and that the MCD affords protection to those species from fishing during a very critical period of the year (Nemeth et al. 2008).

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Table 1. Species known to spawn or form aggregations around the red hind spawning aggregation site (18.202 N, 65.002 W). Common Name

Scientific Name

Number observed

Timing

Red hind Nassau grouper Tiger grouper Mutton snapper Schoolmaster snapper

Epinephelus guttatus E. striatus Mycteroperca tigris Lutjanus analis L. apodus

80,000 Historic 100 200 100

Dec-Feb Dec-Feb Jan-Mar Mar-May Mar-May

Horse-eye jack Black jack

Caranx latus Caranx lugubris

300 500

Feb-Apr April

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

RHB

CSE

Figure 1. Map of the wider Caribbean (top panel), the shelf area of the Puerto Rican Shelf, U.S. Caribbean, with the Red Hind Marine Conservation District (MCD) indicated (yellow polygon) (middle panel), bathymetry of the MCD (lower panel). Permanent coral reef monitoring locations are College Shoal East (CSE) and Red Hind Bank (RHB).

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Figure 2. A) MCD multibeam bathymetry data at 1m resolution, inset shows bathymetry detail. B) MCD habitat map indicating the four strata (algae, coral, pavement and sand) used in study design, inset shows habitat detail. Maps created with GIS using data provided by Moody 2003 and Prada 2003.

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

METHODS Habitat Stratification and Sampling Design The ecosystem-based assessment of the Red Hind Marine Conservation District (MCD) presented in this report was based on a stratified random sampling strategy. Stratified random assessments offer a robust appraisal of natural resources with minimized bias (Menza et al. 2006). They allow for statistical assessment of sampling sufficiency for variables of interest and are amenable to predictive scaling of data, such as fish biomass and benthic composition, to larger geographic regions (Pittman et al. 2007). Assignment of sampling sites within the MCD was based on predicted habitat structure defined in GIS products produced by Prada 2003 (Figure 1B). Habitat designations by Prada (2003) were determined using modifications of the scheme produced by NOAA for shallow waters of Puerto Rico and U.S. Virgin Islands (NOAA-NOS 2001). Four predicted habitat designations were chosen for sampling and included: coral, pavement, sand, and algae. These designations encompassed all benthic areas of the MCD less than 50 m. The choice of these four general habitat strata, as opposed to more specific habitat designations that were available from GIS products was two-fold. First, because of uncertainties in true habitat composition from the assignment algorithms developed for shallow-water Caribbean benthos, it was decided that broad categories offered the greatest possibility of locating in situ assessments within accurately predicted habitats. Second, because of limitations in diver-based sampling effort in deep (30-50 m) benthic areas (i.e., 10 – 25 min. safe repetitive bottom times and the allocated study budget), it was predetermined that diver pairs could accomplish a robust number of surveys per stratum by using only four strata (80 total surveys). With these constraints, and with the lack of a priori information on habitat structure and motile resources, it was predicted that 20 surveys per predicted habitat strata would be required to make reliable assessments. Allocation of sampling effort within each predicted benthic strata was accomplished by randomized assignment of sampling locations using the Geographic Information System (GIS). Using the four predicted benthic habitat strata supplied in GIS format, gridlines were removed and all adjacent polygons were aggregated using the Dissolve tool in ArcGIS 9.2 (ESRI software) and clipped to remove habitat defined outside the MCD boundary. To exclude benthic sampling units smaller than the size of sampling surveys (25 m linear distance, see below), any polygons in the resulting file that had areas less than 625 m2 were removed from the shapefile. Hawth’s Tools extension for ArcGIS was than used to generate 200 random points, with 50 points allocated for each habitat type. Generated sampling points (locations) were constrained so that points could not lie within 25 meters of any other sampling point. This was done to avoid the possibility of re-sampling. Sampling units were also allocated to a maximum depth of 50 m, which was considered the maximum safe depth for repetitive dives following the sampling protocol. Sampling sites and their general characteristics are presented in Appendix I.

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

In Situ Sampling Protocols Randomly determined points were used a survey locations for in situ sampling protocols. Within each of the 50 random sampling points, the first 20 points were used as the drop point for diver pairs. Subsequent sampling points (randomly generated points 21-50) in each habitat strata were sequentially used if shipboard depth sounder measurements or initial diver reconnaissance of the sampling point determined that benthic areas were unsafe for completion of sampling protocols. This resulted in four randomly replaced surveys. Diver pairs utilizing technical NITROX or closed circuit rebreather were launched on the water surface within ten meters of the designated sampling point using ship-board GPS. Divers descended directly downwards on the sampling point. It was estimated that actual benthic sampling areas deviated no further than 25 meters from the predetermined sampling point of each survey. The sampling protocol was designed to assess benthic composition, coral health, and the abundance of fish and other motile resources. Upon reaching the seafloor diver pairs deployed 30 m transect line along a random compass direction determined a priori using the function RAND()*360 in Microsoft Excel. Each diver pair had divided responsibility for resource assessments. Diver 1 deployed the transect tape while assessing fish and other motile resources. Following Diver 1, Diver 2 assessed the health of coral resources along the transect line. After Diver 1 had deployed the transect tape, Diver 1 returned along the transect line and recorded the benthos using digital video. Detailed methods for assessment of benthic composition, coral health, fish, and other motile resources are described below.

Benthic Composition Benthic composition was recorded along a transect using standardized video monitoring protocols (Aronson et al. 1994, Carleton and Done 1995, Rogers and Miller 2001, Rogers et al. 2001). The video monitoring protocol is used in numerous coral reef benthic sampling programs, including the USVI Territorial Coral Reef Monitoring Program (Nemeth et al. 2006a, Smith et al. 2007). This protocol maximizes assessment times underwater and is particularly useful in time-limited assessments, such as in deep diving conditions. During video sampling, a diver swam at a uniform speed (~5 min. per transect) recording the benthic cover using a Sony TRV-950 digital camcorder in a Light and Motion Stingray II underwater housing. The diver pointed the camera down and perpendicular to the substrata. A guide wand attached to the camera housing was used to help the diver maintain the camera at a constant distance of approximately 0.4 m above the bottom as they followed the vertical contour of the substratum. The total length of the transect taped by the diver was variable but ranged between a minimum of 10 m to a max of 30 m. In habitats that the diver determined were homogenous (e.g., unbroken coral cover, algal plane), the diver recorded the terminal 10 m of the transect. In habitats that were more variable (e.g., reef edge habitats and other mixed bottom habitats), the diver recorded the full 30 m of the transect. This

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

strategy maximized limited bottom time when the habitat could be sufficiently sampled in a short transect, but captured greater variability in heterogeneous sampling locations. After taping, approximately 40 - 80 non-overlapping images per transect were captured and saved as JPEG files on a computer using a Sony video capture card. Captured images represented a planar area of reef that varied around a true planar area of 0.31 m2 (0.64 m x 0.48 m), and ultimately depended on the rugosity of the substratum. Microsoft Excel and Adobe Photoshop were used to superimpose ten randomly located dots on each captured image. The benthic cover under each of the points was then identified by experienced observers (TCRMP) to the lowest identifiable taxonomic level or abiotic group. For each transect, the percent cover of benthic categories was calculated by dividing the number of random points falling on the substrata type by the total number of points for the transect. The benthic categories that were assessed included: coral, dead coral with turf algae, macroalgae, sponges, gorgonians, and non-living substrata (sand, sediment, rubble, pavement). A total of 4,403 images were analyzed across the study. Mean values for percent cover were calculated for each site and values were arcsine transformed prior to analysis. Data were tested for normality and homogeneity of variance. Data that met assumptions were analyzed with one-way ANOVA between benthic strata (algae, coral, pavement, non-living). Data that did not meet assumptions were tested with a non-parametric Wilcoxon test on rank sums. Significantly different means were analyzed post hoc with Tukey’s HSD tests.

Coral Health Scleractinian coral and hydrocoral colonies (all sizes) located directly under the transect lines were assessed in situ for signs of mortality and disease following a modified Atlantic and Gulf Rapid Reef Assessment protocol (AGRRA; Kramer et al. 2005). The line intercept method of coral health assessment provided a total coral sample size of 1,233 colonies for the study. Partial mortality of coral colonies was broken into the categories ‘old partial mortality’, skeleton eroded and covered with turf or macroalgae, and ‘recent partial mortality’, skeleton not eroded (fine corallite structure still intact) and bare or with a thin veneer of sheeting or filamentous algae. In the USVI, the transition between recent and old mortality categories usually occurs within three months following tissue death (Smith pers. obs.). In addition, old mortality becomes unrecognizable when the colony erodes into an amorphous form or a coral secretes new skeleton away from the dead surface. At this point it difficult to discern if a new coral has settled on a dead colony and sheeted, or if a surviving portion of a partially dead colony has resheeted. This transition takes place between 1 – 4 years after the initial mortality (Smith pers. obs.; also see http://www.agrra.org/method/methodcor.html). The surface area (%) of the colony that was dead was also estimated for each partial mortality category. Disease lesions and signs were categorized into recognized Caribbean scleractinian diseases and syndromes (e.g., white plague) following Bruckner 2007. In addition

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

to recognized coral diseases, a novel coral disease was encountered across numerous sites and is referred to in this study as Unknown Necrosis. The severity of disease on coral colonies was estimated as the area (percent of colony) of active disease lesion. Bleaching1 was assessed as abnormal paling of the colony, and, when present, the severity of the bleaching (paling or total whitening) and the area of the colony affected were assessed. This data was used to ordinate bleaching intensity into one of five categories: 0) unbleached, 1) any degree of paling less than completely white, or 1% - 10% bleached, 2) 10% – 50% bleached, and 4) 50% – 90% bleached, and 4) >90% bleached (after Gleason 1993). For each transect at each location, the prevalence of colonies with mortality, bleaching, and disease was calculated by dividing the number of affected colonies by the total number of colonies assessed.

Fish and Other Commercially Important Motile Resources Characterization of the fish community and motile invertebrates (primarily lobster) was conducted along 25 x 4 m belt transects used in the benthic habitat surveys. Within this belt transect all fish species and motile invertebrates were identified to species level and total length estimated in 5 cm and 10 cm size categories (i.e. <5, 6-10, 11-20, 21-30, 31-40, >40). Data from fish and motile invertebrate density, diversity and size distribution were analyzed using non-parametric statistics (because of non-normal distribution patterns) to test for differences among habitat strata within the MCD. Detection of the less abundant and often more commercially valuable species may be limited using standard belt transects. Therefore, a timed swim (i.e. roving diver technique) was conducted at a subset of randomly selected sites in which two divers actively searched for rare, cryptic and highly mobile species as well as invertebrate mega-fauna. Divers remained within a single habitat type but indicated on their data sheet if searches included habitat edges (abrupt change from one habitat type to another) or gradual transitions. The duration of the timed swim was constant throughout the study across all sites and habitats and was determined based on appropriate dive times and safety protocol. During the timed swim the observer recorded to species and enumerated all fish and invertebrate mega-fauna encountered on a logarithmic scale (i.e. 1, 2-10, 11-100, 101-1000). Data for total fish diversity was obtained from roving dives in the MCD at four designated habitat types (algae, coral, pavement, sand) sampled during the MCD survey and at two fixed sites sampled from 2005-2007 (see Table 6). These two sites included the Red Hind Bank (RHB), a coral reef site located in the eastern end of the MCD, and Collage Shoal East (CSE), another coral site located in the western part of the MCD (Figure 1, lower panel).

Bleaching is presented separately from other disease due to differing assessment of severity.

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

RESULTS AND RECOMMENDATIONS Benthic Composition Benthic sampling revealed a unique array of habitat types ranging from topographically simple algal and sand planes to highly complex coral reef banks. Examples of these habitats are presented as photographs in Fig. 3 (A-D), in captured stills from video transects (Appendix II), as videos (Appendix Video Captures III) and as video mosaics (Appendix III). The benthic composition of sampling locations is presented as percent cover from video assessments in Fig 4 - 7.

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Figure 3. Representative photos of distinct habitat types sampled within the Marine Conservation District and Corresponding to A) Algae, B) Coral, (following page) C) Pavement, and D) Sand.

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District C

Figure 3. (continued)

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Scleractinian Corals and Hydrocorals Coral cover had a mean of 16.6% ± 1.9 SE and ranged from 0 to 50.1% across the MCD (Fig. 4A). For all locations that had coral cover greater than zero, coral cover had a mean of 20.7% ± 2.1 SE (N = 64). The dominant coral across the MCD were members of the Montastraea annularis species complex [Montastraea annularis (Ellis & Solander, 1786), Montastraea faveolata (Ellis and Solander, 1976), and Montastraea franksi Gregory, 1895)] and their coverage largely drove overall coral cover trends (Fig. 4B, Table 2). The cover of the M. annularis species complex across the MCD had a mean of 14.9% ± 1.8 SE and ranged from 0 to 48.1%. In total, 20 scleractinian coral species or genera and one hydrocoral species were identified in video transects (Table 2). This estimate of scleractinian coral species richness was revised upwards to 25 from in situ coral health assessments that tended to include small and cryptic coral species that were missed in video analysis and permitted a greater ability to separate rare genera into species (Table 3). At present, the number of known scleractinian coral and hydrocoral species for the MCD is 37 (Table 3), including one threatened species [Acropora cervicornis (Lamarck, 1816)].

Table 2. The ranked percent coverage and percent of total coral cover for scleractinian coral and hydrocoral species recorded in video surveys.

Species Montastraea annularis species complex Agaricia spp. (Lamarck, 1801) Porites astreoides (Lamarck, 1816) Agaricia agaricites (Linnaeus, 1758) Montastraea cavernosa (Linnaeus, 1767) Siderastrea siderea (Ellis and Solander, 1786) Porites porites (Pallas, 1776) Stephanocoenia intersepta (Lamarck, 1816) Diploria labyrinthiformis (Linnaeus, 1758) Mycetophyllia spp. (Milne Edwards and Haime, 1848) Madracis formosa (Wells, 1973) Colpophyllia natans (Houttyn, 1772) Siderastrea radians (Pallas, 1766) Diploria strigosa (Dana, 1848) Madracis decactis (Lyman, 1859) Agaricia undata (Ellis & Solander, 1786) Millepora alcicornis (Linnaeus, 1758) Mycetophyllia aliciae (Wells, 1973) Manicina areolata (Linnaeus, 1758) Mycetophyllia ferox (Wells, 1973) Eusmilia fastigiata (Pallas, 1766)

Benthic Coverage 14.81% 0.47% 0.36% 0.28% 0.21% 0.08% 0.05% 0.04% 0.02% 0.02% 0.01% 0.01% 0.01% 0.01% 0.01% 0.01% 0.01% 0.01% 0.004% 0.003% 0.002%

Percent of Coral Cover 91.8% 2.9% 2.3% 1.7% 1.3% 0.5% 0.3% 0.2% 0.1% 0.1% 0.1% 0.1% 0.1% 0.1% 0.1% 0.04% 0.03% 0.03% 0.02% 0.02% 0.01%

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

%Coverage Coral

%Coverage Montastraea annularis species complex

Figure 4. Percent coverage of A) all coral species and B) Montastraea annularis species complex in the Marine Conservation District.

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Table 3. A list of known scleractinian coral and hydrocoral species found within the Marine Conservation District. “X” indicates that the coral was found in this study and/or in the Territorial Coral Reef Monitoring Program. “o” indicates that these species were likely encountered in this study, but could not be distinguished in the field. Found in Present

Found in

Study

TCRMP

Species

Acropora cervicornis X

Agaricia agaricites Agaricia fragilis Dana, 1848

X X

Agaricia grahamae Wells, 1973

Agaricia humilis Verrill, 1901

X X

Agaricia lamarcki Milne Edwards and Haime, 1851

Agaricia undata

Colpophyllia natans

Dichocoenia stokesii Milne Edwards and Haime, 1851

X X X

Diploria labyrithiformis

Diploria strigosa (Dana, 1848)

Eusmilia fastigiata

Isophyllia sinuosa (Ellis & Solander, 1786)

Helioseris cucullata (Ellis & Solander, 1786)

Madracis decactis

Madracis formosa

Madracis mirabilis (Duchassaing & Michelotti, 1860)

X X

Mancinia aerolata Meandrina meandrites (Linnaeus, 1767)

Montastraea annularis

Montastraea cavernosa

Montastraea faveolata

Montastraea franksi

Millepora alcicornis

Millepora complanata Lamarck, 1816

Mycetophyllia ferox

Mycetophyllia aliciae

Mycetophyllia danaana Milne Edwards and Haime, 1848

Mycetophyllia lamarckiana Milne Edwards and Haime, 1851

Oculina diffusa Lamarck, 1816

Porites astreoides

Porites divaricata Lesueur, 1821

Porites porites

Scolymia spp. Haime, 1852

Siderestrea siderea

Siderestrea radians

Stephanocoenia intercepta

X X X

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Gorgonians and Sponges Gorgonians and sponges were minor constituents of the benthos of the MCD, and separately averaged less than 1% of benthic coverage (Fig. 5). The coverage of soft corals had a mean of 0.1% ± 0.02 SE and ranged from 0 to 1.1%. The coverage of sponges had a mean of 0.7% ± 0.1 SE and ranged from 0 to 3.9%. Although species-level discrimination of soft corals and sponges was not attempted from video transects, observations suggested that diversity within these groups was high and included rare and commercially important shallow water types, such as antipatharians (black coral). Soft coral and sponge diversity can be more accurately assessed with surveys that directly target these groups, use methods to determine area-based density, and attempt in situ species level categorization. A.

%Coverage Gorgonians

%Coverage Sponges

Figure 5. Percent coverage of A) gorgonians and B) sponges in the Marine Conservation District.

Algae Algae covered the majority of the substrata for most sites in the MCD. Algal cover had a mean of 61.6% ± 1.7 SE and ranged from 0 to 94.8%. The majority of algal cover consisted of macroalgae and large filamentous cyanobacteria, which together had a mean coverage of 38.6 ± 2.3 SE and ranged from 0 to 93.3% (Fig. 6A). Over half of macroalgae and filamentous cyanobacteria were composed of the

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

filamentous cyanobacteria Schizothrix spp. (mean = 11.7% ± 1.9 SE, range: 0 to 70.5%; Fig. 6B) and the phaeophyte Lobophora variegata (mean = 9.7% ± 1.0 SE, range: 0 to 30.1%; Fig. 6C). A minor component of macroalgae was composed of Dictyota spp., (mean = 0.5% ± 0.1 SE; Fig. 6D). Dead coral covered with turf algae comprised the second most important category of algae and had a mean of 18.9% ± 1.8 SE and ranged from 0 to 64.7% (Fig. 6E). Crustose coralline algae formed a relatively minor component of the benthic algal cover and had a mean of 4.1% ± 0.5 SE and ranged from 0 to 16.7% (Fig 6F).

%Coverage Macroalgae

%Coverage Schizothrix spp.

Figure 6. Percent coverage of A) macroalgae, B) Schizothrix spp., (following pages) C) Lobophora variegata, D) Dictyota spp., E) dead coral covered with turf algae, and F) crustose coralline algae in the Marine Conservation District.

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

%Coverage Lobophora variegata

%Coverage Dictyota spp.

%Coverage Dead Coral with Turf Algae

Figure 6. (continued)

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

%Coverage Crustose Coralline Algae

Figure 6. (continued)

Non-living Benthic Cover Non-living benthic substrata formed the third highest benthic coverage category after algae and corals, and had a mean of 20.8% ± 2.3 SE and ranged from 0 to 97.5% (Fig. 7A). The majority of non-living substrata was composed of sand/sediment, and had a mean coverage of 15.7% ± 2.2 SE and ranged from 0 to 97.2% (Fig. 7B). Pavement formed the second highest portion of non-living substrata, and pavement had a mean coverage of 3.1% ± 0.6 SE and ranged from 0 to 27.5% (Fig. 7C). Coral rubble formed the third highest portion of non-living substrata, and had a mean coverage of 2.1% ± 0.7 SE and ranged from 0 to 38.5% (Fig 7D).

%Coverage Non-living Substrata

Figure 7. Percent coverage of A) all non-living substrata, (following page) B) sand/sediment, C) pavement, and D) rubble in the Marine Conservation District.

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

%Coverage Sand/Sediment

%Coverage Pavement

%Coverage Rubble

Figure 7. (continued)

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Benthic Composition by Sampling Strata The four predicted benthic strata used to stratify sampling effort had different characteristics for many of the examined variables. Depth was significantly different between strata (F = 8.5, p < 0.0001, N = 80), with the coral stratum significantly shallower than all other strata, which were not different from each other (Fig. 8).

Figure 8. Depths of sampling locations among four predicted strata of the Marine Conservation District. Figure components are mean (thick black line), median (thin black line), 25th and 75th percentiles (bottom and top of box, respectively), 10th and 90th percentiles (bottom and top whiskers, respectively) and values outside 10th and 90th percentile (dots). Homogeneous subsets of means are indicated with letters.

Benthic Epifauna Coral coverage was significantly different between strata (χ2 = 55.1, p < 0.0001). The coral stratum had significantly higher coral coverage than all other strata (nearly double), with the pavement and sand strata not significantly different from each other, but higher than the algae stratum, which was nearly zero (Fig. 9). Gorgonian coverage was significantly different between strata (χ2 = 17.8, p < 0.0005), and was highest in the coral and pavement strata, and over an order of magnitude less in the sand and algae strata (Fig. 9). However, pavement was not significantly different than the higher coverage coral stratum or the lower coverage sand and algae strata. Sponge coverage was significantly different between strata (χ2 = 16.0, p

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

< 0.0012), but gradually declined from the coral stratum, to the pavement and sand strata, and finally to the algae strata (Fig. 9). Only the coral and algae strata were significantly different from each other.

Algae Algae coverage was significantly different between strata (χ2 = 16.7, p < 0.0008). The coral stratum had significantly less algal cover than the pavement and sand strata, but was not significantly different from the algae stratum (Fig. 10). The cover of macroalgae was significantly different between strata (χ2 = 22.3, p < 0.0001). The coral stratum had the least cover of macroalgae and was significantly different from the algae stratum, which had the highest macroalgae cover (Fig. 10). Coverage of macroalgae in the sand and pavement strata was intermediate between the coral and algae strata. Sub-categories of macroalgae cover were only significantly different for L. variegata (F = 9.4, p < 0.0001). The coverage of L. variegata was highest in the coral and pavement strata, which were not significantly different from each other (Fig 10). Coverage of L. variegata was least in the algae stratum, and intermediate in the sand stratum. Other sub-categories of macroalgae cover were not significantly different between strata, and included Schizothrix spp. (χ2 = 2.7, p = 0.438) and Dictyota spp. (F = 2.0, p = 0.122). The remaining two categories of algal cover did show differences between strata. Dead coral covered with turf algae was significantly different between strata (χ2 = 38.4, p < 0.0001). The coverage of dead coral covered with turf algae was significantly lower in the algae stratum than all other strata, and was an order of magnitude less in the algae stratum than in the other strata (Fig. 11). The coverage of dead coral covered with turf algae was not significantly different between the coral, pavement, and sand strata. Crustose coralline algae coverage was significantly different between strata (χ2 = 32.0, p < 0.0001). The coral and the pavement strata had the highest coverage of crustose coralline algae and were not significantly different from each other (Fig. 11). The coral and pavement strata had coverage of crustose coralline algae that were significantly greater than the algae stratum, but not the sand stratum, which was intermediate between the coral and pavement strata, and the algae stratum.

Non-living Benthic Composition Non-living benthic coverage was significantly different between strata (χ2 = 35.5, p < 0.0001). Non-living benthic coverage was significantly greater in the algae stratum than all other strata, which were not significantly different from each other (Fig. 12). Sand/sediment, the dominant component of non-living strata (see Benthic Composition Section), was significantly different between strata (χ2 = 37.3, p < 0.0001). Sand/sediment coverage was significantly greater in the algae stratum than all other strata, which were not significantly different from each other (Fig. 12). Pavement coverage was significantly different between strata (χ2 = 22.8, p < 0.0001). Pavement coverage in the pavement stratum was significantly greater than the algae and sand strata, which were not significantly different from each other (Fig. 12). Pavement coverage in the coral stratum was intermediate between the other strata. Rubble coverage was not significantly different between strata (F = 1.9, p = 0.132; Fig. 12). 24

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Figure 9. The percent coverage (±SE) of the benthic fauna categories coral, gorgonians, and sponges among the predicted benthic strata (N = 80). Homogeneous subsets of means are indicated with letters (Tukey HSD Post Hoc comparison).

Figure 10. . The percent coverage (±SE) of the benthic algae categories total algae, total macroalgae, the filamentous cyanobacteria Schizothrix spp., and the phaephyte Lobophora variegata among the predicted benthic strata (N = 80). Homogeneous subsets of means are indicated with letters (Tukey HSD Post Hoc comparison).

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Figure 11. The percent coverage (±SE) of the benthic algae categories the phaephyte Dictyota spp., dead coral covered with turf algae, and crustose coralline algea among the predicted benthic strata (N = 80). Homogeneous subsets of means are indicated with letters (Tukey HSD Post Hoc comparison).

Figure 12. The percent coverage (±SE) of the abiotic benthic categories total non-living substrata, sand/sediment, pavement, and rubble among the predicted benthic strata (N = 80). Homogeneous subsets of means are indicated with letters (Tukey HSD Post Hoc comparison).

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Predicted Versus Sampled Benthic Strata The randomized stratified sampling design allows an assessment of the accuracy of multibeam and sidescan sonar benthic habitat characterization in the MCD. There was uncertainty in what proportionate coverage of various groups (e.g., macroalgae, pavement, coral) represented the four predicted benthic categories: algae, coral, pavement, sand. This makes it somewhat difficult to compare the results of this study with the predicted benthic habitat. However, it was assumed that large deviations from predicted habitat structure would indicate obvious areas requiring improvement in the benthic classification algorithms. The percent composition of algae, coral, pavement, and sand strata are shown in Fig. 13. The predicted strata are broken into their constituent parts and represented as proportions of the total benthic coverage. In addition, the proportion of substrata that fell into hard substrata, soft substrata, or variable substrata is shown. Variable substrata are living benthic components (e.g., macroalgae, sponges) that are overlaying hard substrata (e.g., coral, dead coral with turf algae) or soft substrata (i.e., sand/sediment). Both in situ diver records and benthic cover reported in surveys deviate from predicted habitat types in many surveys. This problem was particularly evident in the pavement and sand strata. Each of the four predicted strata is assessed below:

Algae The algae stratum was consistent and well predicted. Sites classified as algae were low relief algal communities atop unconsolidated sediment (Fig. 3A). Although 55% of the algae stratum had variable benthic composition, in situ and video observation strongly suggested that this largely overlaid unconsolidated sediment. Thus, about 92% of algae stratum was unconsolidated sediment, of which 55% was composed of macroalgae and filamentous cyanobacteria, and 37% was composed of sand/sediment. In these areas, expanses of unconsolidated sediment were broken by occasional coral colonies and dead coral rubble piles, and much less commonly by patch coral reefs and pavement. Site-attached fishes were often associated with these small habitat patches.

Coral The coral stratum was consistent and well predicted. Sites classified as coral were medium to high relief coral reef. Although 28% of the coral stratum had variable benthic composition, in situ observation strongly suggested that this largely overlaid consolidated hardbottom. Thus, about 95% of the coral strata were composed of consolidated hardbottom, of which 38.7% ± 2.9 SE was composed of scleractinian corals. This was unusually high coral cover compared to modern shallow Caribbean coral reefs (~10% coral

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

cover; Gardener et al. 2003), and was particularly striking following coral mortality in shallow and deep coral reefs of the U.S. Caribbean following bleaching and disease in 2005 and 2006 (Smith et al. 2008, in prep.). In situ observations also suggested that the coral stratum could be further divided into sub-types associated with edges, shallow coral bank tops (30-40 m), and deeper coral plains (35 – 45 m). In all cases the dominant matrix was formed of low or plating morphologies of the M. annularis species complex (Fig 3B, Appendix IV). Living upper surfaces of corals often rested upon pillars of dead coral, creating a complex interstitial network of overhangs, channels, and tunnels. This under-explored network may form a large habitat area that is likely to be an important component of essential fish habitat in the MCD.

Pavement The pavement stratum was not well predicted. Sites classified as pavement were dominantly composed of coral reef that fell into the coral reef sub-categories of shallow coral reef bank tops, and deeper coral plains (see ‘coral’ section above). The pavement stratum had 31% variable benthic composition, and in situ observation suggested that this was largely composed of consolidated hardbottom. However, in the deeper coral plains there were patches of sand interspersed between coral pillars. Thus, for the shallower (30 – 37 m) coral reef bank tops found in the southwestern corner of the MCD (Fig. 2B), about 95% of the benthos was composed of consolidated hardbottom. In the deeper coral plains that ran as a channel through the center of the western MCD (Fig. 2B), there was higher variability in the variable substratum category, and it was estimated that consolidated hardbottom comprised about 80% of the benthos. As with the coral stratum, the matrix of the pavement stratum was dominantly composed of low or plating morphologies of the M. annularis species complex (Fig 3C). Living upper surfaces of corals often rested upon pillars of dead coral, creating a complex interstitial network of overhangs, channels, and tunnels; however, this network was more open than in the coral strata, and had fewer tunnels. Corals of this stratum may undergo periodic mortality events that generate a more open network. Sampling in the pavement strata revealed a widespread and severe coral mortality event, particularly evident and general to the deeper coral plain (see ‘Coral Health’ section below).

Sand The sand stratum was predicted to varying accuracy that depended on location in the MCD. Sites classified as sand were either sand/algae habitats or were coral reef. Accurately classified sand stratum sites were largely confined to the southeast corner of the MCD (Fig. 2B). In situ observations suggest that sand habitat classified outside the shelf edge coral reef banks at the south drop-off were largely soft

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

bottom habitats to a depth of at least 65 m. The remainder of habitat classified as sand was formed in a complex area of coral pillars and hillocks interspersed with sand patches (Fig. 3D). This area comprised a large section of the western MCD, behind the primary and secondary shelf edge coral reef banks (Fig. 2B). The sand stratum had 44% variable benthic composition, and in situ observation suggested that in the majority of habitat in the western MCD this was composed of an equal mix of consolidated hardbottom and unconsolidated sediments. Thus, for the coral reef hillocks found in the southwestern corner of the MCD, approximately 60% of the benthos was composed of consolidated hardbottom, and the remaining 40% of unconsolidated sediments (i.e. sand/sediment).. The majority of the sand stratum was comprised of coral hillocks that differed from the geomorphology of coral reefs in the coral and pavement strata. Instead of more horizontally uniform coral reef areas, the hillocks rose 2 to 7 m above the surrounding coral reef plain and were 5 to 15 m in diameter (Fig. 3D). The hillocks and the surrounding reef were dominantly composed of low or plating morphologies of the M. annularis species complex. Living upper surfaces of corals often rested upon pillars of dead coral, creating a complex interstitial network of overhangs, channels, and tunnels; however, as in the pavement stratum, this network was more open than in the coral stratum, and had fewer tunnels.

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Algae Soft (37%)

Coral Soft (4%)

Hard (8%)

Variable (55%)

Hard (68%)

Variable (28%)

Pavement Soft (5%)

Sand Soft (16%)

Hard (64%)

Variable (31%)

Hard (40%)

Variable (44%)

Hard Substrate

Soft Substrate

Variable Substrate

M. annularis species complex Other Corals

Sand/Sediment

Soft Corals Sponges Schizothrix spp. L. variegata

Dead Coral covered with Turf Algae Crustose Coralline Algae Pavement Rubble

Dictyota spp. Other Macroalgae

Figure 13. Proportionate benthic composition determined from in situ surveys across four general benthic strata determined from side-scan sonar processing: algae, coral, pavement, sand. In situ data within each benthic strata were apportioned into hard substrate, soft substrate, and variable substrate categories with proportion of total (%) in brackets.

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Coral Health Disease and Bleaching Assessments of coral condition showed wide variations of coral health across sites and striking recent disease and degradation in a spatially coherent area of the MCD. Coral health was assessed on 1,233 colonies across 64 locations that contained coral within transects. Overall, coral disease (with the exception of bleaching) on coral colonies had a mean prevalence of 18.5% ± 0.03 SE and a mean severity (percent of colony affected) of 26.9% ± 3.9 SE (Fig. 14A). The vast majority of coral disease signs were caused by an unknown coral syndrome, named “unknown necrosis” (Fig. 14B). Unknown necrosis had a large mean prevalence of 17.4% ± 3.6 SE and mean severity of 32.8% ± 4.7 SE. This syndrome is described in further detail below. White Plague was the second most common disease, and had a mean prevalence of 0.8% ± 0.004 SE and a mean severity of 11.6% ± 3.2 SE (Fig. 14C). Bleaching had a mean prevalence of 12.4% ± 1.5 SE and a mean severity (ordinated levels) of 1.9% ± 0.1 SE (Fig. 14D). This severity most closely corresponds to level 2 bleaching: 10% – 50% coral colony bleached.

Disease Prevalence and Severity x

Figure 14. Prevalence (yellow circle) and severity (green diamond) of A) total disease, (following page) B) unknown necrosis, C) white plague, and D) bleaching on hard corals of the Marine Conservation District.

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Figure 14. (continued) B.

Unknown Necrosis Prevalence and Severity

White Plague Prevalence and Severity

Bleaching Prevalence and Severity x

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Partial Mortality Partial mortality of coral colonies was a common feature of coral reefs within the MCD. Recent partial mortality had mean prevalence of 30.6% ± 4.0 SE and mean severity of 22.7% ± 2.9 SE (Fig. 15A). The prevalence of recent partial mortality was strongly correlated with the prevalence of unknown necrosis (R = 0.852, F = 171.1, p < 0.0001). Old partial mortality had a mean prevalence of 65.0% ± 3.4 SE and a mean severity of 26.2% ± 1.8 SE (Fig. 15B). The prevalence of old partial mortality was a general characteristic of most coral harboring sites and was not significantly correlated with the prevalence of unknown necrosis (R = 0.223, F = 3.4, p = 0.070).

Recent Partial Mortality Prevalence and Severity x

Old Partial Mortality Prevalence and Severity x

Figure 15. Prevalence and severity of A) recent partial mortality and B) old partial mortality on hard corals in the Marine Conservation District.

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Unknown Necrosis Coral disease sampling revealed an unusual, extensive, and dramatic syndrome affecting large numbers of coral. This unknown necrosis was manifested as white areas of tissue loss in a variable pattern. It was distinguished from white plague disease signs in that the areas of tissue loss were not present as a progressing linear lesion over colonies (Fig. 16). Instead, white areas of tissue necrosis appeared sporadically or generally over colonies. In members of the M. annularis species complex, presumably early stages of the disease primarily affected intercostal regions between the polyps (Fig. 17). These areas formed more general regions of necrosis in later stages of the disease (Fig. 18). Although the coral disease monitoring protocol was not longitudinal (individual colonies were not reassessed for pattern of disease progression), the progression of the disease was deduced from colonies displaying both sporadic intercostal necrosis and regional necrosis (Fig. 18). In coral species with meandering corallite structure (e.g., agariciids), signs of disease appeared as both tattered-appearing areas of necrosis and regional necrosis. Numerous colonies in affected areas were noticed that had recently suffered 100% mortality and 26 recently killed colonies were found in transects (2% prevalence). The disease was noted affecting three coral genera, however, prevalence was highest for the M. annularis species complex (Table 4).

Table 4. Species of scleractinian coral affected by unknown necrosis, and the prevalence, severity, and sample size among affected species in the Marine Conservation District. Species

Prevalence

Severity

Agaricia agaricites

0.020

Agaricia spp.

0.023

65%

Montastraea annularis species complex

0.203

40%

907

Siderastrea radians

0.167

50%

Siderastrea siderea

0.029

80%

Repeated observations and surveys of coral health in coral reefs affected by unknown necrosis suggested that that the disease peaked during earlier stages of sampling (October 2007) and had largely abated within three months. There was a high prevalence and severity of unknown necrosis in October 2007 and repeated observation after the start of 2008 revealed few cases of unknown necrosis. Furthermore, two locations sampled in October 2007 had a mean prevalence of unknown necrosis of 35.8%, but had no cases of unknown necrosis in January 2008. The abatement of unknown necrosis is also illustrated in the video mosaic image of location S166 recorded in January 2007 (Appendix IV – Sand 166). No visible signs of unknown necrosis are visible on this image, although unknown necrosis was present in October 2007.

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Figure 16. Photographic examples of coral disease in the Marine Conservation District: A) a coral colony affected by white plague (site: coral 58), B) corals affected by unknown necrosis. (site: Pavement 119, October 9, 2007).

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Figure 17. Photographic examples of coral disease in the Marine Conservation District: A) intecostal necrosis B) close-up of intercostal necrosis (site: pavement 111, October 29, 2007).

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Figure 18. Photographic examples of coral disease in the Marine Conservation District: A) intercostals necrosis grading to general necrosis B) close-up of intercostal necrosis grading to general necrosis (site: pavement 111, October 9, 2007)

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

The coral disease signs consistent with unknown necrosis were present over a large swath of the MCD. The occurrence of unknown necrosis on coral reefs was largely confined to the western-central area of the MCD in a basin behind the thin primary and wide secondary outer reef banks forming the southwestern corner of the MCD (see Fig. 14B). Observations and surveys of coral reefs adjacent to affected areas, but on topographic highs, such edges of reef banks or reef bank tops, showed that unknown necrosis was largely confined to the basin. In addition, the spatial structure of the disease across the MCD was highly autocorrelated, with less than a 1% chance of a random clustering (Moran's I Index = 0.32, Z Score = 7.98 SD, p < 0.01). The proximity of unaffected faunas and the spatial coherence of affected areas suggested that unknown necrosis was not being driven by a pathogen, but was a response to a common abiotic driver. The effects and extent of unknown necrosis in the MCD suggest a strong structuring force of essential fish habitat (EFH) in this mesophotic reef system. The mean prevalence (42.4% ± 6.3 SE, N = 27) and severity (32.8% ± 4.6 SE) of unknown necrosis at affected sites suggested that the effects of the disease were intense in these areas. Extrapolation of the occurrence of unknown necrosis across the basin suggested that an area of approximately 9 km2 was affected by the mortality event, over a fifth of the benthic habitat of the MCD shallower than 50m. While the driver of this disease and coral mortality were unknown, it is possible that cryptic mortality events are a recurrent and structuring force in these habitats. Importantly, the mass disease occurred outside of the two Territorial Coral Reef Monitoring Program sites maintained in the MCD. This mass disease and mortality event was captured opportunistically by coincidence with this research project to assess habitats and resources of the MCD. The occurrence, effects, and extent of large-scale mortality events in mesophotic reef systems may be an important consideration for EFH. The novel mass disease event presented in this research report should be an impetus for expanded and more intensive monitoring or coral health in mesophotic reefs.

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Assemblage Structure of Fish and Motile Mega-Invertebrates The reef fish community structure at each sampling location in the Marine Conservation District is presented as density per 100 m2 from belt transects. Motile mega-invertebrates were not observed in this study.

Diversity, Abundance and Biomass of Reef Fishes A total of 112 species were recorded throughout the MCD (see Table 6). Average fish species richness along belt transects was highest in coral habitats (mean = 14.6 100 m-2, range = 7 to 25 spp), followed by sand habitats (mean = 12.5 spp 100 m-2, range = 5 to 25 spp), pavement (mean = 12.1 spp 100 m-2, range = 6 to 18 spp) and algal plains (mean = 6.3 spp 100 m-2, range = 1 to 18 spp) (Fig. 19). Based on belt transects, Scarids showed the highest level of species richness followed by Serranids, Lutjanids and Haemulids (Fig. 20 A - D). Species richness was significantly lower in algal habitats (p < 0.05) than in the three other habitat types (i.e. coral, pavement and sand). The distribution of the most abundant species among the sampling strata is presented in Fig. 21.

Figure 19. Mean species richness (±SE) for each habitat strata in the Marine Conservation District.

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Scaridae Richness

Serranidae Richness

Lutjanidae Richness

Figure 20. Family richness of A) Scaridae, B) Serranidae, C) Lutjanidae, and D) Haemulidae at each sampling location in the Marine Conservation District.

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Haemulidae Richness

Figure 20. (continued)

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Figure 21. Total number of the most abundant species observed in belt transects in each habitat strata of the Marine Conservation District. Species were included if cumulative number in all habitats was ≥ 5 and then ordered from the most to least abundant from the coral habitat.

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Abundance of reef fishes varied throughout the MCD depending upon taxa. Most commercially important species were commonly found in coral reefs and hard bottom habitats (Fig. 22), with higher concentrations near habitat edges and greater abundance near the shelf edge, but also along the northern and western MCD boundaries. Species with the highest abundances that were also of high economic and ecological importance were used to plot abundance distribution patterns. Acanthurids (A. bahianus, A. coeruleus, A. chirurgus) were fairly evenly distributed throughout the MCD with slightly lower abundances near the northern boundary of the closed area (Fig. 23A). The Scarids had similar distribution patterns as the Acanthrids (Fig. 23B) but were 4 times more abundant. Haemulids were uncommon within the MCD and were present at less than 25% of the 80 sampling locations (Fig. 23C). Serranid abundance was relatively evenly distributed across the MCD (Fig. 23D) and the group was largely composed of smaller species within the Hypoplectrus and Serranus genera (see Table 6). Lutjanids had a similar distribution as Haemulids, but were observed at just over 50% of the sampling sites (Fig. 23E). Balistids were more common on the eastern end of the MCD and were present at nearly 25% of the sites (Fig. 23F). The red hind (Epinephelus guttatus) was present at nearly 60% of all sites and had relatively high biomass levels (Fig. 24).

Commercial Species Abundance

Figure 22. Abundance (ind. 100m-²) of commercially important species (E. guttatus, M. venenosa, E. striatus, L. analis, O. chrysurus and B. vetula) at each sampling location in the Marine Conservation District.

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Acanthuridae Abundance

Scaridae Abundance

Haemulidae Abundance

Figure 23. Family abundance (ind. 100m-²) of A) Acanthuridae, B) Scaridae, C) Haemulidae, D) Serranidae, E) Lutjanidae, and F) Balistidae at each sampling location in the Marine Conservation District.

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Serranidae Abundance

Lutjanidae Abundance

Balistidae Abundance

Figure 23. (continued)

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

E. guttatus Abundance

E.guttatus Biomass

Figure 24. E. guttatus A) abundance (ind. /100m²) and B) biomass (g/100m²) at each sampling location in the Marine Conservation District. Fish biomass along belt transects, which ranged from <1 to >60,000 g 100m-2, was highest in coral reef habitats, followed by sand, pavement and algal plains (Fig. 25). Due to high variability among transects no significant differences were found among habitat strata. The high biomass in sandy areas was partly due to the presence of significant coral cover that seemed to concentrate fish in these areas. At the family level, the biomass of Acanthurids was fairly uniform throughout sampling sites (Fig. 26A), whereas Scarid biomass biomass was more variable with respect to location (Fig. 26B). Haemulid biomass was relatively high at one coral reef site near the northern boundary of the MCD (Fig. 26C) and Lutjanid biomass was high at several sites located on coral reef and hardbottom habitats (Fig. 26D). Alternatively Balistid biomass was high near the northern boundary and eastern end of the MCD (Fig. 26F) in sand habitat. Serranid biomass was fairly uniform throughout MCD with one high biomass area near the shelf edge (Fig. 26D). Most of the Serranid biomass resulted from red hind being relatively abundant throughout the MCD (Fig. 24).

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Figure 25. Mean biomass (SE bars) for all fish at each sampling strata in the Marine Conservation District

Acanthuridae Biomass

Figure 26. Family biomass (g/100m²) of A) Acanthuridae, (following pages) B) Scaridae, C) Haemulidae, D) Serranidae, E) Lutjanidae, and F) Balistidae at each sampling location in the Marine Conservation District.

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Scaridae Biomass

Haemulidae Biomass

Serranidae Biomass

Figure 26. (continued)

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Lutjanidae Biomass

Balistidae Biomass

Figure 26. (continued)

Trophic composition among habitat strata Invertivores were the most common trophic guild and were found at all but one site, with the largest abundances along the southern shelf edge (Fig. 27B). These were followed in abundance by herbivores (Fig. 27A) and omnivores (Fig. 27F). The other three trophic guilds examined, spongivores, piscivores, and plankitovres, were similar in abundance (Fig. 27C - E). In general, biomass and richness within trophic guilds followed abundance patterns except for the invertevores and piscivores, which showed spatially distinct biomass patterns.

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Herbivore Abundance

Invertivore Abundance

Spongivore Abundance

Figure 27. . Trophic level abundance (ind. /100m²) of A) herbivores, B) invertivores, C) spongivores, D) piscivores, E) planktivores, and F) omnivores at each sampling location in the Marine Conservation District.

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Piscivore Abundance

Planktivore Abundance

Omnivore Abundance

Figure 27. (continued)

Size structure of Reef Fishes Size structures of selected commercially important species or species groups are shown in (Fig. 28). The herbivorous Acanthurids and Scarids had very similar size distributions, which ranged from 5 to 30 cm total 51

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

length and averaged 10.7 cm TL and 11.0 cm TL, respectively (Fig. 28). The two most common Scarids were Scarus taeneopterus and S. iserti, which, when combined, were approximately three times more abundant than the three common Acanthurid species. The planktivorous yellowtail snapper (Ocyurus chrysurus) averaged 23.8 cm FL and ranged from 5 – 40 cm. Red hind (E. guttatus) averaged 31.4 cm TL and the queen triggerfish (B. vetula) averaged 32.4 cm FL. Both species had a similar size distribution (Fig. 28). The larger and commercially important species of grouper and snapper were rarely counted along transects within the MCD (Table 5).

Acanthuridae

Scaridae

O. chrysurus

E. guttatus

B. vetula

Figure 28. Length frequency histograms for selected commercially important families (Acanthuridae and Scaridae) and species (O. chrysurus, E. guttatus and B. vetula) in all habitat strata combined in the Marine Conservation District..

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Table 5. Abundance, size and biomass of commercially important species of grouper and snappers in 80 fish transects from all habitat strata combined in the Marine Conservation District. Species

Size (cm TL) 80-100

(g/100m²)

0-20

20-40

34.9

Mycteroperca venenosa 2

32.2

M. tigris

58.7

M. interstitialis

35.0

Lutjanus analis

159.6

L. cyanopterus

426.9

L. jocu

236.7

Lachnolaimus maximus

122.6

Epinephelus striatus

40-60 60-80

Total biomass

Assessment of total fish species diversity used a combination of approaches and reliance on previous fish surveys. The majority of fish diversity data was derived from the 25 x 4 m (100m2) belt transects that were conducted at each of the 80 sampling sites. An additional timed roving diver survey was conducted at one randomly selected site within each habitat strata. Because of the limited time available at each site due to depth constraints we could not conduct a timed roving survey at each site. We also included data that has been collected over several years during annual monitoring at the Hind Bank and College Shoal East study sites, fish collected during research sampling (i.e. fish traps and hook and line) on the red hind spawning aggregation site, and incidental observations during numerous dives within the MCD. The compilation of these data produced a fish list of 122 species within 35 families (Table 6). Many of these species are rare, transient pelagic fishes2 that form an important component of the trophic food web. Due to the depth and complexity of the various habitat strata it is recommended that future surveys include a larger roving diver survey component with a minimum of 60 min search time per site. A portion of these surveys should also include dives during the changeover period during sunrise or sunset to observe the many cryptic and nocturnal species that exist within the complex interstitial spaces of these deep mesophotic reefs.

This included an approximately 160 kg blue marlin (Makaira nigricans) that repeatedly charged one diver pair at a 5 m

decompression stop over site Sand 166 on October 8, 2007.

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Table 6. Species observed during roving dives in the MCD at fixed sites (sampled 2005-2007) and on four designated habitat types sampled during the MCD survey. Fixed sites, RHB and CSE, are both coral reef habitat. Designated habitat types on the MCD survey include algae (A), coral (C), pavement (P) and sand (S). Abundance categories are (no fish), 1(one fish), 2 (2-10 fish), 3 (11-100 fish) and 4 (101-1000 fish). Family Species

Common Name

Fixed Sites

MCD Survey

RHB

CSE

Acanthuridae Acanthurus bahianus Acanthurus chirurgus Acanthurus coeruleus

ocean surgeonfish doctorfish blue tang

3 . 2

3 2 2

. 2 .

2 . 2

3 2 3

1 2 .

Aulostomidae Aulostomus maculatus

trumpetfish

Balistidae Balistes vetula Canthidermis sufflamen Melichthys niger Xanthichthyes ringens

queen triggerfish ocean triggerfish black durgeon sargassum triggerfish

1 2 2 .

. 3 3 .

2 . . .

. . . .

2 . . .

1 . . 3

Carangidae Caranx crysos Caranx latus Caranx lugubris Caranx ruber Elagatis bipinnulata Seriola dumerili

blue runner horseeye jack black jack bar jack rainbow runner greater amberjack

. 2 2 1 . 1

1 2 3 1 1

. . . . .

. . . 2 .

. . . . .

Carcharhinida Negaprion brevirostris Galeocerdo cuvier Carcharhinus leucas Carcharhinus perezii

lemon shark tiger shark bull shark reef shark

2 1 1 1

Chaetodontidae Chaetodon aculeatus Chaetodon capistratus Chaetodon sedentarius Chaetodon striatus

longsnout butterflyfish foureye butterflyfish reef butterflyfish banded butterflyfish

2 2 2 2

Coryphaenidae Coryphaena hippurus

dolphinfish

Echeneidae Echeneis naucrates

sharksucker

. 2 1 . .

. . . .

. 3 1 2

. . . .

2 2 2 2

2 . . .

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Table 6. (continued) Family Species

Fixed Sites

Common Name

RHB

MCD Survey

CSE

Ephipphidae Chaetodipterus faber

spadefish

Exocoetidae Cheilopogon melanurus

Atlantic flyingfish

Gobiidae Coryphopterus dicrus Coryphopterus glaucofraenum

colon goby bridled goby

. .

. 2

. .

1 2

Grammatidae Gramma loreto

fairy basslet

Haemulidae Anisotremus surinamensis Anisotremus virginicus Haemulon album Haemulon aurolineatum Haemulon carbonarium Haemulon flavolineatum Haemulon macrostomum Haemulon parra Haemulon plumieri Haemulon sciurus

black margate porkfish white grunt tomtate caesar grunt French grunt Spanish grunt sailors choice white grunt bluestriped grunt

. 1 . . 2 2 0 . 1 2

. 2 . . . 1 1 . 2 1

. . . . . . . . . .

. . 2 3 . 2 . 1 1 1

1 2 . 3 1 2 1 1 . 2

. . . . . . . . . .

Holocentridae Holocentrus adscensionis Holocentrus coruscum Holocentrus marianus Holocentrus rufus Myripristis jacobus

squirrelfish reef squirrelfish longjaw squirrelfish longspine squirrelfish blackbar soldierfish

. . 2 2

2 . . . 1

. . . 2 2

2 1 1 . 3

. . . 2 .

. . . . .

Inermiidae Inermia vittata

boga

Istiophoridae Makaira nigricans

blue marlin

Kyphosidae Kyphosus spp.

chub

. 1

. 2

. .

1 .

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Table 6. (continued) Family Species

Fixed Sites

Common Name

MCD Survey

RHB

CSE

Labridae Bodianus rufus Clepticus parrae Halichoeres bivittatus Halichoeres garnoti Lachnolaimus maximus Thalassoma bifasciatum

Spanish hogfish creole wrasse slippery dick yellowhead wrasse hogfish bluehead wrasse

1 4 1 3 1 3

2 4 1 3 1 3

. . . 3 . .

2 . . 2 1 2

2 4 . . . .

. . 2 2 . 2

Lutjanidae Lutjanus analis Lutjanus apodus Lutjanus buccanella Lutjanus cyanopterus Lutjanus griseus Lutjanus jocu Ocyurus chrysurus

mutton snapper schoolmaster blackfin snapper cubera snapper gray snapper dog snapper yellowtail snapper

2 4 3 . 2 . 3

. 2

. .

2 3

. 1

1 .

Malacanthidae Malacanthus plumieri

sand tilefish

Mobulidae Manto birostris

manta ray

Mullidae Mulloidichthys martinicus Pseudupeneus maculatus

yellow goatfish striped goatfish

2 2

1 1

. 3

2 1

. 2

. .

Muraenidae Gymnothorax funebris Gymnothorax moringa

green moray spotted moray

2 .

. .

. 1

. .

Myliobatidae Aetobatus narinari

spotted eagle ray

Lactophyrs quadricornis Lactophyrs triqueter

scrawled cowfish smooth trunkfish

1 2

2 . 2 2

. . . .

. . 1 2

. . . 2

. . . .

. 1 1

. . .

. . 2

. .

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Table 6. (continued) Family Species

Fixed Sites

Common Name

MCD Survey

RHB

CSE

Pomacanthidae Centropyge argi Holacanthus ciliarus Holacanthus tricolor Pomacanthus arcuatus Pomacanthus paru

cherub fish queen angel rock beauty gray angelfish French angelfish

. 2 2 1 .

. 1 1 . 1

2 . . . .

. 2 2 . 2

. 2 1 2 2

1 . . . .

Pomacentridae Chromis cyanea Chromis multilineata Stegastes partitus Stegastes planifrons Stegastes variabilis

blue chromis brown chromis bicolor damselfish threespot damselfish cocoa damselfish

4 1 3 . .

4 4 3 . .

. . 2 . .

3 2 3 . 1

. . 3 2 .

. . 3 . .

Priacanthidae Priacanthus cruentatus

glasseye snapper

Rhincodontidae Ginglymostoma cirratum

nurse shark

Scaridae Scarus guacamaia Scarus iserti Scarus taeniopterus Scarus vetula Sparisoma aurofrenatum Sparisoma chrysopterum Sparisoma rubripinne Sparisoma viride

rainbow parrotfish striped parrotfish princess parrotfish queen parrotfish redband parrotfish redtail parrotfish redfin parrotfish stoplight parrotfish

1 2 2 3 2 . 2 2

. 1 3 2 1 2 . 3

Scombridae Scomberomorus regalis Scomberomorus cavalla Acanthocybium solandri

cero mackerel king mackerel wahoo

1 1 1

. . . . 2 . . .

2 . .

. 3 2 . 2 . . 2

. . .

. 3 3 1 3 . . 3

. . .

. . 1 . 2 . . .

. . .

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Table 6. (continued)

Family Species

Fixed Sites

Common Name

MCD Survey

RHB

CSE

1 1 . . . . . 1 . 1 1

. . 2 . . . . . . . .

. 2 2 1 2 1 1 . . . .

. 1 2 2 2 . . 0 . . .

. . 2 2 . . . . . . .

Serranidae Epinephelus striatus Epinephelus cruentatus Epinephelus fulvus Epinephelus guttatus Hypoplectrus chlorurus Hypoplectrus nigricans Hypoplectrus puella Hypoplectrus unicolor Mycteroperca interstitialis Mycteroperca tigris Mycteroperca venenosa Liopropoma rubre Paranthias furcifer Serranus baldwini Serranus tabacarius Serranus tigrinus Serranus tortugarum

Nassau grouper graysby coney red hind yellowtail hamlet black hamlet barred hamlet butter hamlet yellowmouth grouper tiger grouper yellowfin grouper peppermint basslet creole-fish lantern bass tobaccofish harlequin bass chalkfish

. 2 1 2 2 1 2 1 1 2 2 1 3 . 1 1 .

Sparidae Calamus spp.

porgy

Sphyraenidae Sphyraena barracuda Tetrodontidae Canthigaster rostrata

1 . . 1 .

. 3 2 2 2

. . . . .

. 4 2 2 .

great barracuda

sharpnose puffer

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

CONCLUSIONS AND RECOMMENDATIONS Improved Evaluation of EFH within the MCD •

Benthic habitat classification from sonar imagery was capable of distinguishing major blocks of habitat, but was less able to correctly allocate those blocks to the habitat they represented. This may be a problem of novel habitat types, such as low relief coral reef habitat types composed of plating corals, which are not represented in benthic habitat algorithms developed for shallow-water communities. A formal re-assessment of the of the benthic maps using the recently acquired in situ surveys should be a priority and will improve the next generation of habitat classification models for Caribbean mesophotic systems.

•

Based on the limited sampling within the 39.5 km2 of the MCD shallower than 50 m, we found a large diversity of reef fishes (112 species total) from all trophic groups. We anticipate that the number of species will continue to increase as sampling efforts expand to include more sites. The relationship between fish species richness and habitat type was only distinct between algae and all other habitats. The three other habitats (coral, pavement and sand), as classified in the original benthic mapping, had considerable overlap in habitat features. Specifically the sand habitat included up to 60% variable hard bottom, which contributed to a high degree of species richness. The influence of edges was also apparent. Many of the large piscivores were found along the transition between coral reef and pavement, sand and algal habitats. It is recommended that these transition zones between habitats be included as a separate classification in future biodiversity surveys.

•

Large areas of coral reefs within the MCD are dominated by extensive interstitial space created by holes, tunnels, channels and ledges. These areas likely contain a high diversity of invertebrates and cryptic fishes, may contain commercially important species, such as the Caribbean spiny lobster (Panularis argus), and likely serve as essential fish habitat. Effective surveys of these habitats could be accomplished with stationary visual censuses.

•

While most species represented were adults, juveniles and sub-adults of many species were also present. This was especially apparent in the herbivorous Scaridae as well as the omnivorous Labridae and the planktivorous Pomacentridae. These species may well form the base of the food web for the larger piscivores (groupers, snappers), which in turn provide food for the top predators (sharks). In the absence of fishing, we anticipate that this large deepwater ecosystem will begin to show significant changes in the trophic structure of reef fishes and subsequent resilience to natural perturbations. The protection of the red hind spawning aggregation has already resulted significant improvements in the red hind population, and has improved the fishery as well (Nemeth 2005). Potential spill-over of commercially important reef fishes from the MCD to the surrounding

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

areas is anticipated but needs to be studied to better quantify the benefits of this closure to the local fishery (see below). •

Considerable habitat within the MCD lies on the outer shelf edge at depths greater than 50m. Dives to 70 m along this margin revealed a unique foliose agariciid coral reef community that may extend linearly along the shelf edge in suitable depths. This area may be particularly important as a movement corridor and as habitat for commercially important species, such as Nassau and yellowfin grouper, and blackfin snapper (Authors, unpub. obs.). Surveys by scientific divers in these deeper habitats are feasible with recent improvements in the reliability of technical diving systems (closed circuit rebreathers).

•

Video methods offer minimum estimates of species richness for epifauna, with the exception of scleractinian corals. Better estimates of species richness and diversity could be obtained by in situ identification along belt transects or within quadrats.

•

Little is known about the seasonality of the benthos, and motile fish and invertebrate communities in the MCD, nor the processes (e.g., oceanographic forcing) that drive seasonality. More intensive longitudinal studies could resolve these patterns and processes

Expansion of the Evaluation of EFH Outside the MCD •

Evaluation of the effectiveness of the MCD in promoting and sustaining regional fisheries via spillover requires comparative surveys outside the MCD. This could help to assure stakeholders that the reserve is functioning to improve local economic and ecological condition, at the expense of access to traditional fishing grounds.

•

Comparative surveys outside the MCD would also serve to describe the extent of mesophotic reefs and associated species. These reef systems are probably widespread on the Puerto Rican Shelf and are likely critical fisheries areas.

•

Other federal marine protected areas in the U.S. Virgin Islands would benefit from EFH assessment. These include the Grammanik Bank, Lang Bank, and the Mutton Snapper closed areas. Comparative studies could be conducted between these three CFMC management areas, and might provide valuable insights into factors affecting successful management strategies and avenues for improved management.

•

In addition to closed areas, large areas of the Puerto Rican Shelf and the St. Croix shelf have recently been surveyed with high-resolution multibeam sonar by NOAA. In situ surveys in these areas could be useful, not only for expanding knowledge of mesophotic and mid-depth systems (see above), but also for validating improved benthic habitat classification and resource prediction models,

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

•

Comparative surveys outside the MCD would also help to determine the processes controlling the formation and degradation of mesophotic reefs. The capture of a severe cryptic disease/mortality event outside annual monitoring locations, and recent work by Menza et al. (2007), underscores the lack of knowledge of processes that shape these deeper coral reef environments. These assessments will be crucial to understanding the potential of mesophotic reef systems to serve as refugia during a period of increasing sea surface temperatures and increasing frequency of coral bleaching events.

ACKNOWLEDGEMENTS We would like to thank the following people and organizations. Additional funding to support fieldwork was provided by the National Oceanographic and Atmospheric Administration, National Ocean Sciences Biogeography Program through the USVI Division of Coastal Zone Management (Department of Planning and Natural Resources), and the US National Science Foundation through the USVI Experimental Program to Stimulate Competitive Research (USVI NSF-EPSCoR) and the Lana Vento Charitable Trust. Excellent administrative support was given by C. Joseph. We are also indebted to the field assistance and wisdom of K. Turbe, R. Garcia, M. Carlos, J. Sabater, and K. Brown. All views expressed are those of the authors and do not necessarily reflect the views of the granting agencies and support personnel. This is contribution #52 from the Center for Marine and Environmental Studies at the University of the Virgin Islands.

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

LITERATURE CITED Armstrong RA, Singh H, Torres J, Nemeth RS, Can A, Roman C, Eustice R, Riggs L, Garcia-Moliner G (2006) Characterizing the deep insular shelf coral reef habitat of the Hind Bank marine conservation district (US Virgin Islands) using the Seabed autonomous underwater vehicle. Continental Shelf Research 26:194-205 Aronson RB, Edmunds PJ, Precht WF, Swanson DW, Levitan DR (1994) Large-scale, long-term monitoring of Caribbean coral reefs: simple, quick, inexpensive techniques. Atoll Research Bulletin 421:1-19 Beets J, Friedlander A (1999) Evaluation of a conservation strategy: a spawning aggregation closure for red hind, Epinephelus guttatus, in the U.S. Virgin Islands. Environ Biol Fishes 55:91-98 Bruckner AW (2007) Field Guide to Western Atlantic Coral Diseases and Other Causes of Coral Mortality. NOAA, UNEP-WCMC, PADI Carleton JH, Done TJ (1995) Quantitative video sampling of coral reef benthos: Large-scale application. Coral Reefs 14:35-46 Donner S, Knutson T, Oppenheimer M (2007) Model-based assessment of the role of human-induced climate change in the 2005 Caribbean coral bleaching event. Proceedings of the National Academy of Science 104:5483-5488 Gardner TA, Côté IM, Gill JA, Grant A, Watkinson AR (2003) Long-term region-wide declines in Caribbean corals. Science 301:958-960 Herzlieb S, Kadison E, Blondeau J, Nemeth RS (2006) Comparative assessment of coral reef systems located along the insular platform south of St. Thomas, US Virgin Islands and the relative effects of natural and human impacts. Proc 10th Int Coral Reef Symp 4-2:1144-1151 Kramer P, Lang J, Marks K, Garza-Perez R, Ginsburg R (2005) AGRRA Methodology, version 4.0, June 2005. University of Miami, Miami Menza C, Ault J, Beets J, Bohnsack J, Caldow C, Christensen J, Friedlander A, Jeffrey C, Kendall M, Luo J, Monaco M, Smith S, Woody K (2006) A Guide to Monitoring Reef Fish in the National Park Service's South Florida/Caribbean Network. NOAA Technical Memorandum. NOS NCCOS 39 166pp. Menza C, Kendall M, Rogers C, Miller J (2007) A deep reef in deep trouble. Continental Shelf Research 27:2224-2230 Moody G (2003) Marine Habitat Mapping Offshore St. Thomas & St. Croix, U.S. Virgin Islands EEZ. Géophysique GPR International Inc., Montreal 30pp. Nemeth RS (2005) Population characteristics of a recovering US Virgin Islands red hind spawning aggregation following protection. Marine Ecology Progress Series 286:81-97

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Nemeth RS, Quandt A (2005) Differences in fish assemblage structure following the establishment of the Marine Conservation District, St. Thomas U. S. Virgin Islands, Vol 56. Proceedings of the 56th Gulf and Caribbean Fisheries Institute. Tortola, BVI.:367-381 Nemeth RS, Smith TB, Taylor M, Herzlieb S, Kadison E, Blondeau J, Carr L, Allen-Requa L (2006a) Coral Reef Monitoring in St. Croix and St. Thomas, United States Virgin Islands. Year Five Final Report. University of the Virgin Islands, St. Thomas 142pp. Nemeth RS, Kadison E, Herzlieb S, Blondeau J, Whiteman E (2006b) Status of a yellowfin grouper (Mycteroperca venenosa) spawning aggregation in the US Virgin Islands with notes on other species. Proc. 57th Gulf Carib Fish Inst. 57:543-558 Nemeth R, Blondeau J, Kadison E, Calnan J and Smith T (2008) Determining the appropriate size of closed areas for protecting spawning aggregations of large groupers using hydro-acoustics. 11th International Coral Reef Symposium, Ft. Lauderdale, Fl. Abstract NOAA-NOS (2001) Benthic Habitats of Puerto Rico and the U.S. Virgin Islands. National Oceanic and Atmospheric Administration Olsen D, LaPlace J (1978) A study of Virgin Islands grouper fishery based on a breeding aggregation. Proc 31st Gulf Caribb Fish Inst 31:130-144 Prada M (2003) Deliniation of Benthic Habitats from Side Scan Sonar mosaics from the Marine Conservation District, St. Thomas, Lang Bank and Mutton Snapper Closed Area in St. Croix, US Virgin Islands. Géophysique GPR International, Inc, Montreal 22pp. Pittman S, Christensen JD, Caldow C, Menza C, Monaco ME (2007) Predictive mapping of fish species richness across shallow-water seascapes in the Caribbean. Ecological Modelling 204:9-21 Rivera J, Prada M, Arsenault J-L, Moody G, Benoit N (2006) Detecting Fish Aggregations from Reef Habitats Mapped with High Resolution Side Scan Sonar Imagery. NOAA Professional Paper NMFS 5: 88-104 Rogers C, Miller J (2001) Coral bleaching, hurricane damage, and benthic cover on coral reefs in St. John, U.S. Virgin Islands: a comparison of surveys with the chain transect method and videography. Bulletin of Marine Science 69:459-470 Rogers CS, Garrison G, Grober R, Hillis Z, Franke M (2001) Coral Reef Monitoring Manual for the Caribbean and Western Atlantic. US National Park Service, St. John Smith TB, Nemeth RS, Calnan JM, Taylor M, Kadison ES, Blondeau J, Tyner E (2007) Coral Reef Monitoring in St. Croix and St. Thomas, United States Virgin Islands. Year Six Final Report. University of the Virgin Islands, St. Thomas 52pp. Smith T, Calnan J, Nemeth R, Carr L, Requa-Allen L, Kadison E, Blondeau J, Taylor M, Rothenberger J (in prep) Impacts of the 2005 Caribbean mass bleaching event in the U.S. Virgin Islands Smith TB, Nemeth R, Blondeau J, Calnan J, Kadison E, Herzlieb S (2008a) Assessing coral reef health across onshore to offshore stress gradients in the US Virgin Islands. Marine Pollution Bulletin doi:10.1016/j.marpolbul.2008.08.015

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Smith TB, Nemeth RS, Blondeau J, Calnan JM, Kadison E (2008b) Mesophotic reefs of the Puerto Rican Shelf: physical processes, resistance to climate change, cryptic mortality, and future trajectories 11th International Coral Reef Symposium, Ft. Lauderdale, Fl. Abstract. Whiteman EA, Jennings CA, Nemeth RS (2005) Sex structure and potential female fecundity in a red hind (Epinephelus guttatus) spawning aggregation: applying ultrasonic imaging. J Fish Biol 66:983-995

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

APPENDICES

Appendix I Sampling locations in the Marine Conservation District and their general characteristics. Depth

Date

Strata

Location

Lat

Long

(m)

Sampled

Algae

MCD A 1

18.21919744

-65.05030908

10/4/07

Algae

MCD A 10

18.21244379

-65.009961

12/5/07

Algae

MCD A 11

18.20610503

-64.98787572

49.7

12/7/07

Algae

MCD A 12

18.21506709

-64.98559179

12/5/07

Algae

MCD A 14

18.21329212

-64.9887041

10/29/07

Algae

MCD A 15

18.20710693

-65.02136999

11/28/07

Algae

MCD A 16

18.21925007

-65.04407546

10/4/07

Algae

MCD A 17

18.21949375

-64.98597395

11/29/07

Algae

MCD A 18

18.21494988

-65.01856664

12/5/07

Algae

MCD A 19

18.21925069

-65.04407546

10/29/07

Algae

MCD A 2

18.21679587

-64.98672594

10/24/07

Algae

MCD A 20

18.20824479

-65.01694758

45.2

12/17/07

Algae

MCD A 21

18.21248121

-65.02591478

12/7/07

Algae

MCD A 3

18.21329212

-64.9887041

12/6/07

Algae

MCD A 4

18.21309074

-64.99823373

43.3

11/29/07

Algae

MCD A 5

18.21702446

-65.01869904

40.6

10/25/07

Algae

MCD A 6

18.21644204

-65.00624863

41.8

11/28/07

Algae

MCD A 7

18.21230098

-65.00099196

10/25/07

Algae

MCD A 8

18.21870191

-65.00884722

41.5

11/28/07

Algae

MCD A 9

18.21268362

-65.02757053

10/29/07

Coral

MCD C 51

18.19190106

-65.08136049

11/6/07

Coral

MCD C 52

18.19680569

-65.07908419

38.5

12/4/07

Coral

MCD C 53

18.20295433

-65.09902033

12/4/07

Coral

MCD C 54

18.21140366

-64.99764917

10/25/07

Coral

MCD C 55

18.18369391

-65.088798

11/12/07

Coral

MCD C 56

18.20718602

-65.09472463

10/12/07

Coral

MCD C 57

18.21783608

-65.05311238

11/28/07

Coral

MCD C 58

18.2062122

-65.02601558

10/9/07

Coral

MCD C 59

18.2040901

-65.05618887

10/4/07

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Depth

Date

Strata

Location

Lat

Long

(m)

Sampled

Coral

MCD C 60

18.20317584

-65.01958624

11/28/07

Coral

MCD C 61

18.20133302

-65.05618714

12/5/07

Coral

MCD C 62

18.21579034

-65.08645536

12/5/07

Coral

MCD C 63

18.21983358

-65.07391212

12/5/07

Coral

MCD C 64

18.20857843

-65.09350606

10/26/07

Coral

MCD C 65

18.21953265

-65.07429684

11/7/07

Coral

MCD C 66

18.20143394

-65.05989331

10/4/07

Coral

MCD C 67

18.21345617

-64.99281415

10/24/07

Coral

MCD C 68

18.18867811

-65.07260203

12/4/07

Coral

MCD C 69

18.21625361

-65.0766646

11/7/07

Coral

MCD C 70

18.20476208

-64.99586234

10/15/07

Pavement

MCD P 101

18.21115147

-65.02349829

10/24/07

Pavement

MCD P 102

18.19062453

-65.08751514

38.5

12/4/07

Pavement

MCD P 103

18.21899776

-65.0990227

36.4

10/8/07

Pavement

MCD P 104

18.19062453

-65.08751514

10/5/07

Pavement

MCD P 105

18.21020925

-65.01042807

10/24/07

Pavement

MCD P 106

18.2085

-65.07838436

11/7/07

Pavement

MCD P 107

18.19091406

-65.0710052

10/15/07

Pavement

MCD P 108

18.2072325

-65.04598339

11/27/07

Pavement

MCD P 109

18.19091406

-65.0710052

10/4/08

Pavement

MCD P 110

18.21267149

-65.05171893

12/5/07

Pavement

MCD P 111

18.20883751

-65.04400212

10/21/07

Pavement

MCD P 112

18.20491757

-65.07700044

11/30/07

Pavement

MCD P 113

18.18449445

-65.07658781

11/6/07

Pavement

MCD P 114

18.18929168

-65.07357517

11/6/07

Pavement

MCD P 115

18.2076195

-65.05173277

11/27/07

Pavement

MCD P 116

18.19190106

-65.08136049

11/7/07

Pavement

MCD P 117

18.20974776

-65.07146111

10/30/07

Pavement

MCD P 118

18.20971644

-65.04337465

10/29/07

Pavement

MCD P 119

18.20781846

-65.07670851

10/9/07

Pavement

MCD P 120

18.20442269

-65.084038

11/7/07

Sand

MCD S 151

18.20310948

-64.99622517

10/15/07

Sand

MCD S 152

18.20289399

-65.0009099

10/15/07

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Depth

Date

Strata

Location

Lat

Long

(m)

Sampled

Sand

MCD S 153

18.19811209

-64.98942559

10/25/07

Sand

MCD S 154

18.2057528

-64.99717632

11/29/07

Sand

MCD S 155

18.21150359

-65.07562486

10/30/07

Sand

MCD S 157

18.1930068

-65.08909274

10/26/07

Sand

MCD S 158

18.19974027

-64.98898819

50.9

12/6/07

Sand

MCD S 159

18.20193636

-65.02400739

10/9/08

Sand

MCD S 160

18.18786959

-65.07122747

11/12/07

Sand

MCD S 161

18.19710077

-65.08721552

11/19/07

Sand

MCD S 163

18.19908495

-65.07949955

1/30/08

Sand

MCD S 164

18.2100459

-64.98351194

11/29/07

Sand

MCD S 165

18.19132967

-65.09198426

12/4/07

Sand

MCD S 166A

18.19939785

-65.08599282

10/8/07

Sand

MCD S 167

18.18146399

-65.08364693

11/12/07

Sand

MCD S 168

18.20763223

-65.0689303

10/9/07

Sand

MCD S 169

18.20093822

-65.02001809

12/6/07

Sand

MCD S 170

18.20065975

-65.08328427

1/11/08

Sand

MCD S 172

18.20143232

-65.07907089

44.2

12/17/07

Sand

MCD S 173

18.21532859

-65.05840878

42.4

12/17/07

Appendix II Electronic supplement (DVD). Doted images used in benthic composition assessments.

Appendix III Electronic supplement (DVD). Video captures of each sampling location.

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Appendix IV Video mosaic images of representative coral reef locations from the strata coral (Coral 52) and sand (Sand 166). Scaling quadrat is 25 X 25 cm. Video mosaic analysis courtesy of A. Gleason and P. Reid (RSMAS-University of Miami).

Coral 52. January 16, 2008

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

S166. January 11, 2008

Nemeth et al. 2008. Habitats and Resources of the Marine Conservation District

Appendix V Electronic supplement (DVD). Benthic cover data for each location sampled in the Marine Conservation District.

Appendix VI Electronic supplement (DVD). Coral health data for coral harboring locations sampled in the Marine Conservation District.

Appendix VII Electronic supplement (DVD). Fish data for each location sampled in the Marine Conservation District.

Appendix VIII Literature Review: literature pertinent to the Marine Conservation District

Marine Conservation District Habitat Project Literature Review

Aponte NE, Ballantine DL (2001) Depth distribution of algal species on the deep insular fore reef at Lee Stocking Island, Bahamas. Deep Sea Research Part I 48:2185-2194 Appeldoorn R (1985) Support for the assessment of deepwater resources and evaluation of passive gears around Puerto Rico and the Virgin Islands. Caribbean Fisheries Management Council 24 Armstrong RA (2007) Deep zooxanthelate coral reefs of the Puerto Rico: US Virgin Islands insular platform. Coral Reefs 26:945 Armstrong RA, Singh H, Torres J, Nemeth RS, Can A, Roman C, Eustice R, Riggs L, Garcia-Moliner G (2006) Characterizing the deep insular shelf coral reef habitat of the Hind Bank marine conservation district (US Virgin Islands) using the Seabed autonomous underwater vehicle. Continental Shelf Research 26:194-205 Armstrong R, Singh H, Torres J (2002) Benthic survey of insular slope coral reefs using the Seabed AUV. Backscatter 13:22-25 Asch RG, Turgeon DD (2003) Detection of gaps in the spatial coverage of coral reef monitoring projects in the US Caribbean and Gulf of Mexico. Revista de Biologia Tropical 51:127-140

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Bak RPM, Nieuwland G (1995) Long-term changes in coral communities along depth gradients over leeward reefs in the Netherlands Antilles. Bulletin of Marine Science 56:609-619 Bak R, Nieuwland G, Meesters E (2005) Coral reef crisis in deep and shallow reefs: 30 years of constancy and change in reefs of Curacao and Bonaire. Coral Reefs 24:475-479 Ballantine D, Aponte N (1995) Deep-water algal distribution in the Bahamas, West Atlantic. Journal of Phycology 31:10 Beets J, Friedlander AM (1997) Evaluation of the spawning aggregation closure for red hind (Epinephelus guttatus), St. Thomas, US Virgin Islands. Caribbean Fishery Management Council, San Juan, P.R. 17 Beets J, Friedlander A (1999) Evaluation of a conservation strategy: a spawning aggregation closure for red hind, Epinephelus guttatus, in the U.S. Virgin Islands. Environ Biol Fishes 55:91-98 Beets J, Rogers C (2000) Changes in fishery resources and reef fish assemblages in a Marine Protected Area in the US Virgin Islands: the need for a no take marine reserve. Proceeding of the 9th International Coral Reefs Symposium 1:449-454. Burton M, Brennen K, Munoz R, Parker Jr. R (2005) Preliminary evidence of increased spawning aggregations of mutton snapper (Lutjanus analis) at Riley's Hump two years after the establishment of the Tortugas South Ecological Reserve. Fishery Bulletin 103:404-410 Busby RF, Bright CV, Pruna A (1966) Ocean bottom reconnaissance off the east coast of Andros Island, Bahamas U.S. Navy Oceanographic Office Technical Reports 20390. U.S. Naval Oceanographic Office, Washington, D.C. Calnan JM, Smith TB, Nemeth RS, Kadison E, Blondeau J (2008) Coral disease prevalence and host susceptibility on mid-depth and deep reefs in the United States Virgin Islands. Revista de Biologia Tropical (in press) Colin PL (1974) Observations and collection of deep-reef fishes off the coasts of Jamaica and British Honduras (Belize) Marine Biology 24:29-38

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CFMC (1996) Regulatory amendments to the fishery management of the reef fishery of PR and the USVI concerning red hind spawning aggregation, including regulatory management review and environmental assessment. Federal Register, Vol. 61 No. 235, December 5, 1996. p. 64485-64486 Collazo JA (1980) Monitoring and assessment of commercial deepwater fishes at three locations near Puerto Rico. CODREMAR, Mayaguez, P.R. p. 37 Cote IM, Mosqueira I, Reynolds JD (2001) Effects of marine reserve characteristics on the protection of fish populations: a meta-analysis. Journal of Fish Biology 59:178-189 Culter JK, Ritchie KB, Earle SA, Guggenheim DE, Halley RB, Ciembronowicz KT, Hine AC, Jarrett BD, Locker SD, Jaap WC (2006) Pulley reef: a deep photosynthetic coral reef on the West Florida Shelf, USA. Coral Reefs 25:228-228 Dammann A (1969) Study of the fisheries potential of the Virgin Islands. Special Report. Virgin Islands Ecological Research Station, University of the Virgin Islands, Caribbean Research Institute 197 Davies GE (1982) A century of natural change in coral distribution at the Dry Tortugas: a comparison of reef maps from 1881 to 1976. Bulletin of Marine Science 32:608-623 Dayton PK, Sala E, Tegner MJ, Thrush S (2000) Marine reserves: Parks, baselines, and fishery enhancement. Bulletin of Marine Science 66:617-634 Dennis G, Hensley D, Colin PL, Kimmel J (2004) New records of marine fishes from the Puerto Rican plateau. Caribbean Journal of Marine Science 40:70-87 Evans R, Russ G, Kritzer J (2008) Batch fecundity of Lutjanus carponotatus (Lutjanidae) and implications of no-take marine reserves on the Great Barrier Reef, Australia. Coral Reefs 27:179-189 Franklin EC, Ault JS, Smith SG, Luo J, Meester GA, Diaz JM (2003) Benthic habitat mapping in the Tortugas region, Florida. Marine Geodesy 26:19-34 Fricke H, Meischner D (1985) Depth limits of Bermudan scleractinian corals: A submersible survey. Marine Biology. Berlin, Heidelberg 88:175-187 Garcia-Sais J, Castro R, Sabater J (2001) Coral reef communities from Natural Reserves in Puerto Rico: a baseline quantitative assessment for prospective monitoring programs. Vol. 1 - Cordillera de

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Fajardo, Guanica, Bahia de Mayaguez, Caja de Muertos. U.S. Coral Reef Initiative Program, NOAA-DNER p. 232 Garcia-Sais J, Castro R, Sabater J, Carlo M (2001) Coral reef communities from Natural Reserves in Puerto Rico: a baseline quantitative assessment for prospective monitoring programs. Vol.2 - La Parguera, Boqueron, Isla de Mona, Isla Desecheo. U.S. Coral Reef Initiative Program, NOAADNER p. 193 Garcia-Sais J, Castro R, Sabater J, Carlo M (2001) Coral reef communities from Natural Reserves in Puerto Rico: a baseline quantitative assessment for prospective monitoring programs. Vol 3 GuaGuayanilla, Ponce, Guayama, Arroyo. U.S. Coral Reef National Monitoring Program, NOAADNER p.108 Garcia-Sais J, Castro R, Sabater J, Carlo M (2001) Baseline characterization of coral reef and seagrass communities from Isla de Vieques, Puerto Rico U.S. Coral Reef National Monitoring Program. NOAA-DNER p.108 Garcia-Sais J, Appeldoorn R, Bruckner AW, Caldow C, Christensen JD, Lilyestrom M, Monaco ME, Sabater J, Williams E, Diaz E (2005) The State of Coral Reef Ecosystems of the Commonwealth of Puerto Rico. In: Waddell J (ed) The State of Coral Reef Ecosystems of the United States and Pacific Freely Associated States: NOAA Technical Memorandum NOS NCCOS 11. NOAA/NCCOS Center for Coastal Monitoring and Assessment's Biogeography Team. Silver Springs, MD. Garcia-Sais J, Castro R, Sabater J, Carlo M (2005) Inventory and atlas of corals and coral reefs from the U.S. Caribbean EEZ (Puerto Rico and the United States Virgin Islands). CFMC/NOAA p. 215 Gardner J, Mayer L, Clarke J, Keiner A (1998) High-resolution multibeam bathymetry of East and West Flower Gardens and Stetson Banks, Gulf of Mexico. Gulf of Mexico Science XVI:128 Gilnack ML (1987) Deep water coral reef algal turfs: Analysis of community structure and biomass. Masters thesis, Southeastern Massachusetts University, p 65 Ginsburg RN, Harris PM, Eberli GP, Swart PK (1991) The growth potential of a bypass margin, Great Bahama Bank. Journal of Sedimentary Petrology 61:976-987

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Glynn PW (1973) Aspects of ecology of coral reefs in the Western Atlantic region. In: Jones OA, Endean R (eds) Biology and Geology of Coral Reefs. Volume II: Biology 1. Academic Press, New York, pp 480 Graham NAJ, Evans RD, Russ GR (2003) The effects of marine reserve protection on the trophic relationships of reef fishes on the Great Barrier Reef. Environmental Conservation 30:200-208 Grober-Dunsmore R, Frazer TK, Beets J, Lindberg WJ, Zwick P, Funnicelli N (2008) Influence of landscape structure on reef fish assemblages. Landscape Ecology 23:37-53 Grober-Dunsmore R, Frazer TK, Lindberg WJ, Beets J (2007) Reef fish and habitat relationships in a Caribbean seascape: the importance of reef context. Coral Reefs 26:201-216 Halpern BS (2003) The impact of marine reserves: Do reserves work and does reserve size matter? Ecological Applications 13:S117-S137 Hamilton SL, Regetz J, Warner RR (2008) Postsettlement survival linked to larval life in a marine fish. Proceedings of the National Academy of Sciences. 105 (5): 1561-1566 Herzlieb S, Kadison E, Blondeau J, Nemeth RS (2005) Comparative assessment of coral reef systems located along the insular platform south of St. Thomas, US Virgin Islands and the relative effects of natural and human impacts. Proc 10th Int Coral Reef Symp 4-2:1144-1151. Hubbard DK, Scaturo D (1985) Growth rates of seven species of scleractinian corals from Cane Bay and Salt River, St. Croix, USVI. Bulletin of Marine Science 36:325-338 Johnson KG, Jackson JBC, Budd AF (2008) Caribbean reef development was independent of coral diversity over 28 million years. Science 319:1521-1523 Jones GP, McCormick MI, Srinivasan M, Eagle JV (2004) Coral decline threatens fish biodiversity in marine reserves. Proceedings of the National Academy of Sciences 101:8251-8253 Jordan-Dahlgren E, Maldonado MA, Rodriguez-Martinez RE (2005) Diseases and partial mortality in Montastraea annularis species complex in reefs with differing environmental conditions (NW Caribbean and Gulf of Mexico). Diseases of Aquatic Organisms 63:3-12

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