Lentiviral vector purification spin columns
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2 | Nereus LentiHERO®
OUR TECHNOLOGY Shaping the future of cell and gene therapy manufacturing with AstreAdept®, a novel purification material. AstreAdept® brings fast flow rates and increased binding capacities to biological product purification. Higher capacity and faster runs compress bioprocessing, positively impacting on process economics. The compact nature of nanofibers results in smaller devices, reducing physical footprint, water usage, and waste removal, leading to greener manufacturing.
AstreAdept® enables extremely high flow rates without compromising on dynamic binding capacity High dynamic binding capacity BSA binding of 120 mg/mL
Bioprocessing using AstreAdept® AstreAdept® has highly accessible nanofibers which allows for almost immediate access to high binding surface area at high flow rates.
BSA breakthrough at 60mv/min 1s residence time
These unique features address the challenges of purifying large and fragile modalities which are often produced at low titers with high levels of contaminants. This technology has been incorporated into a spin column format, the Nereus LentiHERO® to bring these advantages to viral vector purification at lab-scale.
AstreAdept® nanofiber, functionalized with weak anion exchange, was tested using a chromatography system to characterize dynamic binding capacity and flow rate characteristics.
Nereus LentiHERO® | 3
Lentiviral Vector Purification Novel tools to reduce bottlenecks in viral vector processing Utilizing AstreAdept® technology, Nereus LentiHERO® has been shown to significantly reduce the bottlenecks of viral vector sample preparation by compressing the processing of multiple lab-scale lentiviral feedstocks. Combining the benefits of high lentiviral particle recovery, significant reduction of contaminants, and the ability to scale out, Nereus LentiHERO® is a novel alternative to affinity resins, IEX membrane adsorbers, and molecular weight cut off ultrafiltration filters.
Lentiviral vector purification using Nereus LentiHERO®
Key Features
• Easy-to-use spin column format with a simple 5 step workflow, used with a benchtop centrifuge.
• High binding capacity of 1.9E+10 lentiviral particles per unit
• No need for ultracentrifugation, large chromatography systems, or tedious filtering by hand.
• 95% reduction in host cell protein contamination
• Sample throughput is easily increased by maximizing benchtop centrifuge capacity. • Feedstock volumes are reduced in the order of 10fold depending on loading volume. • Purer samples with reduced volumes shortens duration of subsequent concentration steps, increasing throughput of feedstock processing.
1 unit for small or larger volumes
Easy-to-use spin column format
• > 60% recovery of lentiviral particles
• Lab-scale purification of lentiviral particles in 5 easy steps • Suitable for both serum and serum-free feedstocks
+44 (0) 1223 433 800 | astreabioseparations.com sales@astrea-bio.com | techsupport@astrea-bio.com | quality@astrea-bio.com Global bases in North America, Canada and Cambridge UK HQ: Horizon Park, Barton Road, Comberton, Cambridge, CB23 7AJ, UK
Nereus LentiHERO® - V2 This product is covered by or for use under one or more patents: www.astreabioseparations.com/patents All trademarks, trade names, trade dress, product names and logos appearing in this brochure are the property of Astrea UK Services Ltd. Copyright © 2022 Astrea Bioseparations Ltd. All rights reserved.