Newsletter: Volume 1- May 2023 CHANGES TO THE CCG
RECENT NEWS: The CCG has been awarded funds from the Roy J. Carver Charitable Trust to acquire new equipment to support the Department’s research efforts. We will be adding the following new equipment to the CCG in the next few months: Diagenode Bioruptor Pico II (Now Available!!!) BD FACS Melody Cell Sorter Bio Tek Synergy LX Multimode Plate Reader
We are excited to be upgrading some of our research equipment and adding some new equipment to extend the research mission of the Department of Biology. A few changes will be coming to the CCG over the next several months including billing and usage adjustments as well as updating our website. Also, the launch of this new newsletter format to inform you of the updates to the CCG and to highlight different equipment available for use. Email CCG (ccg@uiowa.edu) to be added to listserv for future newsletters.
Usage adjustments: Qubit is changing to a tiered billing based on usage per month (same cost as nanodrop) starting in May. You are required to log the number of samples you measure including any standards run on the sign-up sheet. You do not need to schedule time for the machine online unless you are doing a lot of samples at once or require use of it at a set time. Tissue Lyser LT is also changing to a tiered billing based on usage per month (same cost as Bioruptor) starting in May. You are required to log the number of RUNS you do, NOT samples. The machine holds 12 samples, so if you fill all slots and run it 3 cycles: you would log 3 runs.
Updating equipment: QuantStudio (QS3) machine received a computer hardware update to make the launching of the software faster. If you notice any problems, please email me (Christine) at ccg@uiowa.edu Nanodrop One machine also received a computer hardware update. As with the QS3, let me know if any problems occur. (ccg@uiowa.edu) Plate Reader: The old Spectramax M2 plate reader will be replaced with a NEW Bio Tek Synergy LX Multimode Plate Reader. It runs on the same software as the Cytation5 and will be highlighted in the next newsletter. We expect this instrument to be available for use starting in early June.
New Equipment Highlighted: Diagenode Bioruptor Pico II is a sonicator with a built-in cooling system to shear samples. This instrument can be used for: 1) DNA shearing for NGS, 2) Chromatin shearing, 3) RNA shearing, 4) Protein extraction from tissues & cells, 5) FFPE DNA extraction, 6) Protein aggregation studies, and 7) shearing of input DNA for NGS. We have purchased all the tube adaptors for the instrument to do a range of sample volumes from 20ul – 2ml (0.2ul, 0.65ul, 1.5ml, and 15ml Bioruptor Pico II