Newsletter: Volume 1- June 2023 CCG UPDATES
RECENT NEWS: The CCG has been awarded funds from the Roy J. Carver Charitable Trust to acquire new equipment to support the Department’s research efforts. We will be adding the following new equipment to the CCG in the next few months: Diagenode Bioruptor Pico II (Now Available!!!) BD FACS Melody Cell Sorter Bio Tek Synergy LX Multimode Plate Reader (Now Available!!!)
This is our second newsletter highlighting the newest equipment addition to the CCG: BioTek Plate Reader. If you have any questions and would like to be added to our listserv for future newsletters, email the CCG (ccg@uiowa.edu).
Updating equipment: Plate Reader: The NEW Bio Tek Synergy LX Multimode Plate Reader has come in and is set up in the CCG! You are welcome to start using it! Yippie! This instrument is replacing the old Spectramax M2 plate reader. (See Highlight below for more details.) NanoDrop One: A recent computer hardware update is finally complete. Now, you can log into the computer with your HawkID and run the NanoDrop via the desktop software. You can also export your data to your email. The system is much faster and the NanoDrop software has been updated as well. Please continue to sign the use/log sheet when using this machine regardless if you log into the computer. If you are experiencing any problems, email the CCG (ccg@uiowa.edu).
New Equipment Highlighted: Bio Tek Synergy LX Multimode Plate Reader is a microplate reader that does continuous UV-Vis absorbance measurements with wavelength range from 200-999nm in 1nm increments, allowing it to be used for numerous colorimetric and UV applications including nucleic acid and protein quantification, turbidity measurements, BCA and Bradford assays, cell viability assays, ELISA, and more. In addition to the absorbance measurements, this machine has the capability to do fluorescence and luminescence measurements extending its use to fluorescence ELISA assays, gene expression analysis such as determining transfection efficiency of GFPreporter genes or luciferase-based assays, cell viability assays with luminescence ATP, and other fluorescence-based assays.
The Synergy LX reader has 4 filter cubes with dichroic mirrors that can be easily swapped in and out of the system: 1) luminescence cube, 2) blue fluorescence cube with 360/40nm excitation filter, 460/40nm emission filter, and 400nm cut off dichroic mirror, 3) green fluorescence cube with Bio Tek Synergy LX plate reader 485/20nm excitation filter, a 528/20nm emission filter, and 510nm cut off dichroic mirror, and 4) red fluorescence cube with 530/25 excitation filter, 590/35nm emission filter, and 570nm cut off dichroic mirror. The Synergy LX reader can use 6- to 384-well plates and Take3 plates for microvolume analysis (16- 2ul samples). Lastly, the machine runs on the same software (Gen5) as the Cytation5.