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Application note: Intermediate purification and polishing with Q PuraBead® Edge The choice of chromatographic resin is influenced by many factors particularly the stage of the workflow where the resin is to be used. Many downstream process developers are aware of the importance of selecting the right surface chemistry to purify the biological molecule of interest. However, it's common practice to focus primarily on surface chemistry (ligand) choices to deliver the desired level of purity without fully considering the crucial influence of the beaded support matrix. Many critical process parameters such as porosity, available surface area (capacity) and resistance to flow (back pressure) hinge on the properties of the selected chromatography base matrix. Therefore, when choosing the most suitable chromatography media for a particular application, it is essential to consider not only the ligand chemistry but also the stage of the workflow where the resin will be utilized, the nature of the feed being processed, and the processing speed required to achieve productivity goals.
PuraBead® P6: The Ideal Choice for Challenging Solutions. Resin beads of varying sizes play key roles in the chromatography unit operations of downstream processes. Larger beads such as those in the PuraBead® P6 series from Astrea Bioseparations are the resin of choice for handling complex feedstocks, particularly for initial product capture applications. The distinctive flow characteristics of the near-monodisperse 90 µm monodisperse PuraBead® P6 beads create sizable, uniform flow channels, facilitating rapid processing of solutions with minimal back pressure. Achieving high flow rates while maintaining low back pressures is important, especially during primary capture phases or when dealing with relatively viscous solutions such as plasma or plasma fractions. These scenarios often involve processing viscous feedstocks with a high concentration of proteins or other targets. Beads capable of forming a packed bed with extensive open flow channels are necessary for these tasks. In such applications, the wider elution peak shapes produced by larger beads are offset by the advantages of efficient sample purification, particularly for affinity chromatography where the resolution of sample components relies on binding selectivity not chromatographic performance.
Application note: Intermediate Purification and Polish with Q PuraBead® Edge
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