MiMode PuraBead® 96-well plates
Product code:
FG00619, FG00620, FG00621, FG00689, FG00690, FG00691, FG00692, FG00693
INTRODUCTION
The MiMode PuraBead® 96-well plate kit is a ready-to-use method development tool designed for high-throughput screening of mixed-mode chromatography conditions. Each well is pre-packed with 20 µL of Astrea Bioseparations Ltd and its affiliates’ (“Astrea Bioseparations”) mixed-mode chromatography resin.
Flexible operation
MiMode PuraBead® plates are compatible with both manual workflows (using multi-channel pipettes) and automated robotic systems. Their format supports high-throughput process development (HTPD) by enabling simultaneous screening of a wide range of chromatographic conditions.
Available formats
Plates are available in single-resin or multi-resin formats, offering flexibility for both targeted optimization and broader condition scouting. See the ‘ordering i nformation’ section for more details.
Accelerated development
By allowing parallel analysis of multiple variables, these plates help quickly identify optimal process conditions across a broad experimental space. Once target conditions are identified, scale-up and process optimization are easily performed using compatible pre-packed columns such as 1 mL & 5 mL HT pre-packed columns, Evolve® R 5 mL or 50 mL columns, or through bulk resin on ÄKTA™ systems.
Kit components
Each kit includes:
• One MiMode PuraBead® 96-well plate
• A wash plate
• A collection plate
These components provide everything needed to carry out parallel experiments efficiently.
Important product information
• For information on plate configuration of multi-resin mixed plates, please refer to the tables below.
• For resin-specific product information, specification, and resin-specific running conditions, please see the resin-appropriate Technical User Guide (TUG). This TUG will provide general running conditions recommended for a broad range screen.
MiMode PuraBead® Mixed- H 96-well plate configuration
MiMode PuraBead® HX1
MiMode PuraBead® HL4
MiMode PuraBead® HL3
MiMode PuraBead® HX2
MiMode PuraBead® Mixed- C 96-well plate configuration
MiMode PuraBead® CBX1
MiMode® CBX2
MiMode PuraBead® CX1
MiMode PuraBead® CX2
• If the purification is performed using centrifugation, the spin plate must be balanced throughout the procedure with a duplicate plate or a balance plate filled with an appropriate volume of water
• Empirically determine the optimal buffer (pH and salt concentration) for purifying and eluting the protein of interest based on the pI and hydrophobicity of the protein
When using MiMode PuraBead® 96-well plates with an automated system, follow instructions provided by the equipment manufacturer with regards to plate set-up and method programming.
Additional materials required
• Variable-speed centrifuge with rotor and carriers capable of handling stacked plates (4.4 cm height) at 500 × g, a vacuum manifold or positive pressure head system. Suggested flow rates are 4–8 inches Hg (2–4 psi), which is equivalent to 1–2 drops/second
• Multi-channel pipettor and tips
